Ultrastructure and phagocytotic function of human placental mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.14.017

Abstract

Abstract:

BACKGROUND: Placental mesenchymal stem cells with rich sources are similar to bone marrow mesenchymal stem cells in terms of morphology, surface markers and differentiation potential, which are one of ideal mesenchymal stem cells in human body. However, there are few studies addressing the ultrastructure and phagocytotic function of human placental mesenchymal stem cells and its physiological role in the the placenta has been little explored.

OBJECTIVE: To investigate the ultrastrcture and phagocytotic function of placental mesenchymal stem cells.

METHODS: Placental mesenchymal stem cells obtained from five placentae of normal pregnancy were cultured in vitro and observed for ultrastructure under transmission electron microscope. The fluorescent beads were added in the supernatant for 3 hours, and then the phagocytosis of placental mesenchymal stem cells was evaluated by flow cytometry.

RESULTS AND CONCLUSION: Under the transmission electron microscope, placental mesenchymal stem cells had large nuclei with prominent nucleoli. In the cytoplasm, a plenty of rough endoplasmic reticula was seen, dilated or stacked. The cytoplasm was also rich in Golgi apparatus and lysosomes. The cell surfaces were covered by microvilli. The intercellular junctions could be seen occasionally. A part of cells from these five samples could phagocytose fluorescence beads, which ranged from 49.6% to 18.4%. The ultrastructural characteristics of placental mesenchymal stem cells suggested these cells were active to synthesize and secrete proteins and had phagocytotic function, indicating placental mesenchymal stem cells may play a role in keeping the balance of micro-environments and clean the foreign substances in the placenta.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Placenta, Mesenchymal Stem Cells, Cellular Structures, Microscopy, Electron, Transmission, Phagocytosis

CLC Number:

R394.2

Sha Wen-qiong, She Rui-lian, Wang Zi-neng, Ke Ru. Ultrastructure and phagocytotic function of human placental mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2230-2235.

Figures/Tables 3

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