Bone mesenchymal stem cells with allogeneic bone to repair canine mandibular defects: detection of osteogenic ability

doi:10.3969/j.issn.2095-4344.2015.14.002

Abstract

Abstract:

BACKGROUND: Allogeneic bone has anatomical appearance and biological features similar to autogenous bone, which is an excellent biological scaffold material. Mesenchymal stem cells originating from autogenous bone marrow have mutli-lineage differentiation potential, can differentiate into osteoblasts and chondrocyte, and thus can accelerate the formation of bone tissue and cartilage tissue.

OBJECTIVE: To establish the osteogenic ability of allogeneic bone with autogenous bone marrow mesenchymal stem cells for repairing major mandibular defects.

METHODS: The left mandibular teeth of 24 beagles were extracted, and at 2 months after wound healing, mandibular defects were made artificially. The beagles were divided into two groups: control group treated with lyophilized allogeneic bone, and experimental group with autogenous bone marrow mesenchymal stem cells and lyophilized allogeneic bone. Densitometry with CT and Micro-CT was conducted 4, 12, and 24 weeks after surgery.

RESULTS AND CONCLUSION: Compared with the control group, the bone density of the mandible was significantly higher in the experimental group at 12 weeks after transplantation (P < 0.05). Over time, the bone densities in the two groups were both increased, but the bone density in the experimental group was always higher than that in the control group. Bone structure parameters were progressively increased or decreased in the two groups, especially in the experimental group. At 24 weeks after surgery, the degree of trabecular separation in regions of interest was higher in the experimental group than the control group (P < 0.05), but the bone volume fraction, number of trabecular bone, and bone trabecular thickness were significantly lower in the experimental group than the control group (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells are capable of accelerating the reconstruction of allogeneic bones.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Mandible, Bone Remodeling, Transplantation, Homologous

CLC Number:

R394.2

Jing Cai-xia, Liu Chang-kui, Tan Xin-ying, Luo Jin-chao, Hu Min. Bone mesenchymal stem cells with allogeneic bone to repair canine mandibular defects: detection of osteogenic ability [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2138-2143.

Figures/Tables 5

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