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    12 November 2014, Volume 18 Issue 46 Previous Issue    Next Issue
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    Ultrasonography for cartilage synovial disease of the knee in the elderly
    Luo Fei, Liang Min, Hou Zhi-mei, Zhang Ai-hong, Mayinuer Maimaiti, Liu Chun-hong
    2014, 18 (46):  7381-7385.  doi: 10.3969/j.issn.2095-4344.2014.46.001
    Abstract ( 364 )   PDF (677KB) ( 829 )   Save

    背景:超声作为无创性检查手段应用于骨骼肌系统检查已得到广泛的认识与认可。

    目的:探讨超声检查在老年人膝关节退行性变中的临床应用价值。
    方法:选取60岁及以上连续就诊的膝关节疾病患者102例,204个膝关节,根据膝关节退行性变临床诊断标准,分为2组:膝骨关节炎组167个膝关节,非膝骨关节炎组37个膝关节。行膝关节超声探查,观察膝关节髌上囊、内侧滑囊、外侧滑囊积液深度、滑膜、血管翳、软骨情况。
    结果与结论:204个膝关节中,发生膝关节退行性变的有167膝,原发性膝关节炎91膝,非原发性膝关节炎76膝,退变率82%,膝骨关节炎组膝关节腔髌上囊、内测滑囊、外侧滑囊积液量显著多于非膝骨关节炎组,滑膜增生59膝,伴有血管翳形成23膝,软骨表面毛糙167膝,软骨变薄或丧失117膝。非膝骨关节炎组滑膜增生5膝,软骨表面毛糙27膝,软骨变薄或丧失4膝。结果提示超声作为一种影像学检查手段对评价老年人膝关节退行性变具有实际的应用意义。


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Microendoscopic discectomy associated with annulus repair: comparison of early functional recovery of the lumbar spine  
    Li Chuan-jiang, Li Qing-chu, Wang Xiao-yong, Liu Ze-zheng, Yang Yang
    2014, 18 (46):  7386-7390.  doi: 10.3969/j.issn.2095-4344.2014.46.002
    Abstract ( 605 )   PDF (695KB) ( 1028 )   Save

    BACKGROUND: Studies have shown that limited lumbar discectomy can harvest better clinical efficacy, but also face a higher risk of recurrence. In clinic, how to guarantee access to good effect, and meanwhile to reduce the probability of recurrent disc herniation? The annulus repair technology may be an effective way, but it is rarely reported.

    OBJECTIVE: To investigate the early clinical effects of endoscopic lumbar discectomy associated with annulus repair in the treatment of lumbar disc herniation.
    METHODS: Totally 224 patients with lumbar disc herniation who accepted discectomy surgery were selected from the Department of Spinal Surgery, the Third Affiliated Hospital of Southern Medical University from January 2011 to January 2013, including 56 cases of microendoscopic discectomy associated with annulus repair (repair group) and 168 cases of microendoscopic discectomy (control group). Oswestry disability index and visual analog scale scores for lumbago and lower limb pain were recorded before and at 10 days, 3 months, 6 months, 12 months and 18 months after operation. Simultaneously, operative time, blood loss, surgical complications, and postoperative recurrence of lumbar disc herniation were recorded.
    RESULTS AND CONCLUSION: In the repair group, only 51 patients completed the follow-up, while all the patients in the control group completed the follow-up. There was no difference between the repair and control groups before and after surgery in the Oswestry disability index and visual analog scale scores for lumbago and lower limb pain (P > 0.05), but at 10 days after surgery, the Oswestry disability index and visual analog scale scores for lumbago and lower limb pain were significantly decreased in the repair group (P < 0.05), and this trend continued until the 18th month after surgery. There were no dural tears, disc space infection, hematoma formation in the spinal canal and other serious complications. The recurrence rate was 9.5% in the control group and 3.9% in the repair group. 31.2% of relapsed patients in the control group received the second operation, while on patient in the repair group received reoperation. These findings indicate that microendoscopic discectomy associated with annulus repair can obtain remarkable early clinical results, and effectively reduce the recurrent rate and risk of secondary lumbar disc surgery, which is safe and reliable.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effect of Uighur medicine abnormal savda munzip on hypertrophic scar and transforming growth factor beta/Smad signal transduction pathway  
    Li Nan, Kong Meng-long, Ma Tao, Li Jia-fu, Ma Shao-lin
    2014, 18 (46):  7391-7395.  doi: 10.3969/j.issn.2095-4344.2014.46.003
    Abstract ( 276 )   PDF (1968KB) ( 370 )   Save

    BACKGROUND: Previous observations have suggested that abnormal savda munziq has a possible anti-scaring effect. However, the precise pathological mechanism still remains unknown. Here, it is considered whether abnormal savda munziq can alleviate the formation of hypertrophic scar by affecting the expression of transform growth factor β (TGF-β)/Smad signal transduction pathway.

    OBJECTIVE: To evaluate the in vitro effect of abnormal savda munziq on the proliferation and TGF-β/Smad signal transduction pathway in hypertrophic scar fibroblasts.
    METHODS: Hypertrophic scar fibroblasts were divided into six groups to receive different treatments as group A (blank control group), groups B-E (0.2, 0.4, 0.6, 0.8, 1.0 g/L abnormal savda munziq). Each group contained six specimens. After intervention, the MTT test, flow cytometry and immunocytochemistry methods were used to detect the alteration of cell proliferation and TGF-β/Smad signal transduction pathway.
    RESULTS AND CONCLUSION: The inhibition effect of abnormal savda munziq (0.2-0.8 g/L) on the fibroblasts enhanced in a concentration-dependent manner than the blank control group (P < 0.05). The immunocytochemistry results showed that the expression of Smad7 increased while expression of TGF-β1 decreased in hypertrophic scar fibroblasts with abnormal savda munziq. These findings suggest that the reduction on the proliferation caused by abnormal savda munziq could be attributed to its effects on TGF-β/Smad signal transduction pathway. Abnormal savda munziq could be used as an effective drug for treatment of hypertrophic scar.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Culture and identification of chondrocytes isolated from the vertebral endplate of patients with type I neurofibromatosis associated with atrophic changes in vitro  
    Liu Xue-guang, Qiu Yong, Sun Zhen-zhong, Qian Bang-ping, Wang Shou-feng
    2014, 18 (46):  7396-7400.  doi: 10.3969/j.issn.2095-4344.2014.46.004
    Abstract ( 341 )   PDF (2174KB) ( 413 )   Save

    BACKGROUND: Previous studies have mainly focused on costal cartilage, articular cartilage, nasal septal cartilage, and auricular cartilage, but in vitro culture of human vertebral endplate cartilage is still rarely reported.

