Effects of alpha-zearalanol on the osteogenic differentiation of mouse bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.06.003

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into osteoblasts, and α-zearalanol can promote the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.

OBJECTIVE: To observe the effects of α-zearalanol on the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.

METHODS: Mouse bone marrow mesenchymal stem cells were isolated through whole bone marrow adherence method. Passage 3 cells were divided into non-induced group (cultured by the Iscove’s modified Dulbecco’s medium containing 10% fetal bovine serum and 1% penicillin and streptomycin), blank induced group (induced by the classic osteoblast-induced system with 0.1 μmol/L dexamethasone, 10 mmol/L β-glycerine sodium, and 50 μmol/L vitamin C), positive control induced group (induced by the classic osteoblast-induced system and 10^-⁸ mol/L oestrogen), and low-, median-, high-concentration α-zearalanol groups (induced by the classic osteoblast-induced system and 10^-⁷, 10^-⁶, 10^-⁵ mol/L α-zearalanol). The morphological changes of induced cells were observed by phase contrast microscopy, and the growth curves of cultured cells were plotted based on MTT results. The expression of alkaline phosphatase and osteocalcin were detected by ELISA.

RESULTS AND CONCLUSION: In the non-induced group, cells showed long fusiform shape mostly, and contact inhibition was observed while the cells were close-packed. After induced, cells proliferated effectively, showed triangle, astero-form, polygon or irregular shape, and clumps and multilayer of cells became evident. Compared to both the blank induced group, the alkaline phosphatase activity and osteocalcin expression were significantly lower in the non-induced group, but higher in the positive control induced group and low concentration α-zearalanol group (P < 0.01). These findings indicate that α-zearalanol can promote the proliferation of bone marrow mesenchymal stem cells, and enhance the expression of alkaline phosphatase and osteocalcin expression, especially in the low concentration group. This demonstrates that low concentration α-zearalanol obviously enhances the osteogenic differentiation of mouse bone marrow mesenchymal stem cells in vitro. Up-regulating the release of alkaline phosphatase and osteocalcin may be one of the mechanisms by which α-zearalanol promotes osteogenic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, phytoestrogens, cell differentiation, alkaline phosphatase, osteocalcin

CLC Number:

R394.2

Zou Bin, Zong Shao-hui, Zeng Gao-feng, Fang Ye, Gao Tai-hang. Effects of alpha-zearalanol on the osteogenic differentiation of mouse bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 835-840.

Figures/Tables 2

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