Platelet-rich plasma plus human umbilical cord mesenchymal stem cells for cartilage repair

doi:10.3969/j.issn.2095-4344.2014.41.013

Abstract

Abstract:

BACKGROUND: Chondrocytes co-cultured with bone marrow stromal stem cells on the scaffold of platelet-rich plasma are found to proliferate, and besides proliferative growth, bone marrow stromal cells exhibit a tendency of differentiating into chondrocytes.

OBJECTIVE: To study the effect of platelet-rich plasma and human umbilical cord mesenchymal stem cells (hUCMSCs) on cartilage repair.

METHODS: Forty healthy New Zealand white rabbits were selected to establish models of cartilage defects, and then randomly divided into normal saline group, platelet-rich plasma group, hUCMSCs group and combination group. Platelet-rich plasma was prepared by using double centrifugations to prepare passage 3 hUCMSCs. After modeling, intra-articular injection of normal saline (0.5 mL), 12.5% platelet-rich plasma (0.5 mL), 1×10⁷hUCMSCs (0.5 mL), 12.5% platelet-rich plasma+1×10⁷ hUCMSCs (totally 0.5 mL) was done in corresponding groups, respectively. After 12 weeks of modeling, the injured cartilage was grossly observed, and hematoxylin-eosin staining was used to observe cartilage repair under light microscope; according to the O'Driscoll histologic standard, histological examination was performed.

RESULTS AND CONCLUSION: The repair effect in the normal saline group was significantly better that in the platelet-rich plasma group, hUCMSCs group, combination group (P < 0.05), while the platelet-rich plasma group and combination group also exhibit better outcomes than the hUCMSCs group (P < 0.05). These findings indicate that both platelet-rich plasma and hUCMSCs can promote cartilage repair; moreover, platelet-rich plasma with or without hUCMSCs is superior to hUCMSCs alone in the cartilage repair.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: platelet-rich plasma, mesenchymal stem cells, chondrocytes

CLC Number:

R394.2

Xu Jing1, Wang Li-ming, Zhou Li-dong, Wu Mei, Cui Hui, Zhao Jing, Zeng Du-juan, Zhang Zhong-wen, Liu Ai-bing. Platelet-rich plasma plus human umbilical cord mesenchymal stem cells for cartilage repair[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6633-6638.

Figures/Tables 3

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