Nerve growth factor-modified adipose derived stem cells for repair of spinal cord injury

doi:10.3969/j.issn.2095-4344.2015.14.016

Abstract

Abstract:

BACKGROUND: Gene-modified stem cells can increase the secretion of peptides and full-length proteins to protect spinal cord injury and promote recovery of neuronal function, which thus become a research hotspot in recent years.

OBJECTIVE: To investigate the effect of nerve growth factor-modified adipose derived stem cells in repairing spinal cord injury in rats.

METHODS: Adipose derived stem cells were primarily cultured by adherent culture method and cell surface markers were detected by immunofluorescence method, while spinal cord injury models were set by modified Allen method. Nerve growth factor plasmid was transfected into adipose derived stem cells with Lipofectamine2000 and the expression of nerve growth factor was detected by real-time PCR and western blot. The modified adipose derived stem cells intervened by nerve growth factor were injected into the injured part of spinal cord injury rat models. Basso-Beattie-Bresnahan score was used to evaluate the repairing effect. Models rats were sacrificed at 3 weeks after cell transplantation. Real-time PCR and western blot were used to testify nerve growth factor expression in the injured spinal segment after cell transplantation.

RESULTS AND CONCLUSION: Adipose derived stem cells were successfully cultured primarily, and positive for CD29 and CD44; the mRNA and protein expression of nerve growth factor was elevated after plasmid transfection. The Basso-Beattie-Bresnahan score was elevated after transplantation of adipose derived stem cells intervened by nerve growth factor modification compared to control group; and the expression of nerve growth factor in the injured segment of the spinal cord was up-regulated detected by real-time PCR and western blot. These findings indicate that the nerve growth factor-modified adipose derived stem cells have repairing effects on spinal cord injury in rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Stem Cells, Adipose Tissue, Nerve Growth Factor, Spinal Cord Injuries

CLC Number:

R394.2

Wang Yong, Zhao Wei, Feng Jian-zhou, Chen Xiao-chun. Nerve growth factor-modified adipose derived stem cells for repair of spinal cord injury [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2224-2229.

Figures/Tables 4

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