Enrichment of lung cancer stem cells and expression of related markers

doi:10.3969/j.issn.2095-4344.2015.14.007

Abstract

Abstract:

BACKGROUND: Lung cancer stem cells are tightly related to the treatment and prognosis of lung cancer. We can provide more references for clinical diagnosis and treatment of lung cancer through the study on the tumorigenicity and surface markers of lung cancer stem cells.

OBJECTIVE: To explore the enrichment methods for lung cancer stem cells and cellular tumorigenicity.

METHODS: Lung cancer stem cells were induced in serum-free culture medium containing epidermal growth factor, insulin-like growth factor 1, and basic fibroblast growth factor. Then, the expressions of related surface markers were detected using immunofluorescence method. After that, mice were implanted subcutaneous with lung cancer stem cell spheres to understand the tumorigenicity of lung cancer stem cells.

RESULTS AND CONCLUSION: Lung cancer stem cells under serum-free induction and culture were changed to sphere-forming cells, and the immunofluorescence detection showed that over 80% of sphere-forming cells were positive for CCSP, SP-C and OCT4. After transplantation of sphere-forming cells, the mice showed a high tumorigenicity. These findings indicate that sphere-forming cells are formed after serum-free suspension culture of lung cancer stem cells, which have a higher tumorigenicity.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Lung Neoplasms, Neoplastic Stem Cells, Culture Media, Serum-Free, Insulin-Like Growth Factor I, Epidermal Growth Factor, Fibroblast Growth Factor 2

CLC Number:

R394.2

Chen Ping, Wang Jian.

Enrichment of lung cancer stem cells and expression of related markers

[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2167-2171.

Figures/Tables 1

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