    OBJECTIVE: To discuss the feasibility of culture of vertebral endplate chondrocytes from type I neurofibromatosis associated with scoliosis patients in vitro and to study the biological characters of the chondrocytes.
    METHODS: Through two-step enzymatic digestion and tissue culture, the chondrocytes from the vertebral endplate of seven type I neurofibromatosis patients isolated and cultured in monolayer and passaged to observe the changes of cell morphology under inverted phase contrast microscope. Collagen type II expression was detected by immunocytochemistry to identify whether the cells had chondrocyte characters. The growth kinetics was detected by using MTT colorimetric assay to draw the growth curve of passage 2 chondrocytes.
    RESULTS AND CONCLUSION: A few chondrocytes crawled from the cartilage after 2 weeks culture and cells were passaged at 3 weeks. Along with passage going on, the phenotype of chondrocytes was changed from polygonal, round, triangle, and irregular shapes to fusiform. The collagen type II expression in passage 2 cells was positive by immunohistochemical staining. MTT test showed the growth curve of the passage 2 chondrocytes presented a transverse “S”. Cells were found logarithmic growth at days 4-7, reached platform stage at days 8-13, and decreased at day 14. It is an effective and simple procedure by two-step enzymatic digestion and tissue explant method to culture vertebral endplate chondrocytes with high purity and good viability from type I neurofibromatosis patients associated with scoliosis in vitro. Passage 2 chondrocytes from the vertebral endplate exhibit the best viability at days 4-7, which can be used as targets for research of pathogenesis of type I neurofibromatosis with atrophic scoliosis.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effect of Nd:YAG laser on the expression of basic fibroblast growth factor in inflammatory dental pulp  
    Mao Xiao-quan, Yu Dou, Cheng Ya-nan, Wang Yue-yan, Liu De-da
    2014, 18 (46):  7401-7405.  doi: 10.3969/j.issn.2095-4344.2014.46.005
    Abstract ( 381 )   PDF (1995KB) ( 409 )   Save

    BACKGROUND: The laser applied in the clinical oral medicine is mainly for early diagnosis of caries, removal of carious tissue, pulpotomy for pulp bleeding, dentin hypersensitivity treatment, disinfection of infected root canals, and periodontitis treatment.

    OBJECTIVE: To explore the changes of basic fibroblast growth factor in inflammatory pulp after laser treatment.
    METHODS: Five healthy adult beagle dogs were enrolled, and six teeth were randomly selected from each dog. Then, these teeth were randomly divided into three groups, 10 teeth in a group, and they were treated with laser irradiation, saline and gentamicin irrigation respectively. At days 1, 2, 3 after operation, exudates from the pulp were collected for detection of basic fibroblast growth factor expression. Meanwhile, the dental pulp was detected by hematoxylin-eosin staining and immunohistochemistry method. 
    RESULTS AND CONCLUSION: In the all three groups, the expression of basic fibroblast growth factor at day 3 was significantly higher than that at days 1 and 2, and there were no differences between days 1 and 2. Moreover, these three groups also showed no significant differences. Under hematoxylin-eosin and immunohistochemical staining, blood vessels in the pulp cavity were in good condition and the pulp arranged tightly. These findings suggest that the laser is safe, convenient and effective for oral clinical application, and has no injury to the inflammatory pulp.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    A comparative analysis of MRI and arthroscopy in meniscus injury of the knee joint  
    Wang Cheng-wei, Liu Li-bing, Jia Wei-dong, Zhao Bo, Paerhati, Wang Xue, Li Lu-bing, Zheng Hui
    2014, 18 (46):  7406-7411.  doi: 10.3969/j.issn.2095-4344.2014.46.006
    Abstract ( 651 )   PDF (736KB) ( 591 )   Save

    BACKGROUND: MRI characterized as non-invasion, high resolution, high sensitivity and specificity to injury has become another important means for diagnosis of knee joint disease following the arthroscopy.

    OBJECTIVE: To explore the effective evaluation on the knee meniscus injury diagnosis, by comparing the results of MRI and arthroscopy of the knee meniscus.
    METHODS: Clinical data of 206 patients with meniscus injury were retrospectively analyzed. Every case was subjected to MRI and the arthroscopy. The findings of arthroscopy were considered as the golden standard. Then, the sensitivity, specificity and coincidence rate of MRI in the diagnosis of meniscus injury were calculated. The chi-square analysis was used to evaluate the MRI and arthroscopy in the diagnosis of meniscus injury. The Kappa values were used to test the consistency of MRI with arthroscopy in the diagnosis of meniscus injury.
    RESULTS AND CONCLUSION: For levels 0 and I meniscus injury, the coincidence rate of MRI was 97%; for level II meniscus injury, the coincidence rate of MRI was 91.1%, and there was no significant difference (P > 0.05). For level III meniscus injury, the sensitivity, specificity, concordance rate and Youden index of MRI were 92.9%, 94.4%, 93.7% and 87.3%, respectively. By the consistency checking, the value of Kappa was 0.874 and χ2 was 38.182, so there was a high consistency checking between the data of MRI and arthroscopy (P=0.000). MRI is a noninvasive diagnostic method for meniscus injury. For patients with levels I and II meniscus injury on MRI, arthroscopy should be cautiously adopted; however, for patients with level III meniscus injury on MRI, arthroscopy should be done as early as possible. Arthroscopic observation is more intuitive that enables to make accurate judgments of meniscus injury to reduce the rate of misdiagnosis.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Percutaneous transforaminal endoscopic discectomy combined with radiofrequency annuloplasty for treatment of lumbar disc herniation   
    Zhang De-hong, Fang Peng-fei, Yan Yong-hai
    2014, 18 (46):  7412-7416.  doi: 10.3969/j.issn.2095-4344.2014.46.007
    Abstract ( 795 )   PDF (816KB) ( 734 )   Save

    BACKGROUND: Percutaneous transforaminal endoscopic discectomy combined with radiofrequency annuloplasty is a minimally invasive therapy for chronic discogenic low back pain, especially suitable for lumbar disc herniation.

    OBJECTIVE: To evaluate the safety and short-term efficacy of percutaneous transforaminal endoscopic discectomy combined with radiofrequency annuloplasty for treatment of lumbar disc herniation.
    METHODS: Thirty-two patients with lumbar disc herniation were subjected to percutaneous transforaminal endoscopic discectomy combined with radiofrequency annuloplasty. Visual analogue scale and modified Macnab criteria were used to evaluate the relief of low back pain, and the Oswestry Disability Index was used for daily life evaluation of patients. All the patients were followed for 1-6 months, with an average of 3.5 months.
    RESULTS AND CONCLUSION: The operation was successfully completed in all patients, and no complications such as damage to nerve and vascular systems occurred. Low back pain and living ability of the patients were improved obviously. Visual analog scale score and the Oswestry Disability Index were improved significantly after operation (P < 0.01); according to Macnnab's criteria, the results showed that at 1 week after the operation, the effective rate reached 94%; at 3 months after the operation, the effective rate was up to 97%. These findings indicate that percutaneous transforaminal endoscopic discectomy combined with radiofrequency annuloplasty in the treatment of lumbar disc herniation is confirmed in the short-term efficacy, and characterized as fewer complications and stable functional recovery.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Thin-thickness versus blade-thickness micro-skin pulping covered with heterogeneous skin for repair of burn wounds  
    Zhang Ming-zhu, Wang Jian-hua, Li Hu, Dong Yun-feng, Qi Chang-chun, Guo Bao-wen, Wang Yong-ling, Liu Xiao-yan, Li Yun-feng, Zhang Xiao-hui, Liu Ying, Li Xing-hua, Wang Hong-feng, Yu Qiang
    2014, 18 (46):  7417-7421.  doi: 10.3969/j.issn.2095-4344.2014.46.008
    Abstract ( 377 )   PDF (723KB) ( 501 )   Save

    BACKGROUND: Autologous micro-skin graft covered with allogeneic skin has become a major means for repair of extremely severe burn wounds. Due to the limitation of the source of allogeneic skin, heterogeneous skin (pigskin) or artificial skin serves as a vector to cover the wound on the clinical use.

