Effect of Nd:YAG laser on the expression of basic fibroblast growth factor in inflammatory dental pulp

doi:10.3969/j.issn.2095-4344.2014.46.005

Abstract

Abstract:

BACKGROUND: The laser applied in the clinical oral medicine is mainly for early diagnosis of caries, removal of carious tissue, pulpotomy for pulp bleeding, dentin hypersensitivity treatment, disinfection of infected root canals, and periodontitis treatment.

OBJECTIVE: To explore the changes of basic fibroblast growth factor in inflammatory pulp after laser treatment.

METHODS: Five healthy adult beagle dogs were enrolled, and six teeth were randomly selected from each dog. Then, these teeth were randomly divided into three groups, 10 teeth in a group, and they were treated with laser irradiation, saline and gentamicin irrigation respectively. At days 1, 2, 3 after operation, exudates from the pulp were collected for detection of basic fibroblast growth factor expression. Meanwhile, the dental pulp was detected by hematoxylin-eosin staining and immunohistochemistry method.

RESULTS AND CONCLUSION: In the all three groups, the expression of basic fibroblast growth factor at day 3 was significantly higher than that at days 1 and 2, and there were no differences between days 1 and 2. Moreover, these three groups also showed no significant differences. Under hematoxylin-eosin and immunohistochemical staining, blood vessels in the pulp cavity were in good condition and the pulp arranged tightly. These findings suggest that the laser is safe, convenient and effective for oral clinical application, and has no injury to the inflammatory pulp.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: pulpitis, lasers, fibroblast growth factor 2

CLC Number:

R318

Mao Xiao-quan, Yu Dou, Cheng Ya-nan, Wang Yue-yan, Liu De-da. Effect of Nd:YAG laser on the expression of basic fibroblast growth factor in inflammatory dental pulp [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(46): 7401-7405.

