CD4+T and Treg immunomodulatory functions after culture with the supernatant of human placenta mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.0696

Abstract

Abstract:

BACKGROUND: Treg cells belong to inhibitory CD4⁺T cells. Human placenta mesenchymal stem cells (hPAMSCs) have T cell immunomodulatory function.
OBJECTIVE: To investigate the CD4⁺T and Treg cells immunomodulatory ability after culture with the supernatant of hPAMSCs.
METHODS: hPAMSCs were isolated and cultured, and the stable cell lines were then established. Passage 4 hPAMSCs and its supernatant were collected, and the expression of cell surface markers CD90, CD73, CD45 and HLA-DR were detected by flow cytometry. Cell activation cocktail stimulated peripheral blood mononuclear cells from healthy donor were co-cultured with hPAMSCs supernatant for 6 hours. The percentage of CD4⁺T cells and Treg cells was detected by flow cytometry. ELISA was used to test the level of transforming growth factor β in the cell supernatant.
RESULTS AND CONCLUSION: Successfully established primary hPAMSCs cell lines had stable passage. Supernatant of hPAMSCs significantly inhibited the percentage of CD4⁺T cells (P < 0.05), while significantly enhanced the percentage of Treg cells (P < 0.05). Meanwhile, the level of transforming growth factor β was significantly up-regulated (P < 0.01). In conclusion, the supernatant of hPAMSCs has the immunomodulatory function via inducing the conversion from CD4⁺T to Treg cells, which is expected to be a new therapeutic treatment for immune disorders.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Placenta, Mesenchymal Stem Cells, CD4-Positive T-Lymphocytes, T-Lymphocytes, Regulatory, Transforming Growth Factor beta, Tissue Engineering

CLC Number:

R459.9

Li Weiwei, Li Xiaofeng, Hou Ping, Li Jianping. CD4⁺T and Treg immunomodulatory functions after culture with the supernatant of human placenta mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(1): 85-89.

Figures/Tables 1

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