Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2015.11.006

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (11): 1668-1672.doi: 10.3969/j.issn.2095-4344.2015.11.006

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Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells

Liu Jun¹, Hu Bo¹, Jiang Xin-yi², Sun Ji-cheng¹, Deng Feng¹, Song Jin-lin¹

¹The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China; ²Department of Stomatology, the Center Hospital of Mianyang, Mianyang 62100, Sichuan Province, China

Revised:2014-12-25 Online:2015-03-12 Published:2015-03-12
Contact: Song Jin-lin, M.D., Professor, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China
About author:Liu Jun, Studying for master’s degree, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China
Supported by:
the National Natural Science Foundation of China, No. 30870754

Abstract

Abstract:

BACKGROUND: Both of low-intensity pulsed ultrasound (LIPUS) and bone morphogenetic protein-2 (BMP-2) can promote the osteogenic differentiation of human periodontal ligament cells, but there is no report on their combination application.

OBJECTIVE: To evaluate the biological effect of LIPUS combined with BMP-2 on osteogenic differentiation of human periodontal ligament cells.

METHODS: Human periodontal ligament cells were isolated, cultured, passaged and identified. Passage 4 cells were seeded onto 6-well plates and divided into four groups: control group, LIPUS group, BMP-2 group, LIPUS+BMP-2 group. Alkaline phosphatase activity was detected by alkaline phosphatase activity measurement kit, and the expression of type I collagen and osteopontin was detected by RT-PCR.

RESULTS AND CONCLUSION: After LIPUS, BMP-2, and their combination treatment, the activity of alkaline phosphatase was significantly higher than that in the control group (P < 0.05), and the activity of alkaline phosphatase was highest in the LIPUS+BMP-2 group (P < 0.05). RT-PCR results showed that both of LIPUS and BMP-2 could augment the mRNA expression of type I collagen and osteopontin (P < 0.01), but it was more distinct when they were used together (P < 0.001). In a conclusion, the combined utilization of LIPUS and BMP-2 shows stronger ability in promoting the osteogenic differentiation of human periodontal ligament cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Sonication, Bone Morphogenetic Proteins, Periodontal Ligament

CLC Number:

R318

Liu Jun, Hu Bo, Jiang Xin-yi, Sun Ji-cheng, Deng Feng, Song Jin-lin. Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1668-1672.

Figures/Tables 2

References

[1] Mi HW, Lee MC, Fu E, et al. Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer. Gene Ther.2011;18(5):452-461.
[2] Pitaru S, McCulloch CA, Narayanan SA. Cellular origins anddifferentiation control mechanisms during periodontal development and wound healing. J Periodontal Res. 1994; 29(2): 81-94.
[3] Iwata T, Yamato M, Ishikawa I, et al. Tissue engineering in periodontal tissue. Anat Rec.2014;297(1):16-25.
[4] Hu B,Zhang Y,Zhou J,et al.Low-Intensity Pulsed Ultrasound Stimulation Facilitates Osteogenic Differentiation of Human Periodontal Ligament Cells. PLoS ONE.2014;9(4):e95168.
[5] E lingling, Xu lulu, Wu xia, et al.The interactions between rASCs, rhBMP-2 and β-TCP play an important role in bone tissue engineering. Tissue Engineering Part A.2010;16(9):2927-2940.
[6] Ferrandis E,Pradhananga S,Touvay C,et al.Immunogenicity, toxicology, pharmacokinetics and pharmacodynamics of growth hormone ligand-receptor fusions. Clin Sci.2010; 119(11):483-491.
[7] Rego EB,Takata T,Tanne K,et al.Current Status of Low Intensity Pulsed Ultrasound for Dental Purposes. Open Dent J .2012;6:220-225.
[8] Romano CL, Romano D, Logoluso N . Low-intensity pulsed ultrasound for the treatment of bone delayed union or nonunion: a review. Ultrasound Med Biol.2009; 35(4):529-536.
[9] Ikai H, Tamura T, Watanabe T, et al. Low-intensity pulsed ultrasound accelerates periodontal wound healing after flap surgery. J Periodontal Res.2008;43(2):212-216.
[10] 高翔,宋锦璘,邓锋,等.低强度脉冲超声波联合引导骨再生术对Beagle犬牙周骨开窗缺损修复效应的研究[J].中国生物医学工程学报,2012,31(6):895-901.
[11] Zhao M,Xiao G,Berry JE,et al. Bone morphogenetic protein 2 induces dental follicle cells to differentiate toward a cementoblast/osteoblast phenotype. J Bone Miner Res. 2002; 17(8):1441-1451．
[12] Ren L, Yang Z, Song J, et al. Involvement of p38 MAPK pathway in low intensity pulsed ultrasound induced osteogenic differentiation of human periodontal ligament cells. Ultrasonics.2013;539(3):686-690.

Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells

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