Preparation and evaluation of chitosan/ellagic acid/erythrocyte membrane lipid hemostatic composite sponge

doi:10.3969/j.issn.2095-4344.2013.12.012

Abstract

Abstract:

BACKGROUND: Some previous studies have indicated that the hemostatic effect of chitosan is limited when dealing with severe injuries. Therefore, the procoagulant activity of chitosan-based hemostatic agents needs to be enhanced.
OBJECTIVE: To prepare a novel chitosan/ellagic acid/erythrocyte membrane lipid composite sponge and to evaluate its procoagulant activity and cytotoxicity.
METHODS: Chitosan sponge and chitosan acetate sponge were prepared by freeze-drying method. Then chitosan/ellagic acid/erythrocyte membrane lipid composite sponge was prepared by electrostatic adsorption method. Procoagulant activity of the chitosan, chitosan acetate, and chitosan/ellagic acid/erythrocyte membrane lipid sponges was evaluated by the plasma recalcification time method. Hemostatic effect of these sponges was evaluated in the Sprague Dawley rat liver bleeding model, and the cytotoxicity to L929 cell line was evaluated.
RESULTS AND CONCLUSION: The plasma recalcification time, bleeding time and blood loss of the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge group were significantly lower than those of the chitosan sponge and chitosan acetate sponge groups (P < 0.01). Cell culture experiment showed that the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge did not have cytotoxicity. The results indicate that the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge has the enhanced procoagulant activity and non-cytotoxicity.

Key words: biomaterials, chitosan, ellagic acid, erythrocyte membrane lipid, sponge, hemostasis, the National Natural Science Foundation of China

CLC Number:

R318

He Qing, Ao Qiang, Wang Zhen, Liu Wei-qiang, Gong Kai, Gong Yan-dao, Zhang Xiu-fang. Preparation and evaluation of chitosan/ellagic acid/erythrocyte membrane lipid hemostatic composite sponge[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(12): 2172-2176.

Figures/Tables 3

References

[1] He Q,Ao Q,Gong K,et al.Preparation and characterization of chitosan-heparin composite matrices for blood contacting tissue engineering. Biomed Mater. 2010;5(5):055001.

[2] Ao Q,Wang A,Cao W,et al. Manufacture of multimicrotubule chitosan nerve conduits with novel molds and characterization in vitro.J Biomed Mater Res A. 2006;77(1):11-18.

[3] Malette WG,Quigley HJ,Gaines RD,et al. Chitosan: a new hemostatic. Ann Thorac Surg. 1983;36(1):55-58.

[4] Yang J,Tian F,Wang Z,et al.Effect of chitosan molecular weight and deacetylation degree on hemostasis. J Biomed Mater Res B. 2008;84(1):131-137.

[5] Peng T. Biomaterials for hemorrhage control. Trends Biomater Artif Organs. 2010;24(1):27-68.

[6] Englehart MS,Cho SD,Tieu BH,et al.A novel highly porous silica and chitosan-based hemostatic dressing is superior to HemCon and gauze sponges. J Trauma. 2008;65(4):884-890.

[7] Vekiari SA,Gordon MH,Garcia-Macias P,et al. Extraction and determination of ellagic acid content in chestnut bark and fruit.Food Chem. 2008;110(4):1007-1011.

[8] Damas J,Remacle-Volon G,Adam A.Some cardiovascular and hematological changes induced in the rat by activation of Hageman factor with ellagic acid. Adv Exp Med Biol.1989; 247A:461-465.

[9] Zhou J,Zheng Y,Shi J,et al. Daunorubicin induces procoagulant response through phosphatidylserine exposure in red blood cells.Thromb Res. 2010;125(2):178-183.

[10] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30. 中华人民共和国科学技术部.关于善待实验动物的指导性意见. 2006-09-30.

[11] Mawatari S,Yunoki K,Sugiyama M,et al. Simultaneous preparation of purified plasmalogens and sphingomyelin in human erythrocytes with phospholipase A1 from Aspergillus orizae. Biosci Biotechnol Biochem. 2009;73(12):2621-2625.

[12] Liu C,Yang S,Liu W,et al.Preparation and characterization of medium-chain fatty acid liposomes by lyophilization. J Liposome Res. 2010;20(3):183-190.

[13] Tuzlakoglu K,Alves CM,Mano JF,et al.Production and characterization of chitosan fibers and 3-D fiber mesh scaffolds for tissue engineering applications.Macromol Biosci.2004;4(8):811-819.

[14] He Q,Gong K,Ao Q,et al.Positive charge of chitosan retards blood coagulation on chitosan films.J Biomater Appl.2011 [Epub ahead of print].

[15] Ho CH. The hemostatic effect of packed red cell transfusion in patients with anemia. Transfusion.1998;38(11-12):1011-1014.