    OBJECTIVE: To observe the curative effect of thin-thickness micro-skin pulping covered with heterogeneous skin for repair of burn wounds in comparison with blade-thickness micro-skin pulping covered with heterogeneous skin.
    METHODS: A self-control study was performed in 36 patients with severe burns of bilateral limbs. There were 42 pairs of wounds, 32 of which were at joint sites. One or two pairs of III-degree burns treated with escharectomy were selected from each patient for self-control comparison. Covered with heterogeneous skin, thin-thickness micro-skin pulping and blade-thickness micro-skin pulping were used in the trial and control groups, respectively. The expansion ratio was controlled at 1:8-1:12 in all patients. Wound healing time, degree of scar hyperplasia within 6 months to 2 years after transplantation, and ratio of reconstruction surgery at joint sites were observed and compared. The removed scars by surgery were analyzed based on the total score of the Vancouver Scar Scale.
    RESULTS AND CONCLUSION: The average wound healing time was (44.7±1.24) days in the trial group and (49.6±1.41) days in the control group, and there was a significant difference between the two groups (P < 0.05). The trial group exhibited less scar hyperplasia than the control group at 6 months to 2 years after transplantation. Compared with the control group, the ratio of severe scar hyperplasia was significantly less in the trial group (P < 0.05), while the ratio of mild scar hyperplasia was obviously greater in the trial group (P < 0.01). However, there was no difference in the ratio of moderate scar hyperplasia between the two groups (P > 0.05). The reconstructive surgery ratio of the trial group was 38%, significantly lower than that of the control group (59.38%; P < 0.01). The total score on the Vancouver Scar Scale was less in the trial group than the control group (P < 0.05). These findings suggest that the thin-thickness micro-skin covered with heterogeneous skin is likely to be a better treatment to repair large full-thickness skin burn by increasing the thickness of micro-skin, and it can obtain better wound healing quality.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Digital replantation by Flow-through flap from the fibular side of great toe  
    Luo Deng-ke, Pan Zhen-yu, Cheng Ke-ke, Yu Ai-xi
    2014, 18 (46):  7422-7426.  doi: 10.3969/j.issn.2095-4344.2014.46.009
    Abstract ( 401 )   PDF (695KB) ( 431 )   Save

    BACKGROUND: The severity of tissue injury varies with the causes of the amputated finger. Simple soft tissue, vessels or nerves injury could be easily repaired by adjacent finger flap or abdominal flaps. However, these treatments are short of long repair time, reoperation and unsatisfactory appearance of the finger.

    OBJECTIVE: To investigate the efficiency of Flow-through flap from the fibular side of great toe to repair skin and soft tissue defects in digital replantation.  
    METHODS: From January 2011 to October 2013, Flow-through flap from the fibular side of great toe was applied to repair soft tissue injury in digital replantation for 11 cases (8 males and 3 females, age ranged from 23 to 42 years. Skin defects ranged from 2.0 cm ×1.5 cm to 4.0 cm×2.2 cm; vascular defect ranged from 1-3 cm, 1.5 cm averagely; and the flap size ranged from 2.2 cm×1.7 cm to 4.5 cm×2.5 cm. 
    RESULTS AND CONCLUSION: The follow-up time of all patients was 6-18 months. Digital replantation was successful in all the 11 cases. Ten cases were healed by first intention, and one case was gradually rescued after dressing change. Patients were satisfied with the flap and the peripheral sensation. The peripheral discrimination of patients was 4 to 10 mm. The fingers functioned well in flexion and extension. Of the 11 cases, 9 cases were valued excellent and 2 cases was rated as good, according to the upper extremity function evaluation standard of the Hand Surgey Branch of Chinese Medical Association. Flow-through flap from the fibular side of great toe is an ideal method to repair skin soft tissue and vascular defects in digital replantation.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Application of striping and local injections in patients with auricle keloid  
    Tang Jie, Li Xiao-jing, Tang Yue-ling, Ning Jin-long
    2014, 18 (46):  7427-7431.  doi: 10.3969/j.issn.2095-4344.2014.46.010
    Abstract ( 446 )   PDF (607KB) ( 603 )   Save

    BACKGROUND: Keloids are the result of abnormal post-traumatic skin restoration, which are characterized with overgrowth and progressive enlargement, invasion of adjacent normal skin tissue, extension beyond the scope of the original wound. Given the high recurrence, treatment with surgery alone is meaningless and, therefore, comprehensive treatment of surgery and other approaches is required.

    OBJECTIVE: To explore the clinical curative effect of local injection after timely removal of auricle keloid core.
    METHODS: Twenty patients with auricle keloid were enrolled. After keloid core excision, the time interval within the incision scar tissue injection of fluorouracil or triamcinolone acetonide add lidocaine or monotherapy was gradually lengthened, and if necessary the proper amount of compound betamethasone was added according to scar conditions. The injection dose was adjusted timely based on the regression of scar. The treatment effect was observed for 12 to 24 months.
    RESULTS AND CONCLUSION: Of 36 auricle keloids from 20 patients, the comprehensive treatment was effective in 1 keloid, and other 35 keloids were cured. The effective rate was 100%, the completely cured rate was 97.2% and the excellent rate was 2.8%. These findings indicate that minimally invasive excision of keloid core and the epithelial flap coverage combined with 5-fluorouracil and corticosteroid injection is a safe and effective method to treat auricle keloids.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Detection of the calcium and phosphorus in the hard tissue of permanent teeth: spectrophotometry and ethylene diamine tetraacetie acid titration
    Ma Hai-liang, Yu Xin-bo, Liu Meng-dong, Jia Jing, Fu Da-yong
    2014, 18 (46):  7432-7436.  doi: 10.3969/j.issn.2095-4344.2014.46.011
    Abstract ( 349 )   PDF (289KB) ( 551 )   Save

    BACKGROUND: Currently, acid etching and bonding technology have been widely used in clinical stomatology. Data have indicated that the main content of inorganic elements (calcium and phosphorus) has a certain difference between the dental enamel and dentin of the young and adult permanent teeth.

    OBJECTIVE: To measure the content of main inorganic elements, calcium and phosphorus, in the dental enamel and dentin from young and adult permanent teeth with spectrophotometry and ethylene diamine tetraacetie acid titration method.
    METHODS: Each 20 adult and young permanent teeth in vitro were selected. Plaster stone and water was mixed; when it was nearly dried, the teeth were vertically cast in the mixture and the tooth surface was exposed. The models were cut into the slices using syj-200 precision cutting machine, and then the slices were put into nitric acid and dissolved through heater to prepare standard solution. At last, the concentrations of calcium and phosphorus in the hard tissue of both young and adult permanent teeth were measured with spectrophotometric method and titration method. 
    RESULTS AND CONCLUSION: The content of calcium and phosphorus and calcium/phosphorus ratio in the hard tissue of young permanent teeth were less than those of adult permanent teeth (P < 0.05), reflecting that the organic matter content was more than that in adult permanent teeth, but their mineralized degree was inferior to that of adult permanent teeth (P < 0.05). These findings indicate that the young permanent teeth are more acid proof than the adult permanent teeth; therefore, the acid etching time can be properly prolonged for young permanent teeth in clinical treatment, in order to achieve better effects.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of Dragon’s blood extracts on fibroblast proliferation and procollagen type III  
    Li Dan, Hui Rui, Hu Yong-wu, Han Yan, Guo Shu-zhong
    2014, 18 (46):  7437-7441.  doi: 10.3969/j.issn.2095-4344.2014.46.012
    Abstract ( 510 )   PDF (742KB) ( 610 )   Save

    BACKGROUND: Dragon’s blood is the main ingredient of traditional medicine prescription for promoting granulation, which has been used in clinical treatment of a variety of refractory wounds and achieved the exact effects. But the Dragon’s blood effect on collagen secretion from normal fibroblasts has not been reported.

