Antibacterial properties of titanium oxide coating with silver ions

doi:10.3969/j.issn.2095-4344.0988

Abstract

Abstract:

BACKGROUND: Silver nanoparticle is a potential coating material with resistance ability to infection. Using the ion implantation method, we can make silver nanoparticles tightly connect with the base material, and meanwhile effectively control the content of silver nanoparticles. Titanium oxide coating has good biocompatibility, which is a good choice for the preparation of internal fixation materials. But the in vivo antibacterial activity of titanium oxide coating with silver ions is rarely reported, and the relationship between the content of silver nanoparticles and the antibacterial activity is still unknown.

OBJECTIVE: To investigate the antibacterial of silver plasma immersion ion implantation implants in vivo.

METHODS: Forty New Zealand white rabbits were randomized into four groups, and animal models of bone marrow infection of the distal femur were made in all the rabbits. Model rabbits were subjected to the implantation of titanium rob in control group, implantation of titanium oxide-coated implants in experimental group, implantation of implants covered with titanium oxide coating with low concentration of silver ions in low concentration group, and implantation of implants covered with titanium oxide coating with high concentration of silver ions in high concentration group. We assessed the antibacterial activities of the implants by blood test, radiographic observation and bacteriological methods thereafter.

RESULTS AND CONCLUSION: (1) After modeling, the body temperature in all the animals increased to different extents, and the body weight decreased, and gradually recovered at 14-21 days after modeling. (2) At 7, 14, 21, 28 days after modeling, white blood cell count and C-reactive protein level in the titanium rod group and titanium oxide group were significantly higher than those in the low and high concentration groups (P < 0.05). At 7 days after modeling, the percentage of neutrophils in the titanium oxide group was significantly higher than that in the low and high concentration groups (P < 0.05). At 14, 21, 28 days after modeling, the percentage of neutrophils in the titanium rod group and the titanium oxide group was significantly higher than that in the low and high concentration groups (P < 0.01). (3) At 14 and 28 days after modeling, the Norden scores in the low and high concentration groups were significantly lower than those in the titanium rod group and the titanium oxide group (P < 0.05). (4) The number of bacterial colonies in the titanium rod group and titanium oxide groups was significantly higher than that in the low and high concentration groups (P < 0.01), while the bacterial colony count in the low concentration group was significantly higher than that in the high concentration group (P < 0.01). Findings from the present study reveal that the titanium oxide-coated implant with silver ions is an antibacterial implant, and its antibacterial property is closely related to the concentration of silver ions, that is, a higher concentration of silver ions indicates stronger antibacterial property. Therefore, the use of titanium oxide coating with silver ions may provide an effective solution for implant-related infections.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Silver, Titanium, Implants, Experimental, Infection, Tissue Engineering

CLC Number:

R318

Li Gen, Song Hui, Wang Yi-han, Zhao Wen-bo, Dai Ying, Guo Yinghuimin, Dong Yu-qi, Zhang Chao. Antibacterial properties of titanium oxide coating with silver ions[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(30): 4870-4875.

Figures/Tables 10

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