Effects of Jiegu Qili Tablet on proliferation and mineralization of MC-3T3 cells

doi:10.3969/j.issn.2095-4344.2015.11.011

Abstract

Abstract:

BACKGROUND: Jiegu Qili Tablet is a compound Chinese medicine which can promote fracture healing, but its mechanism is still unclear.

OBJECTIVE: To observe the effects of Jiegu Qili Tablet on the proliferation and mineralization of MC-3T3 cells.

METHODS: MC-3T3 cells cultured in vitro were selected. (1) After 24 hours of routine culture, cells were cultured in serum-free DMEM for another 24 hours to synchronize cell cycles. Then, the culture solution was removed, and cells were cultured in DMEM complete medium with (experimental group) or without (control group) Jiegu Qili Tablet solution. At 24 and 48 hours of culture, MTS assay was used to detect the proliferative ability of cells. (2) In the experimental group, MC3T3-E1 cells at a density of 1×10⁵ were inoculated into 96-well plates, and after 4 hours of culture, cells were cultured in an osteogenic induction medium. After 21 days of osteogenic induction, the mineralization of MC3T3-E1 cells was examined using alizarin red S staining. Cells in the control groups were treated without osteogenic induction.

RESULTS AND CONCLUSION: Compared with the control group, the cell proliferation rate was up-regulated in the experimental group at 24 and 48 hours after culture (P < 0.05), and the number of calcium nodules was also larger in the experimental group at 21 days after osteogenic induction (P < 0.01). These findings indicate that Jiegu Qili Tablet solution can up-regulate the proliferation and mineralization of MC-3T3 cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Osteoblasts, Cell Proliferation, Fracture Healing, Drugs, Chinese Herbal

CLC Number:

R318

Yao Ming-zhi. Effects of Jiegu Qili Tablet on proliferation and mineralization of MC-3T3 cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1694-1698.

Figures/Tables 2

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