    OBJECTIVE: To investigate the effects of Dragon’s blood extract on the proliferation and secret function of fibroblasts in vitro.
    METHODS: Dragon’s blood was extracted by extracts chloroform, acetoacetic ester, and alcohol in turn. Normal human fibroblasts were respectively cultured in Dragon’s blood extracts of chloroform, acetoacetic ester, and alcohol, DMEM containing 1% dimethyl sulfoxide, and normal culture medium. Then, the fibroblasts were cultured in vitro in different media containing gradient dilutions of Dragon’s blood extracts (0.002, 0.02, 0.2, 2, 20 g/L), which was followed by cell proliferation determination assessed with MTT assay. Under the optimal concentration, the cell growth curves were drawn and the flow cytometry was used to determine the changes of cell cycle. The concentration of procollagen type III in the supernatant of the fibroblast culture systems was measured by radioimmunoassay.
    RESULTS AND CONCLUSION: 0.2 g/L-2 g/L dilution of Dragon’s blood extracted by acetoacetic ester enhanced the proliferation of fibroblasts in a dose-dependent manner. The 2 g/L was the optimal dilution of Dragon’s blood extracted by acetoacetic ester, and it increased the ratio of S cells in cell cycle than control group and decreased procollagen type III. These findings indicate that Dragon’s blood acetoacetic ester extract can improve the proliferation of cultured human fibroblasts in vitro, and decrease the secretion of procollagen type III of fibroblasts, and it can be beneficial to improve wound healing and inhibit hypertrophic scar.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Autologous and allogeneic tendons for anterior cruciate ligament reconstruction  
    Yaxiaer• Sulaiman, Zhang Ke-yuan
    2014, 18 (46):  7442-7446.  doi: 10.3969/j.issn.2095-4344.2014.46.013
    Abstract ( 432 )   PDF (656KB) ( 465 )   Save

    BACKGROUND: There is a controversy in the clinical anterior cruciate ligament reconstruction using autologous hamstring tendon or tendon allograft.

    OBJECTIVE: To compare the clinical results of anterior cruciate ligament reconstruction with tendon allograft and autograft.
    METHODS: Totally 123 patients with injured anterior cruciate ligament received anterior cruciate ligament reconstruction and they were divided into two groups: 63 cases in autograft group and 60 cases in allograft group. The postoperative effects were assessed by the Lysholm score and Lachman test.
    RESULTS AND CONCLUSION: All the patients were followed for more than 8 months. After anterior cruciate ligament reconstruction, the stability of the knee joint, Lysholm scores and Lachman test results were all improved significantly in the two groups; while there was no difference between the two groups at the last follow-up      (P > 0.05). The clinical outcomes of anterior cruciate ligament reconstruction with allograft and autograft are almost similar; therefore, surgeons should select ideal graft according to the patient’s condition and surgeon’s experience.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Angiotensin II-transient receptor potential channel C6 signaling pathway mediates podocyte injury   
    Yao Dan-dan, Ma Rui-xia, Zhai Li-hui, Li Zuo-lin, Li Zhen
    2014, 18 (46):  7447-7451.  doi: 10.3969/j.issn.2095-4344.2014.46.014
    Abstract ( 374 )   PDF (606KB) ( 378 )   Save

    BACKGROUND: Transient receptor potential channel C6 (TRPC6) is a new and important slit diaphragm-associated protein in podocytes involved in regulating glomerular filter function. Glomerular TRPC6 expression is closely associated with proteinuria in diabetic kidney disease.

    OBJECTIVE: To investigate the expression of canonical TRPC6 in mouse podocytes induced by high glucose, and to explore the possible mechanism of diabetic kidney disease.
    METHODS: Mouse podocyte cells were cultured and divided into normal glucose group (5.6 mmol/L D-glucose), normal control group (5.6 mmol/L D-glucose+25 mmol/L mannitol) and experimental groups which were in the environment of high glucose (30 mmol/L). The experimental groups included high glucose group, valsartan treatment groups (10-5 mol/L) and U73122 control group (10 μmol/L U73122). After 48 hours, the expressions of mRNA and proteins of TRPC6, nephrin and angiotensin II (AngII) were detected respectively by real-time quantitative PCR and western blot analysis.
    RESULTS AND CONCLUSION: Compared with the normal control group, the expressions of mRNA and proteins of TRPC6 and angiotensin II were markedly elevated in the high glucose group (P < 0.01), while the expressions of mRNA and proteins of nephrin were decreased (P < 0.01). The mRNA and proteins of TRPC6 and angiotensin II expressions were significantly down-regulated by valsartan (P < 0.05, P < 0.01), while the mRNA and protein expressions of nephrin were effectively up-regulated (P < 0.05). Compared with the high glucose group, the expressions of mRNA and proteins of TRPC6 and angiotensin II were ameliorated in the U73122 control group. The expressions of mRNA and proteins of TRPC6, nephrin and angiotensin II had no statistical significance between the normal control group and normal glucose group (P > 0.05). Angiotensin II-TRPC6 signaling pathway may mediate high glucose-induced podocyte injury, meanwhile it provides a new theoretical basis for the treatment of diabetic kidney disease, by which the angiotensin receptor blockers can protect podocytes in diabetic kidney disease.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Expression of fibroblast growth factor, transforming growth factor and connective tissue growth factor in lumbar ligamentum flavum hypertrophy
    Jiang Yu-quan, Liu Ji-chun, Ye Xiao-jian, Hu Yue, Qu Jin-tao
    2014, 18 (46):  7452-7457.  doi: 10.3969/j.issn.2095-4344.2014.46.015
    Abstract ( 301 )   PDF (628KB) ( 411 )   Save

     BACKGROUND: Ligamentum flavum hypertrophy is one of the most important factors of lumbar spinal stenosis, but the molecular mechanism is still not very clear.

    OBJECTIVE: To explore the role of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 in hypertrophy of the lumbar ligamentum flavum.
    METHODS: The ligamentum flavum samples were divided into three groups according to different diseases: control group (acquired from the patients with lumbar spinal canal tumor, n=6), lumbar disc herniation (LDH) group (acquired from the patients with LDH, n=6) and lumbar spinal stenosis (LSS) group (acquired from the patients with LSS, n=6). Then the mRNA expressions of basic fibroblast growth factor, connective tissue growth factor, transforming growth factor β1 and collagen I, III, V of the ligamentum flavum were detected using real-time quantitative RT-PCR method. The roles of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 were explored.
    RESULTS AND CONCLUSION: The expression of basic fibroblast growth factor mRNA in the LSS group was significantly higher than that in the LDH and control groups (both P < 0.05); the expression of connective tissue growth factor mRNA was not found statistically different among the three groups, although it was slightly higher in the LSS group (P > 0.05); the expression of transforming growth factor β1 mRNA was significantly higher in the LSS group than in the LDH and control groups (both P < 0.01). The collagen I mRNA expressed significantly higher in the LSS group than the LDH and control groups (both P < 0.05), but both the collagen III and V mRNA showed no significant difference among the three groups (P > 0.05). This study indicate that both basic fibroblast growth factor and transforming growth factor β1 play important roles in the formation process of the lumbar ligamentum flavum hypertrophy, and the main type of the collagen in the hypertrophied ligamentum flavum is collagen I.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Primary revision after failure of anterior cruciate ligament reconstruction  
    Kong Ying, Wang Guo-dong, Zhang Yuan-min, Zhao Xiao-wei, Zhang Yu-ge
    2014, 18 (46):  7458-7462.  doi: 10.3969/j.issn.2095-4344.2014.46.016
    Abstract ( 2157 )   PDF (674KB) ( 905 )   Save

    BACKGROUND: Failure of anterior cruciate ligament reconstruction can be caused by multiple factors, which requires a revision surgery. However, there are few studies about the revision of anterior cruciate ligament.

