Expression pattern of ten-eleven translocation family during differentiation of human dental pulp cells

doi:10.3969/j.issn.2095-4344.2015.14.022

Abstract

Abstract:

BACKGROUND: Ten-eleven translocation (TET) family proteins are recently discovered DNA dioxygenases that convert methylcytosine to hydroxymethyl cytosine, which is essential for regulating cell proliferation and differentiation, but the expression pattern of TET family proteins in human dental pulp cells is still unclear.

OBJECTIVE: To investigate the expression pattern of TET family proteins during the differentiation of human dental pulp cells.

METHODS: Cellular distribution and expression of TET family proteins were determined by immunofluorescence in human dental pulp cells that were cultured and isolated using digestion method. The protein levels of TETs during cell passage (P1-P7) were detected with western blot assay, and their potential changes during odontogenic induction (7 and 14 days) were confirmed using real-time quantitative PCR and western blot analyses at mRNA and protein levels, respectively.

RESULTS AND CONCLUSION: All TETs were expressed in the nucleus and the cytoplasm of human dental pulp cells During serial cell passage, TET1 protein expression was increased until the 6th passage, TET2 significantly increased at the 2^nd and 3^rd passages and then decreased (P < 0.05), and TET3 showed no statistically significant change (P > 0.05). Both mRNA and protein expression levels of all TETs were elevated during odontogenic induction (P < 0.05). These results indicated that TETs may contribute to cell differentiation of human dental pulp cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Stem Cells, Dental Pulp, Cell Differentiation, Tooth Remineralization

CLC Number:

R394.2

Rao Li-jia, Li Qi-meng, Li Jin-ling, Xu Qiong. Expression pattern of ten-eleven translocation family during differentiation of human dental pulp cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2261-2266.

Figures/Tables 3

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