Platelet-rich plasma accelerates the proliferation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.14.003

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma contains a variety of stimulating factors, and can also raise the proteoglycan and collagen synthesis.

OBJECTIVE: To observe the effect of platelet-rich plasma on the proliferation of goat bone marrow mesenchymal stem cells.

METHODS: Blood samples were extracted from the jugular vein of Inner Mongolia Ximeng goats to harvest platelet-rich plasma using centrifugation method. Then, bone marrow was extracted from the goat’s ilium by puncture method to isolate and purify goat bone marrow mesechymal stem cells using density gradient centrifugation method. After that, primary cells at good state were cultured in L-DMEM complete medium containing 10%, 20%, 30% platelet-rich plasma or in simple L-DMEM complete medium.

RESULTS AND CONCLUSION: Within 2-6 days of culture, cells in the platelet-rich plasma groups proliferated faster than those in the control group, and with the increasing of platelet-rich plasma concentration, the cells grew faster, with larger number and more mature morphology. At 4 days of culture, the cell doubling time was about 50, 35, 25 hours in the 10%, 20%, and 30% platelet-rich plasma groups, respectively. These findings indicate that goat platelet-rich plasma can dramatically promote the proliferation of bone marrow mesenchymal stem cells in a concentration-dependent manner.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Platelet-Rich Plasma, Cell Proliferation, Goats

CLC Number:

R394.2

Xu Xiang, Yin He-ping. Platelet-rich plasma accelerates the proliferation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2144-2148.

Figures/Tables 3

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