Effects of different doses of puerarin on osteoblasts in vitro

doi:10.3969/j.issn.2095-4344.2015.11.004

Abstract

Abstract:

BACKGROUND: Studies have reported that puerarin can reduce bone resorption and promote bone formation.

OBJECTIVE: To further explore the effects of puerarin at different doses on the proliferation of osteoblasts cultured in vitro．

METHODS: Osteoblasts isolated from the cranium of newborn rats were cultured in vitro, and then passage 3 cells were cultured in DMEM medium with the presence of 0, 20, 40, 80 and 100 µmol/L puerarin+10% fetal bovine serum for 48 hours. The viability of osteoblasts, alkaline phosphatase activity and mineral node formation were determined using MTT, alkaline phosphatase kit and alizarin red staining, respectively.

RESULTS AND CONCLUSION: The viability of osteoblasts treated with puerarin at either 40 or 80 μmol/L was significantly higher than that of the control group. Alkaline phosphatase activity and the number of mineral modules were significantly increased in osteoblasts cultured with puerarin at 40 or 80 μmol/L when compared with the untreated cells. These findings demonstrate that puerarin is able to promote osteoblast proliferation in vitro.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Drugs, Chinese Herbal, Osteoblasts, Alkaline Phosphatase, Cell Proliferation

CLC Number:

R318

Lin Shu-zhong, Liu Jun. Effects of different doses of puerarin on osteoblasts in vitro [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1658-1662.

Figures/Tables 2

References

[1] Kneser U, Schaefer DJ, Polykandriotis E,et al. Tissue engineering of bone: the reconstructive surgeon's point of view. J Cell Mol Med.2006;10:7-19.
[2] Smith LA, Liu XH, Ma PX. Nano-fibrous scaffolds for tissue engineering. Soft Matter. 2008;4:2144-2149.
[3] Wu HQ, Guo HN, Wang HQ, et al. Protective effect and mechanism of puerarin on learning-memory disorder after global cerebral ischemiareperfusion injury in rats. Chin J Integr Med.2009;15:54-59.
[4] Zhou F,Wang L,Liu PP,et al.Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.Neural Regen Res. 2014;9(23): 2074-2080.
[5] Liu XJ,Mei ZG,Qian JP,et al.Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway.Neural Regen Res. 2013;8(34): 3203-3215.
[6] Wang XX, Wu J, Chiba H, et al. Puerariae radix prevents bone loss in ovariectomized mice.J Bone Miner Metab. 2003; 21:268-275.
[7] Blarir HC, Jordan SE, Peterson TG, et al. Variable effects of ty-rosine kinase inhibitor on avian osteoclastic activity and reduction of bone loss in ovariectiomized rats. J Cell Biochem. 1996;61:629-637.
[8] Langer R,Vacanti JP.Tissue engineering. Science.1993;260: 920-926.
[9] Habibovic P, Yuan H, van der Valk CM, et al. 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials.2005;26:3565-3575.
[10] Viereck V, Gründker C, Blaschke S, et al. Phytoestrogen genistein stimulates the production of osteoprotegerin by human trabecular osteoblasts. J Cell Biochem 2002;84: 725-735.
[11] Liu XJ, Mei ZG, Qian JP,et al. Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway. Neural Regene Res.2013;8(34): 3203-3215.
[12] 黄彤,金邦荃,孙桂菊,等.葛根素对去卵巢大鼠骨密度和骨强度的改善[J].现代预防医学,2010,37(20):3894-3896
[13] 李海,金花,黄海玲,等.葛根素对去卵巢大鼠和铅中毒大鼠骨组织的影响[J].现代预防医学,2011,38(18):3742-3744.
[14] 詹秀琴,钱康琦,孙玉明.葛根素对MC3T3-E1细胞TGF-β1 Smad信号通路的作用[J].中成药,2013,35(6):1121-1124.
[15] 王海珍,负云飞,苗志勃.葛根素对大鼠成骨细胞增殖影响的体外实验研究[J].中医药导报,2013,19(3):77-79.
[16] 钱康琦,孙玉明,詹秀琴.蛋白免疫印迹杂交法研究葛根素对成骨细胞TGF-β1及Smad2/3蛋白表达的影响[J].长春中医药大学学报,2013,29(1):18-20.
[17] Liang J, Chen H, Pan W, et al.Puerarin inhibitrs caspasa-3 expression in osteoblasts of diabetic rats.Medicine RePort. 2012;5(6):1419-1422.
[18] 吴琳琳,王彦,郑雅心,等.葛根素对人牙周膜干细胞成骨分化的作用[J].中国美容医学,2013,22(10):1067-1071.
[19] 梁恒,邝志强,王攀攀,等.葛根素通过BMp-2调节去卵巢大鼠骨代谢[J].广州医学院学报,2012,40(3):1-4.
[20] 袁斯远,孔蓓蓓,盛彤,等.葛根素干预小鼠成骨细胞分化相关特征性蛋白mRNA的表达[J].中国组织工程研究, 2014,18(42): 6733-6736
[21] Notoya M, Tsukamoto Y, Nishimura H, et al. Quercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro.Eur J Pharmacol. 2004; 485:89-96.
[22] Garcia T,Roman S,Jackson A,et al.Behavior of osteoblast, adipocyte, and myoblast markers in genome-wide expression analysis of mouse calvaria primary osteoblasts in vitro. Bone. 2002;31:205-211.
[23] Whyte MP. Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocr Rev. 1994;15: 439-461.
[24] 王昌,唐旭磊,陈克明,等,葛根素在体外对成骨细胞增殖和分化的影响[J].中国组织工程研究,2012,16(33):6102-6106.
[25] Maenoa S, Nikia Y, Matsumotoa H, et al.The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials. 2005; 26:4847-4855.