    OBJECTIVE: To investigate the primary cause, indication, methods and effectiveness of revision after the failure of anterior cruciate ligament reconstruction.
    METHODS: Thirty patients with instability undergoing anterior cruciate ligament reconstructions were subjected to revision under arthroscopy. After revision, a systemic analysis was performed based on KT-2000 examination, the International Knee Documentation Committee (IKDC) scale, Lysholm and Tegner scores.
    RESULTS AND CONCLUSION: Of the 30 cases, 9 cases were reconstructed with bone-patellar tendon-bone autograft, 14 cases with hamstring tendon autograft, 7 cases with hamstring tendon allograft. Twelve cases had a femoral tunnel in the front of the predicted one, 1 case had knee stiffness, 8 cases had a tibial tunnel in the front of the predicted one, and 8 cases had both the femoral and tibial tunnels in the front of the predicted ones. One case had a malposited interference screw which reconstructed with bone-patellar tendon-bone autograft; one case complicated by posteromedial corner injury was not reconstructed. The reconstructed anterior cruciate ligament was absolutely ruptured and absorbed in 12 cases, and 18 cases had obviously loosen but still partly linked reconstructed ligament. None had severe incompletion of spongy bone. All cases received primary revision. Ten cases reconstructed with ipsilateral hamstring tendon, 14 cases reconstructed with contralateral hamstring tendon, and 6 cases with LARS ligament. The mean side-to-side difference of KT-2000 examination at 90° and 30°, the mean IKDC, Tegner and Lysholm scores were significantly improved after revision. There were many reasons leading to failure of anterior cruciate ligament reconstruction, such as malposition of the bone tunnel, invalid fixation, adhesion, compound ligament injuries, and ankylosis. The functional results and objective stability could be obtained through revision following anterior cruciate ligament reconstruction.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Autogenous tendon transplantation for repair of grade III medial collateral ligament injury   
    Zhu Wei-hong, Chen You, Wang Wan-chun, Huang Guo-liang, Chou Ke, Chen Xian-li
    2014, 18 (46):  7463-7467.  doi: 10.3969/j.issn.2095-4344.2014.46.017
    Abstract ( 346 )   PDF (628KB) ( 606 )   Save

    BACKGROUND: Young patients with cruciate ligament injury and high activity levels are prone to grade III medial collateral ligament injury in knee joint. Conventional construction methods such as direct restoration of injured ligaments and muscle-tendon transposition may induce the loosening of medial collateral ligament complex.

    OBJECTIVE: To explore the clinical effect of autologous tendon transplantation for grade III medial collateral ligament injury of the knee joint.
    METHODS: Forty-seven patients with grade III medial collateral ligament rupture were treated by operative repair and reconstruction. After repairing the anatomical structure of medial collateral ligaments, we liberated organic semitendinous tendon, bone-patella tendon-bone, and hamstring tendon, to reconstruct superficial medial collateral ligament.
    RESULTS AND CONCLUSION: Thirty-seven patients were followed up for 48 months. Preoperative Lysholm score, IKDC score, Tegner score at the final follow-up, and the widened medial joint space on valgus stress X-Ray films were significantly improved compared with before reconstruction (P < 0.05). According to clinical efficacy scores, 12 cases achieved excellent reconstruction, 18 cases achieved good reconstruction, 4 cases achieved moderate reconstruction, and 3 cases achieved poor reconstruction. The excellent and good rate was 82%. Medial collateral ligament reconstruction based on repairing the anatomical structure is effective and satisfactory. Autologous bone-patella tendon-bone or semitendinous tendon is a good choice for medial collateral ligament reconstruction.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Isokinetic muscle strength test for the muscular force after repair of ruptured Achilles tendon
    Zhao Wei-xia, Wu Zong-de, Liu Bo, Liu Hui
    2014, 18 (46):  7468-7471.  doi: 10.3969/j.issn.2095-4344.2014.46.018
    Abstract ( 582 )   PDF (461KB) ( 765 )   Save

    BACKGROUND: Restoration of tendon integrity and tenacity, and maintenance of ankle joint flexibility, play a crucial role on the rupture of Achilles tendon.

    OBJECTIVE: To evaluate the muscular force using isokinetic muscle strength test after the ruptured Achilles tendon is repaired, and to assess the repairing effect.
    METHODS: Muscle power including peak torque, peak torque/body weight, total work, average peak torque, set total work of flexor and extensor of 17 patients with Achilles tendon rupture were assessed after reconstruction using Biodex system 4 at 60 (°)/s and 120(°)/s.
    RESULTS AND CONCLUSION: The peak torque, peak torque/body weight, total work, average peak torque, set total work of flexor muscle group at the injured side were significantly decreased compared with uninjured side at 60 (°)/s and 120 (°)/s (P < 0.05). Isokinetic muscle strength test can reflect the muscular force after the ruptured Achilles tendon is repaired, and evaluate the repairing effect.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Establishing a hemodialysis vascular access with autologous vein transplantation  
    Zhang Fan, Wang Tao, Wu Xiao-bo, Zhu Jun, Cheng Yue, Mo Li-wen
    2014, 18 (46):  7472-7476.  doi: 10.3969/j.issn.2095-4344.2014.46.019
    Abstract ( 470 )   PDF (596KB) ( 604 )   Save

    BACKGROUND: Currently, literatures about autologous vein transplantation are few, and the research on the effect of different parts of autologous vein transplantation are not found yet.

    OBJECTIVE: To summarize the experiences of establishing the fistula using autologous vein transplantation so as to investigate the method of improving the success rate of surgery.
    METHODS: We analyzed retrospectively the data of 40 cases of establishing the fistula using autologous vein transplantation, and then compared the successful rate of autologous vein transplantation fistula, blood flow and operating time, thereby analyzing the influence of diabetes mellitus on the successful rate of autologous vein transplantation fistula.
    RESULTS AND CONCLUSION: The successful rates of autologous vein transplantation fistula at different parts ranging from high to low were as follows: the cephalic vein, great saphenous vein, basilic vein and small saphenous vein. Blood flow of the upper limb for vein transplantation fistula was obviously higher than that of the lower limb (P < 0.05). The operating time of autologous vein transplantation fistula was longer in the upper limbs than in the lower limbs (P < 0.01). For patients with diabetes mellitus, the successful rate of autologous vein transplantation was markedly lower than those with no diabetes mellitus (P < 0.01). For the hemodialysis patients with poor upper limb superficial vein, autologous vein transplantation is a better way of establishing the vascular access. Vein transplantation of the upper limbs is better than that of the lower limbs in success rate and operating time. Autologous vein transplantation fistula is not suitable for the patients with diabetes mellitus.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    The function of Chinese herbal medicine in the reconstruction of periodontal tissue during orthodontic tooth movement
    Chen Ya-zhu, Guan Xiao-yan, Xiao Qian-wen, Wang Si-wei, Liu Jian-guo
    2014, 18 (46):  7477-7481.  doi: 10.3969/j.issn.2095-4344.2014.46.020
    Abstract ( 450 )   PDF (680KB) ( 857 )   Save

    BACKGROUND: Orthodontic tooth movement is based on the periodontal tissue remodeling. In the exogenous factors accelerating orthodontic tooth movement, Chinese herbal medicine has become a research hotspot because of its wide resources, low cost, easy to extract, mild effect, small toxic, less side effects and drug resistance.

