Changes of cartilage matrix components, related growth factors and inflammatory factors in the temporomandibular joint under high acceleration

doi:10.3969/j.issn.2095-4344.2015.11.005

Abstract

Abstract:

BACKGROUND: Under repeated +Gz condition from the strong acceleration, small traumas resulting from pressures and impacts in various directions are formed inside the temporomandibular joint, thereby leading to temporomandibular joint disorders.

OBJECTIVE: To investigate the changes in collagen type II, proteoglycans, collagenase, insulin-like growth factor-1, transforming growth factor-β1, tumor necrosis factor-α, interleukin-2 and interleukin-3 in the cartilage matrix after temporomandibular joint disorder.

METHODS: Thirty-six rats were randomly divided into three groups: negative control group, only fixed on the centrifugal machine for 5 minutes, five consecutive times per day, 4 days per week, totally for 3 weeks; +5 Gz group, centrifugation at +5 Gz for 5 minutes, five consecutive times per day, 4 days per week, totally for 3

weeks; +10 Gz group, centrifugation at +10 Gz for 5 minutes, five consecutive times per day, 4 days per week, totally for 3 weeks. All animals were on an empty stomach for 12 hours prior to daily centrifugation. Chondrocytes from the temporomandibular tissue were isolated and cultured in vitro to extract total protein that was detected by weste blot assay.

RESULTS AND CONCLUSION: Compared with the negative control group, the levels of collagen type II, proteoglycans, collagenase, insulin-like growth factor-1, transforming growth factor-β1 in the cartilage matrix after temporomandibular joint were significantly decreased, but the levels of tumor necrosis factor-α, interleukin-2 and interleukin-3 were significantly increased under repeated +Gz conditions. These findings indicate that under repeated +Gz stresses, a great damage to the cartilage matrix of the temporomandibular joint is found as well as reduced repair capability and increased inflammatory reactions.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Collagen Type II, Insulin-Like Growth Factor I, Transforming Growth Factor beta1, Tumor Necrosis Factor-alpha, Interleukin-2, Interleukin-3

CLC Number:

R318

Li Hong-shi, Chen Xin, Feng Yan, Yin Yin, Cao Jun-kai. Changes of cartilage matrix components, related growth factors and inflammatory factors in the temporomandibular joint under high acceleration [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1663-1667.

Figures/Tables 1

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