Figures/Tables 2

References

[1] 林仰东,赵博,殷恺.碱性成纤维细胞生长因子对体外培养人牙周膜细胞的作用[J].中国组织工程研究,2014,18(20): 3184-3189.
[2] Pandiar D, Shameena P.Immunohistochemical expression of CD34 and basic fibroblast growth factor (bFGF) in oral submucous fibrosis. J Oral Maxillofac Pathol. 2014;18(2): 155-161.
[3] Yoo MG, Kim IH.Keloids and hypertrophic scars: characteristic vascular structures visualized by using dermoscopy. Ann Dermatol. 2014;26(5):603-609.
[4] 张笋,陈涛,葛立宏.应用Er:YAG 激光治疗儿童龋齿的效果评价[J].北京大学学报:医学版,2013,45 (1):87-91.
[5] Porcaro G, Amosso E, Scarpella R, et al.Doxycycline fluorescence-guided Er:YAG laser ablation combined with Nd:YAG/diode laser biostimulation for treating bisphosphonate-related osteonecrosis of the jaw. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014;10. S2212-4403 (14)00465-9.
[6] Firoozmand L,Faria R,Araujo MA,et al.Temperature rise in cavities prepared by high and low torque handpieces and Er: YAG laser.Br Dent J.2008;205(1): 28 -29.
[7] 郭怡丹,张笋.Er: YAG 激光治疗对牙髓的作用[J].中国激光医学杂志,2013,22(3):165-171.
[8] Colucci V,Amaral FL,Pecora JD,et al.Water flow on erbium: yttrium-aluminun-garnet laser irradiation: effect on dental tissues.Lasers.Med Sci.2009;24(5): 811-818.
[9] TakimotoK, KawashimaN, Suzuki N, et al.Down-regulation of Inflammatory Mediator Synthesis and Infiltration of Inflammatory Cells by MMP-3 in Experimentally Induced Rat Pulpitis. JEndod. 2014;40(9):1404-1409.
[10] 姚敏,方勇,王莹.激光医学与组织修复[J].上海交通大学学报:医学版,2010,13(12):1451-1454.
[11] 崔卫新,李云川,邵鸣凯,等.鼻内镜下射频及Nd:YAG激光治疗鼻出血临床效果观察[J].北京医学,2010,32 (2):103-106
[12] Schwarz F, Hegewald A, John G, et al.Four-year follow-up of combined surgical therapy of advanced peri-implantitis evaluating two methods of surface decontamination.J Clin Periodontol. 2013;40(10):962-967.
[13] 冯向辉,路瑞芳,和璐.应用Er: YAG激光治疗慢性牙周炎的短期临床疗效观察[J].北京大学学报:医学版,2011;43(6):886-890.
[14] Matsumoto A, Tamura A, Fukami K, et al. Single-pulse underwater laser-induced breakdown spectroscopy with nongated detection scheme.Anal Chem. 2013;85(8): 3807-3811.
[15] 林实,吴为良,詹振林,等.Er:YAG激光辐射后不同牙本质粘结界面的微观形态观察[J].中国激光,2011;38(3):121-126.
[16] 李鑫,陈新梅,郭蓝,等.Er:YAG激光联合3M ESPE Adper Easy－one粘结剂堵塞牙本质小管的体外研究[J].牙体牙髓牙周病学杂志,2012,22(2):92-95.
[17] 郭晓钰,吴友农,陈慧芬,等.Nd:YAg激光照射治疗牙本质敏感症的能量密度范围的离体牙研究[J].口腔医学研究,2014,30(5): 430-437.
[18] Lopes AO, Aranha AC.Comparative evaluation of the effects of Nd:YAG laser and a desensitizer agent on the treatment of dentin hypersensitivity: a clinical study.Photomed Laser Surg. 2013;31(3):132-138.
[19] 王华,赵彬,姚蔚.氧化锆陶瓷表面喷砂及牙本质表面Er:YAG激光处理对树脂黏结强度影响研究[J].中国实用口腔科杂志, 2013, 6(1):24-27.
[20] 李阳,贾兴亚,杜健男.表面处理方法对激光预备牙本质黏结强度的影响[J].中国实用口腔科杂志,2014;7(5):283-286.
[21] 王思箭.碱性成纤维细胞生长因子的生物活性及应用[J].中国医院药学杂志,2013;33(13):1081-1083.
[22] 张进,刘艳丽,孟秋菊,等.碱性成纤维细胞生长因子对人牙髓细胞生物学活性的影响[J].牙体牙髓牙周病学杂志,2013, 23(7) : 450-453.
[23] 关雪莲,侯丽淳,王辰,等.大鼠脑出血周边组织谷氨酸、脑水含量和细胞凋亡的变化及碱性成纤维生长因子的影响[J].中风与神经疾病杂志,2013,30(4):325-328.
[24] 赵姝哲,周婷婷,黄继红,等.低强度激光照射对实验性牙齿移动牙周组织中P物质表达的影响[J].中国激光医学杂志,2010, 19(3): 152-155.
[25] 刘琨,张惠民,申丽丽,等.Nd: YAG激光对3种树脂水门汀与牙本质粘接强度的影响[J].华西口腔医学杂志.2012,30(5):509-513
[26] 关为群,王学莹,杨宾.Nd:YAG激光治疗对慢性牙周炎患者牙周袋深度和附着丧失的改善效果研究[J].中国实用口腔科杂志, 2014,3(4):231-235.
[27] 关为群,杨宾,王清妹.Nd:YAG激光照射对人牙周膜成纤维细胞影响的体外研究[J].当代医学,2010,16(19):7-9.
[28] Ishikawa K, Maruyama T, Kusaka M, et al. The state of antimicrobial prophylaxis for holmium laser enucleation of the prostate: HoLEP and the results of a questionnaire survey. Hinyokika Kiyo.2011;57(10):539-543.
[29] 向学熔,晏莺,杨梅.Nd:YAG激光非手术疗法在老年牙周炎患者中的应用前景[J].中国实用口腔科杂志,2014,7(2):85-88.
[30] Ramalho KM, de Freitas PM, Correa-Aranha AC, et al.Lasers in esthetic dentistry: soft tissue photobiomodulation, hard tissue decontamination, and ceramics conditioning. Case Rep Dent. 2014;2014:927429.
[31] Kert J,Rose L.Clinical laser therapy low level laser therapy laser therapy[M].Denmark: Scandinavian Medical Laser Technology.1989: 5-6.
[32] Acar O, Tuncer D, Yuzugullu B, et al. The effect of dentin desensitizers and Nd:YAG laser pre-treatment on microtensile bond strength of self-adhesive resin cement to dentin. J Adv Prosthodont. 2014;6(2):88-95.
[33] 陆铃,李菊,刘鲁川.脉冲Nd:YAG激光对犬牙活髓切断术后组织修复的影响[J].四川医学,2010,31(12):1745-1747.