Oxidative stress response in diabetic nephropathy rats following injection of embryonic stem cells via the tail vein

doi:10.3969/j.issn.2095-4344.2015.14.012

Abstract

Abstract:

BACKGROUND: Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body.
OBJECTIVE: To investigate the effect of embryonic stem cells on oxidative stress response of rats with diabetic nephropathy．
METHODS: Primarily cultured rat embryonic stem cells were observed for cell morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cells via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneally injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group; contents of malondialdehyde and protein carbonyl were detected in the kidney; the expression level of superoxide dismutase was detected by western blot assay.
RESULTS AND CONCLUSION: The embryonic stem cells were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatically in the model group and experimental group (P < 0.05); compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P < 0.05). Taken together, embryonic stem cells can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Embryonic Stem Cells, Diabetic Nephropathies, Oxidative Stress

CLC Number:

R394.2

Fan Zhi-gang, Fan Hong-liang. Oxidative stress response in diabetic nephropathy rats following injection of embryonic stem cells via the tail vein[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2199-2204.

Figures/Tables 2

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