Different sources of mesenchymal stem cells in the treatment of liver fibrosis in rats

doi:10.3969/j.issn.2095-4344.2014.06.017

Abstract

Abstract:

BACKGROUND: In recent years, studies have shown that mesenchymal stem cells can secrete various growth factors, and has certain application prospect in the treatment of liver fibrosis.

OBJECTIVE: To explore the different sources of mesenchymal stem cells in the treatment of liver fibrosis in rats.

METHODS: Fifty Sprague-Dawley rats were given intraperitoneal injection of carbon tetrachloride to construct the model of liver fibrosis. Another 10 normal Sprague-Dawley rats were used as normal controls. Model rats were randomly divided into five groups, 10 rats in each group, including model control group, normal saline group, bone marrow mesenchymal stem cell group, umbilical cord mesenchymal stem cell group, and umbilical cord blood mesenchymal stem cell group. After 8 weeks of modeling, different sources of mesenchymal stem cells at a density of 2×10⁶ were injected via tail vein into model rats. After 12 weeks, the rats were killed, and serum alanine aminotransferase, aspartate aminotransferase and albumin levels were detected as well as the expression of type I collagen and glial fibrillary acidic protein in liver tissue was detected by fluorescence quantitative PCR method.

RESULTS AND CONCLUSION: We have successfully established the SD rat model of hepatic fibrosis. Bone marrow mesenchymal stem cells aggravated hepatic fibrosis, umbilical cord blood and umbilical cord mesenchymal stem cells could reduce the level of hepatic fibrosis in rats. Umbilical cord mesenchymal stem cells had the most obvious effect that significantly reduced expressions of alanine aminotransferase, aspartate aminotransferase and type I collagen and glial fibrillary acidic protein. The results showed different sources of mesenchymal stem cells have different effects on rat fibrosis. Bone marrow mesenchymal stem cells aggravate hepatic fibrosis, and umbilical cord and umbilical cord blood stem cells can alleviate hepatic fibrosis in rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cell transplantation, bone marrow, umbilical cord, fetal blood, liver cirrhosis, rats, Sprague-Dawley

CLC Number:

R394.2

Qu Xin, Wang Xin-chao, Han Lu, Zhang Hai-chao. Different sources of mesenchymal stem cells in the treatment of liver fibrosis in rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2014.06.017.

Figures/Tables 2

2.1 实验动物数量分析 60只SD大鼠均进入结果分析。 2.2 间充质干细胞形态学观察原代培养第4天，脐带间充质干细胞多呈成纤维样细胞形态，第3代脐带间充质干细胞形态均一，呈鱼群样排列生长(图1A，B)。经传代培养到第2代时，骨髓间充质干细胞呈漩涡状贴壁生长(图1C)，第4代时细胞形态较单一，状态饱满，呈梭形生长(图1D)。原代贴壁脐血间充质干细胞，呈漩涡状排列，第3代贴壁脐血间充质干细胞形态未见明显变化(图1E，F)。 2.3 间充质干细胞的表型鉴定对第3代脐带间充质干细胞进行流式细胞仪检测表明：细胞表达CD13(98%)、CD29(96%)、CD44(97%)间充质干细胞抗原标志，几乎不表达造血细胞表型CD45(0.7%)、内皮细胞的特征性表型CD31(1.9%)。对第3代骨髓间充质干细胞进行流式细胞仪检测表明：细胞表达CD73(97%)、CD105(93%)间充质干细胞抗原标志，几乎不表达造血细胞表型CD45 (1.7%)，CD34(2.0%)。对第3代脐血间充质干细胞进行流式细胞仪检测表明：细胞表达CD29、CD73(SH3)，几乎不表达CD31(内皮细胞特异性抗原标记)，CD34(造血干细胞阳性标记)。 2.4 不同组织来源的间质干细胞对SD大鼠肝纤维化功能的影响结果显示模型对照组大鼠的血清谷丙转氨酶、谷草转氨酶的值明显高于正常大鼠，白蛋白的水平明显低于正常大鼠，提示模型组大鼠肝脏功能出现损伤，骨髓间充质干细胞组大鼠血清谷丙转氨酶、谷草转氨酶活性明显高于模型对照组大鼠，白蛋白明显低于模型对照组大鼠，提示骨髓间质干细胞能进一步加重肝脏功能的损伤，脐血间充质干细胞组和脐带间充质干细胞组大鼠的血清谷丙转氨酶、谷草转氨酶的值较模型对照组明显下降，白蛋白的水平较模型对照组明显升高，脐带间充质干细胞组大鼠的血清谷丙转氨酶、谷草转氨酶值较脐血间充质干细胞组大鼠降低明显，白蛋白的水平较脐血间充质干细胞组升高明显(P < 0.05，表1)。"

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