Effect of nux vomica on fracture healing in rabbits

doi:10.3969/j.issn.2095-4344.2015.11.002

Abstract

Abstract:

BACKGROUND: The main active ingredient of nux vomica is total alkaloids that can activate meridian to stop pain, reduce swelling, excite stomach and cool blood, and also have some pharmacological effects on the nervous system and cardiovascular system.

OBJECTIVE: To investigate the effects of nux vomica on the fracture healing process of experimental rabbits.

METHODS: Rabbit models of radius fracture were established and then randomized into drug administration group and control group, with 10 animals in each group. At room temperature, the nux vomica powder mixed suspension was administrated into the rabbits in the drug administration group, while the normal saline was administrated into the rabbits in the control group, once a day. X-ray examination was performed at 7, 14, 28 days after administration to compare the fracture healing between the two groups. Blood samples from ear vein were taken at 14 and 28 days after drug administration for serological comparison to analyze the concentration changes of serum alkaline phosphatase and trace elements including Ca, Mg, Cu, Fe, Zn.

RESULTS AND CONCLUSION: Rabbit bone X-ray examinations results showed that compared with the control group, the fracture healing of the drug administration group was faster. The concentration of serum alkaline phosphatase of rabbits in the drug administration group at 14 days and 28 days after drug administration were higher than that before drug administration and in the control group at the same time respectively (P < 0.05). The serum Fe concentration of rabbits in the control group at 28 days after normal saline administration was higher than that before normal saline administration (P < 0.05). The serum Ca and Zn levels of rabbits in the drug administration group at 14 days and 28 days after drug administration were higher than those before drug administration and the control group at the same time, respectively (P < 0.05). The Mg and Fe levels of rabbits in the drug administration group at 14 days after drug administration were significantly higher than those before drug administration and those in the control group at the same time, respectively (P < 0.01, P < 0.05). The Fe level of rabbits in the drug administration group at 28 days after drug administration was significantly higher than that before drug administration (P < 0.01). Nux vomica can increase the alkaline phosphatase and trace elements levels, and play a promoting role in the process of healing.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Drugs, Chinese Herbal, Fractures, Bone, Alkaline Phosphatase

CLC Number:

R318

Li Chang-lei, Ma Bao-miao, Liu Wei, Chen Xiao-qing, Yi Hui-ling, Shu Xi-ji. Effect of nux vomica on fracture healing in rabbits [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1647-1651.

Figures/Tables 4

2.1 实验动物数量分析实验造模选用家兔为20只，没有脱失，均进入结果分析。 2.2 家兔骨折愈合情况给药后7，14，28 d X射线影像学检查结果见图1-3。从图1可知：给药后7 d，给药组家兔X射线影像学结果显示骨折断端呈完全分离状态，无明显位移，骨折线明显，骨折部位周围组织肿胀明显，有少量骨痂形成。对照组家兔骨折断端呈完全分离状态，无明显位移，骨折线明显，骨折部位周围组织肿胀明显，但无骨痂形成。从图2可知：给药后14 d，给药组家兔X射线影像学结果显示骨折处有较多骨痂生成，间隙密度增大；骨折断端逐渐模糊，已有部分骨折线较紧密的连接在一起；骨折部位周围组织肿胀逐渐消退。对照组家兔骨折处有较少量骨痂生成，间隙依旧较大，骨折线较清晰；骨折部位周围组织肿胀现象仍较明显。从图3可知：给药后28 d，给药组家兔X射线影像学结果显示骨折处骨痂几乎完全吸收，皮质密度接近正常皮质骨；骨折线消失；骨髓腔复原；骨折部位周围组织肿胀现象消失；骨折基本痊愈。对照组家兔骨折处虽然骨痂生成量增多，但间隙仍然存在，只有较少部分骨折线的连接在一起，骨折线较明显；骨折部位周围组织肿胀现象并未完全消失。 2.3 家兔血清碱性磷酸酶(ALP)，微量元素钙(Ca)、镁(Mg)、铜(Cu)、铁(Fe)、锌(Zn)质量浓度变化结果见表1和表2。从表1可知，随着给药时间的增加，对照组家兔血清中碱性磷酸酶浓度呈先升后降趋势，各时间点相比较于给药前，浓度差异较小，差异无显著性意义(P > 0.05)；给药组家兔血清中碱性磷酸酶浓度呈不断上升趋势。14 d时相比较于给药前和同时间点的对照组，浓度差异较大，差异有显著性意义(P < 0.05)；28 d时相比较于给药前和同时间点的对照组，浓度差异大，差异有显著性意义(P < 0.01)。"