    OBJECTIVE: To summarize the role of Chinese herbal medicine in the periodontal tissue remodeling during orthodontic tooth movement.
    METHODS: A computer-based retrieval of CNKI, Wanfang and PubMed databases was performed for articles related to Chinese herbal medicine for improving orthodontic tooth movement published before 2014. The keywords were “Chinese herbal medicine, orthodontic tooth movement, periodontal tissue remodeling” in Chinese and English, respectively.
    RESULTS AND CONCLUSION: Erigeron breviscapus, Salvia, teasel, Drynaria, baicalin, evening primrose oil as Chinese herbs are most widely used in the promotion of periodontal tissue remodeling, characterized as wide resources, low cost, easy to extract, mild effect, low toxicity, less drug resistance. In the clinical orthodontic treatment, it is hoped to accelerate orthodontic tooth movement and shorten the treatment time. Therefore, under the appropriate corrective force, Chinese herbs can be used properly to improve periodontal tissue repair and remodeling, which can improve the microcirculation of periodontal tissue, increase the local blood flow, promote bone formation and repress bone resorption.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Condylar resorption: etiology manifestations and modern restorative treatment
    Li Zhen-xing, Chen Chuan-jun
    2014, 18 (46):  7482-7486.  doi: 10.3969/j.issn.2095-4344.2014.46.021
    Abstract ( 1907 )   PDF (577KB) ( 1446 )   Save

    BACKGROUND: Due to complex etiology, manifestations, symptoms, development and outcomes, there is no article about the detailed introduction of condylar resorption in China.

    OBJECTIVE: To analyze the etiology, pathogenesis, clinical manifestations, diagnosis and treatment of condylar resorption, thereby providing a theoretical basis for clinical treatment of condylar resorption.
    METHODS: An online computer-based retrieval of PubMed database and CNKI database between January 1990 and January 2014 was performed by the first author. The keywords were “temporomandibular joint, condylar resorption, pathogenesis, diagnosis, treatment” in English and Chinese, respectively. Finally 38 literatures on the etiology, clinical presentation, diagnosis and treatment of condylar resorption were included.
    RESULTS AND CONCLUSION: Condylar resorption was subdivided into secondary condylar resorption and primary condylar resorption. Secondary condylar resorption has clear risk factors, including condylar fractures, orthognathic surgery, connective tissue or autoimmune diseases and rheumatoid arthritis. Primary condylar resorption may be associated with lowered serum estradiol concentration. Condylar resorption can be diagnosed by imaging studies combined with clinical manifestations and disease history. Condylar resorption treatment measures mainly include medications, splint treatment, occlusal reconstruction, orthognathic surgery, rib-cartilage transplantation and total joint replacement surgery, in conjunction with orthodontic treatment. Currently, its complex etiology and pathogenesis has not been fully elucidated, and we need to conduct further studies.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Exercise and bone metabolism: evaluation based on bone mineral density, bone biomechanics and biochemical indicators 
    Fu Lei, Ke Dan-dan, Zhang Ling-li, Lu Da-jiang
    2014, 18 (46):  7487-7491.  doi: 10.3969/j.issn.2095-4344.2014.46.022
    Abstract ( 436 )   PDF (628KB) ( 704 )   Save

    BACKGROUND: Based on the blood and urine biochemical changes in different intensities and types of exercise, this research describes the impact of exercise on bone metabolism. Meantime, these biochemical indexes also show a method of marking physiological differences of certain individuals, and also reflect the impact of exercise on bone. These can be regarded as a monitoring index of bone growth and metabolism.

    OBJECTIVE: To study exercise effect from the biochemical indicators in bone metebolism, and make a further discussion about its influence on bone formation and bone resorption.
    METHODS: Databases of CNKI and PubMed were retrieved by computer with key words of “exercise; bone metabolism; biochemical indicators” in Chinese and English, respectively, by screening titles and abstracts to search papers related to exercise effects on the blood and urine biochemical indicators. Totally 236 papers were initially found, and only 38 papers were included in result analysis.
    RESULTS AND CONCLUSION: Low-intensity exercise shows less effect on the bone, but excessive exercise is harmful to the bone that can cause stress fractures. What’s worse, it causes a lack of female hormone and reduce bone mass. Exercise can alter the blood and urine biochemical indicators in aspects of types of exercise and intensities. Only the exercise at appropriate intensity makes positive effects on bone formation and resorption, and assists bone mineral density and bone biomechanical indicators to develop exercise prescription and arrange rehabilitation exercises. MechanismS of bone turnover need further discussion at the cellular level.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Exercise and adenosine triphosphate-sensitive potassium channel 
    Zhang Ru-jiang, Song Yong-jing
    2014, 18 (46):  7492-7497.  doi: 10.3969/j.issn.2095-4344.2014.46.023
    Abstract ( 475 )   PDF (550KB) ( 1075 )   Save

    BACKGROUND: In the condition of exercise physiology, adenosine triphosphate-sensitive potassium (KATP) channel plays an important role in many aspects, such as regulation of coronary artery tension, exercise-induced myocardial protection effect and delay of skeletal muscle fatigue.

    OBJECTIVE: To review and investigate the role of KATP in exercise in order to provide theoretical reference for understanding mechanism underlying exercise regulation of body’s metabolism.
    METHODS: A computer-based online search of PubMed and VIP databases was performed for articles related to molecular structure, biological function and regulatory role of KATP as well as correlation of KATP with coronary artery, myocardium, skeletal muscle fatigue and exercise ability published from January 1991 to June 2014. The keywords were “KATP channels; adenosine triphosphate; sports; myocardium; ion channels” in English and Chinese, respectively. Finally, 42 relevant articles were included in result analysis.
    RESULTS AND CONCLUSION: Coupling with cellular energy metabolism and cell membrane excitability, KATP channel is one of the effectors for myocardial protection in response to various physiological and pathological stresses. Prolonged endurance training can increase the expression of skeletal muscle and myocardial KATP, which may be an adaptive performance of the myocardium and skeletal muscle in response to exercise stress. KATP may participate in regulation of coronary blood flow. Myocardial KATP plays an important protective role for alleviating myocardial ischemia-reperfusion injury induced by exercise. When skeletal muscle fatigue occurs, the activation of KATP is helpful to prevent muscle fiber damage and cell death due to excessive consumption of ATP, in favor for the fast recovery from fatigue. The relation between KATP and exercise ability still needs further research.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Electrical stimulation of the pudendal nerve for neurogenic bladder dysfunction after spinal cord injury: a literature research on functional reconstruction 
    Xie Bin, Yue Yu-shan, Zhu Yi, Wang Jian-wei, Cheng Jie
    2014, 18 (46):  7498-7502.  doi: 10.3969/j.issn.2095-4344.2014.46.024
    Abstract ( 363 )   PDF (610KB) ( 460 )   Save

    BACKGROUND: Stimulation of the pudendal nerve as a target can improve neurogenic bladder dysfunction after spinal cord injury, by adjusting the frequency, intensity, pulse width, as well as regulating synergistic effect of detrusor and urethral sphincter.

    OBJECTIVE: To assess the research status of electrical stimulation of the pudendal nerve in the treatment of neurogenic bladder dysfunction after spinal cord injury.
    METHODS: We searched the following databases for articles addressing electrical stimulation of the pudendal nerve for neurogenic bladder dysfunction after spinal cord injury: PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), China National Knowledge Infrastructure, Wanfang Database, and VIP Database. The search was updated to July 2014, and the reference lists of the identified studies were manually screened for additional studies. The study selection and data extraction were independently conducted by two reviewers.
    RESULTS AND CONCLUSION: Fourteen studies were included in this review. We derived the following points by analyzing the included studies: Electrical stimulation of the pudendal nerve has positive effects on rehabilitation of neurogenic bladder dysfunction after spinal cord injury; pudendal nerve electrical stimulation can modulate the coordination of the detrusor and the external urethral sphincter, improve the bladder compliance and restore bladder function by regulating pudendal-to-bladder reflex and spinal reflexes to the bladder. Electrical stimulation of the pudendal nerve may be potentially implemented as a feasible treatment of neurogenic bladder dysfunction after spinal cord injury. More high quality researches should be conducted to clarify the efficacy and the potential active mechanisms of pudendal nerve electrical stimulation for neurogenic bladder dysfunction after spinal cord injury because the number of clinical reports published in this study area is limited, neurophysiological mechanisms underlying biphasic regulation of frequency on bladder function are not well known, and which intensity of pudendal nerve electrical stimulation is more effective than others for the treatment of neurogenic bladder dysfunction after spinal cord injury remains unknown.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Advances in techniques of hepatic vascular exclusion and construction
    Yang Huan, Wang Zhi-peng, Zhang Jin-hui
    2014, 18 (46):  7503-7508.  doi: 10.3969/j.issn.2095-4344.014.46.025
    Abstract ( 444 )   PDF (647KB) ( 508 )   Save

    BACKGROUND: Hepatic vascular exclusion is important for liver transplantation that can reduce blood loss and make for liver recovery. A variety of favorable hepatic vascular exclusion techniques occur in the development of liver surgery technology, which is still a research hot in surgical study.

    OBJECTIVE: To introduce the latest research and the hot spot of hepatic vascular exclusion techniques.
    METHODS:A computer-based online search of PubMed and Wanfang databases for articles relevant to Pringle maneuve, total hepatic vascular exclusion, selective hepatic vascular exclusion and sectional vascular exclusion under hepatectomy published from January 1999 to January 2014. Totally 50 articles were included in result analysis.
    RESULTS AND CONCLUSION: There are a variety of hepatic vascular exclusion technologies, and intermittent hepatic vascular occlusion and semihepatic vascular exclusion are used most commonly. The applicable principles are as follows: (1) Surgery without vascular exclusion is suitable for < 5 cm lesions at the liver edge. (2) Semi-hepatic vascular exclusion is fit for semi-hepatic lesions, especially for patients accompanied by liver cirrhosis. Hepatic vascular exclusion with preservation of semi-hepatic artery and liver hanging maneuver are also reported to have a certain value in clinical practice still need further studies. (3) Intermittent hepatic vascular exclusion is suitable for lesions over half a liver or spanning liver halves (huge lesions). (4) Total hepatic vascular exclusion and its modified technologies are suitable for lesions involving the inferior vena cava and (or) hepatic vein, or lesions closely related to the second and third porta hepatis. (5) Segmental hepatic vascular exclusion is considered for smaller lesions confined to the liver segment under allowed conditions, but semi-hepatic vascular exclusion and Pringle maneuver can be also considered. Depending on patient’s conditions, to select the appropriate method is the key to reduce bleeding and to ensure patient safety.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Genomics and gene polymorphism of immunosuppressive drugs after kidney transplantation
    Yu Li-chong, Qian Ye-yong, Shi Bing-yi, Fan Yu, Liu Lu-peng, Yu Fei
    2014, 18 (46):  7509-7514.  doi: 10.3969/j.issn.2095-4344.2014.46.026
    Abstract ( 383 )   PDF (620KB) ( 466 )   Save

    BACKGROUND: Wide variation between individuals leads to instability of drug concentration that still troubles transplant recipients. Therefore, individual therapy has always been a hot topic following transplantation.

    OBJECTIVE: To review the research progress in the genomics and gene polymorphism of the main categories of immunosuppressive drugs after kidney transplantation.
    METHODS: A computer-based search of Wanfang and PubMed databases was used to retrieve relevant articles published from January 2005 to August 2014. The keywords were “renal transplantation; immunosuppressant drugs; polymorphism; individual treatment” in Chinese and English, respectively. Finally, 50 articles related to genomics and gene polymorphisms of immunosuppressive drugs after kidney transplantation were enrolled in result analysis.
    RESULTS AND CONCLUSION: Immunosuppressant drugs have been widely used among renal transplant recipients to decrease post-renal transplantation rejection rate and greatly improve the survival rate of renal transplant recipients. Because of its certain side effects and wide variation between individuals, therapeutic drug monitoring should be employed routinely after transplantation to keep blood levels within the therapeutic range. This monitor system is effective to avoid post-renal transplantation rejections and drug side effects to a certain extent. Research on the relationship between pharmacokinetics and pharmacodynamics and genetic factors which combined with therapeutic drug monitoring provides possibility to give specific doses that will improve efficacy while decrease side effects of immunosuppressive drugs, thereby further improving the long-term graft survival rate.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Tendon adhesion prevention: status and progress
    Gao Jun, Wang Wei, Na Lei, Jiang Hong-tao, Liu Shi-bo, Wang Pei
    2014, 18 (46):  7515-7519.  doi: 10.3969/j.issn.2095-4344.2014.46.027
    Abstract ( 577 )   PDF (743KB) ( 999 )   Save

    BACKGROUND: Tendon adhesions after tendon repair have been one of the problems difficult to solve in clinical practice.

    OBJECTIVE: To introduce the current situation and progress of the prevention of postoperative tendon adhesions.
    METHODS: A computer-based search of CNKI, Wanfang, PubMed databases for articles related to prevention of tendon adhesions published from January 1971 to May 2014 using the keywords of “tendon injury; tendon adhesions; progress” in Chinese and English, respectively. Repetitive content and irrelevant articles were excluded, and finally 40 articles were included in result analysis.
    RESULTS AND CONCLUSION: In this paper, we analyze the characteristics of tendons and causes of tendon adhesions, summarize the methods for prevention of tendon adhesions and the therapeutic effects, and thereby analyze the pros and cons of various methods and materials for prevention of tendon adhesions. Prevention of tendon adhesions after tendon repair involves various aspects: thorough debridement, better surgical approach, microsurgical repair of tendon tissues, applications of drugs and barrier materials, physical therapy as well as appropriate functional exercise after tendon repair. The integrated use of these means can be done to prevent the occurrence of postoperative tendon adhesions.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Myofascial trigger points: the common cause of clinical tissue pain 
    Liu Lin, Huang Qiang-min, Tang Li
    2014, 18 (46):  7520-7527.  doi: 10.3969/j.issn.2095-4344.2014.46.028
    Abstract ( 1141 )   PDF (846KB) ( 2451 )   Save

    BACKGROUND: Myofascial trigger points have been widely applied in clinical rehabilitation and tissue pain field in the United States and Europe countries, and they have been recognized as the common cause of clinical musculoskeletal pain, joint function limitation, tissue injuries and muscle fatigue by many physiotherapists abroad. However, in China, many experts still have some mistaken ideas and limitations to understand the pathological mechanism and to diagnosis and treat myofascial trigger points.