References

[1] 过振华,马红梅,经伯礼.马钱子药理毒理研究回顾及安全性研究展望[J].中西医结合学报,2008,6(6):645-649．
[2] 朱建伟,武继彪,李成韶,等.马钱子碱镇痛作用及其药效动力学研究[J].中国中医药科技,2005,12(3):166-167．
[3] 张娟,肖鲁伟,戴体俊,等.马钱子碱中枢镇痛作用的研究[J]．中国中医药科技,2009,16(1):34-35．
[4] 陈晓莉,李永吉,王录娜,等.HPLC法测定马钱子缓释片中士的宁与马钱子碱[J].哈尔滨商业大学学报:自然科学版,2013,29(4): 390-393.
[5] 李仙仙,魏武.马钱子碱抗肿瘤及减毒增效的研究进展[J].中国医药指南,2013,11(20):486-488.
[6] 中华人民共和国科学技术部.关于善待实验动物的指导性意见[Z].2006-09-30.
[7] 龚飞鹏,于欢,朱龙涛,等.砂烫法与烘烤法对马钱子中抗炎镇痛成分影响[J].江西中医学院学报,2013,25(6):52-53.
[8] 李长雷,马宝苗,陈晓青,等.利用骨科电锯建立兔桡骨骨折模型的研究[J].长江大学学报:自科版,2014,11(27):6-9.
[9] 陈文,祁开泽.中药对骨折愈合影响的研究进展[J].湖南中医杂志, 2010,26(3):17-128.
[10] 陈立涛,李洪波.中医药对骨折愈合干预作用的研究近况[J].实用中西医结合临床,2007,4(7):91-92.
[11] 刘钟华,杨世荣,回春,等.中药促进骨折愈合的实验研究概况[J].长春中医学院学报,2004,20(2):63-65.
[12] 赵利民中,袁惠中,徐恒卫,等.马钱子碱及其中药复方制剂神农丸的抗炎镇痛作用研究[J].中华中医药学刊,2012,30(8): 1874-1876.
[13] 王琦玮,刘良,黄光照.马钱子的毒理学研究进展[J].法医学杂志, 2004,8(3):183-184．
[14] 覃旭,易洪诚.马钱子促进骨折愈合的研究进展[J]．贵阳中医学院学报,2007,29(2):66-68.
[15] 陶玉杰.简述民族医对马钱子的炮制及应用[J]. 中国民族民间医药,2010,19(19):6,8.
[16] 侯晓峰,李朝旭,刘景生.鹿茸对实验型骨折愈合中BMP-2表达的影响[J].辽宁中医杂志,2005,32(4):374-376．
[17] Kloen P,Doty SB,Gordon E,et al. Expression and activation ofthe BMP-signaling components in human fracture nonunions. Bone Joint surg Am.2002;84:1909-1918.
[18] 姚建锋,许鹏,李毅,等.老年髋部骨折患者血清中宏量及微量元素含量分析[J].广东微量元素科学,2007,14(2):13-16.
[19] 吕德成,吴汝舟,姜长明,等.微量元素对家兔骨折愈合影响的实验研究[J].中华骨科杂志,1992,12(4):282-286.
[20] 张奋耿,凌秋英,叶泽亮,等.伤科接骨片对实验性家兔骨折模型血清微量元素浓度的影响[J].中医正骨,2008,20(5):9-10.
[21] 苟峰,沈艳华,孙冰峰.急性重症马钱子中毒抢救治疗成功一例报告[J].右江医学,2014,42(2):246-247.
[22] 青军.口服麝香接骨胶囊致马钱子蓄积中毒1例[J].内蒙古中医药, 2012,31(14):180.
[23] 和平.马钱子中毒1例[J].临床医学,2010,30(7):103-103.
[24] 刘莲芳,肖霞.马钱子中毒抢救成功1例的护理体会[J].现代中西医结合杂志,2006,15(5):651-652.
[25] 林武俊,陈小玉,刘可福.HPLC法测定跳骨片中士的宁和马钱子碱的含量[J].中国保健营养:中旬刊,2014,24(4)2418.
[26] 夏克春,张梅,李平.马钱子碱经皮给药对乳腺癌骨转移抑制机制的探讨[J].中华肿瘤防治杂志,2012,19卷23期 1771-1775.
[27] 金翔,周信,马勇.马钱子在骨伤科的应用[J].中国实验方剂学杂志, 2010,16(7):198-200.
[28] 孙杰.疗伤接骨散治骨折[J].中国民间疗法,2010,18(5):25.
[29] 王元,王波,胡敏,等.龙发伤科七味片研究进展[J].云南中医中药杂志,2010,31(8): 76-77.
[30] 王丹丹.马钱子质量控制及口服给药药物代谢动力学研究[D].南京中医药大学，2009.
[31] 郝吉雪,郭进,陈奇斌.伤科接骨片治疗骨质疏松性骨折的研究[J].河北中医药学报,2008,23(2):20-21.
[32] 宫丽,张继平.伤科接骨片促进骨折愈合研究进展[J].中国中医药信息杂志,2006,13(5):92-94.
[33] 姜卫军,高新洛.中西医结合治疗锁骨骨折85例[J].陕西中医, 2004, 25(8):718-719.
[34] 孙昌宏,王宏强.鹿角霜疗骨折[J].中医杂志,2003,44(5):334.
[35] 廉帼.生马钱子浸渍外洗治疗骨性关节病[J].河南中医,2002, 22(5):63.
[36] 曾小平,温道高.外敷正骨油膏治疗闭合性骨折[J].中国骨伤，2001,14(2):126.