    OBJECTIVE: From the aspects of the etiology, pathological mechanism, diagnosis and positioning, treatments, to elaborate the method issues and the clinical experience of treatments of myofascial trigger points.
    METHODS: PubMed, ScienceDirect, EBSCO and CNKI databases were searched by the keywords of “myofascial trigger points, myofascial pain syndrome” in Chinese and English, respectively, in the titles and abstract to retrieve relevant articles published from the time of database construction to August 2014.
    RESULTS AND CONCLUSION: It is concluded that a child has myofascial trigger points in some skeletal muscles after age of 4 years. The main causes of myofascial trigger points include issue trauma, the wrong posture, bone and joint degeneration, nutrition deficiency, mental stress, chronic infection and so on. The pathological mechanism of myofascial trigger points remains unknown, but what has been widely accepted is the integrated trigger point hypothesis introduced by Simons. And how to find and position myofascial trigger points is the key point to treat this disease successfully. The application of myofascial trigger points techniques is important for the rehabilitation of clinical tissue pain and the occurrence and spread of bone and joint injuries, myofascitis, muscle pain, muscle fatigue and so on.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effect of sodium hyaluronate on malondialdehyde and superoxide dismutase levels in the synovial fluid of patients with knee osteoarthritis
    Yu Qing-bo, Deng Jian-feng, Gao Da-xin, Song Hong, Yan Jia-liang, Qi Xi-ming, Wang Dong-xing
    2014, 18 (46):  7528-7532.  doi: 10.3969/j.issn.2095-4344.2014.46.029
    Abstract ( 350 )   PDF (595KB) ( 508 )   Save

    BACKGROUND: Intra-articular injection of sodium hyaluronate is an effective method for the treatment of knee osteoarthritis, with significant effect and less adverse reactions, but the mechanism is unclear.

    OBJECTIVE: Through testing the malondialdehyde and superoxide dismutase levels in the synovial fluid of knee osteoarthritis before and after injection of sodium hyaluronate, to evaluate the clinical efficacy of sodium hyaluronate in the treatment of knee osteoarthritis.
    METHODS: Thirty-seven patients with knee osteoarthritis (40 knees) were enrolled and divided into mild (n=10, 10 knees), moderate (n=17, 18 knees), and severe (n=10, 12 knees) groups according to the Japan's knee osteoarthritis indications. Patients were subjected to intra-articular injection of 25 mg sodium hyaluronate, once a week for 5 weeks. The levels of malondialdehyde and superoxide dismutase in the synovial fluid before and 4 weeks after treatment were detected, and then clinical effects were evaluated based on the clinical scores according to the Japan’s knee osteoarthritis indications.
    RESULTS AND CONCLUSION: The indication rating results of the mild and moderate groups were decreased significantly 4 weeks after injection (P < 0.05), but there were no significant difference in the severe group before and after treatment. The malondialdehyde level in the synovial fluid was decreased obviously in the three groups at 4 weeks after injection (P < 0.05), while the level of superoxide dismutase was increased remarkably (P < 0.05). These findings indicate that sodium hyaluronate can treat knee osteoarthritis by reducing the malondialdehyde level and increasing superoxide dismutase level in the synovial fluid, but this method is more suitable for treatment of mild to moderate knee osteoarthritis.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Relationship of serum estradiol levels with age and lesion severity in women with knee osteoarthritis  
    Liang Hai-bo, Su Wei, Luo Shi-xing
    2014, 18 (46):  7533-7537.  doi: 10.3969/j.issn.2095-4344.2014.46.030
    Abstract ( 290 )   PDF (648KB) ( 498 )   Save

    BACKGROUND: The pathogenesis of knee osteoarthritis is still not very clear, and there is a lack of early diagnosis methods. Because it is more common in middle-aged women, it is suggested that the pathogenesis of knee osteoarthritis may be closely related to serum estradiol levels in the human body.

    OBJECTIVE: To explore the correlation between the level of serum estradiol and the severity of osteoarthritis in female patients with knee osteoarthritis.
    METHODS: Seventy-six female patients with knee osteoarthritis admitted in the First Affiliated Hospital of Guangxi Medical University from February 2012 to December 2013 were enrolled, and divided into premenopause group (n=42) and postmenopause group (n=34). According to Kellgren-Lawrence (K-L) grading, both two groups were subdivided into K-L 0, II and IV groups. The knee function was evaluated with Hospital for Special Surgery (HSS) Knee Score Standard in USA, the serum estradiol levels were detected with electrochemiluminescence immunoassay. Then, the correlation analysis was performed.
    RESULTS AND CONCLUSION: The serum estradiol levels of the premenopause group and postmenopause group were decreased significantly with the increase of K-L grade and age (P < 0.05). Under the same K-L grade, the serum estradiol levels in the postmenopausal patients were significantly lower than those in the premenopausal patients. Linear regression analysis showed that there was a negative relationship between age and serum estradiol level (R2=0.222, P < 0.05), and there was a positive correlation between HSS scores and serum estradiol levels (R2=0.415, P < 0.05). The severity of knee osteoarthritis in female patients shows a negative correlation with the level of serum estradiol but a positive correlation with age.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Subacromial impingement syndrome: a systematic review of arthroscopic versus open subacromial decompression  
    Dong Yan, Zhang Ke-yuan, Sun Xue-bin, Li Gang, Nijiati Abulimiti, Liu Yang
    2014, 18 (46):  7538-7544.  doi: 10.3969/j.issn.2095-4344.2014.46.031
    Abstract ( 418 )   PDF (849KB) ( 400 )   Save

    BACKGROUND: It is still disputed about the treatment of subacromial impingement syndrome at present, arthroscopic subacromial decompression or open subacromial decompression.

    OBJECTIVE: To assess the clinical therapeutic effects of arthroscopic subacromial decompression versus open subacromial decompression in the treatment of subacromial impingement syndrome.
    METHODS: Controlled trials on arthroscopic subacromial decompression versus open acromioplasty in the treatment of subacromial impingement syndrome were collected in different databases through computer search. There was no limitation for language, randomized controlled trials or blinded studies. At last, the meta-analysis was conducted by using RevMan5.0 software.
    RESULTS AND CONCLUSION: Four randomized controlled trials and six non-randomized controlled trials were included in this study, involving 583 patients with subacromial impingement syndrome. The results of meta-analysis prompted that as to the hospital inpatient days and time until return to work, the group of arthroscopic subacromial decompression was shorter than open subacromial decompression. However, as to the postoperative shoulder functional scores, the operative time, patient’s satisfaction, and the rate of excellent and good results of postoperative UCLAs, there were no significant statistical differences between two groups. Due to the certain limitations and deficiencies of the quantity and quality in the included trials, there must still need large-sample, multi-centered, high-quality randomized controlled trials to confirm these results.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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