Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (20): 3182-3189.doi: 10.12307/2024.354
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Gao Yuguang, Zhong Jie, Huang Deqing, Ma Yujuan, Liao Yuxiong, Liu Qiqi
Received:
2023-05-10
Accepted:
2023-06-07
Online:
2024-07-18
Published:
2023-09-09
Contact:
Zhong Jie, Master, Associate chief physician, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
About author:
Gao Yuguang, Master, Attending physician, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Gao Yuguang, Zhong Jie, Huang Deqing, Ma Yujuan, Liao Yuxiong, Liu Qiqi. Sequencing, verification and functional analysis of differentially expressed genes in brain tissue of a rat model with acute intracerebral hemorrhage[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(20): 3182-3189.
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2.3 差异基因筛选结果 见图2。脑出血组大鼠的脑组织可见明显出血灶(图2A)。PCA图(图2B)和UMAP图(图2D)表示脑出血组和假手术组的样本分开,两组之间差异明显,后续的差异基因分析有意义。以log2FC的绝对值> 1和校正P < 0.05为标准分析,经过分析,总共筛选出81个差异基因,其中包含8个表达上调基因和73个表达下调基因,将该数据集差异基因进行可视化(图2C,E);同时根据logFC数值大小筛选出排名最靠前的20个的差异基因:CXCL8、CCL20、SERPINE1、METTL14、TFPI2、CXCR4、HCN1、GDA、ANO3、KCNQ5、WIF1、KCNJ4、ERICH3、RAB27B、SCN3B、ENC1、SLITRK4、VSTM2A、HNRNPC、CACNA1E,见表3。"
2.8.1 GO分析 生物过程(biological processes,BP):DEGs主要富集于白细胞趋化性(leukocyte chemotaxis)、趋化因子介导的信号通路(chemokine-mediated signaling pathway)、血管创伤的调节(regulation of vascular wound);细胞组成(cellular components,CC):DEGs主要富集于阳离子通道复合物(cation channel complex)、离子通道复合物(ion channel complex)、跨膜转运蛋白复合物(transmembrane transporter complex);分子功能(molecular functions,MF):DEGs主要富集于门控通道活动(gated channel activity)、离子通道的活动(ion channel activity)、通道的活性(channel activity)。 2.8.2 KEGG通路富集分析 DEGs集中于TNF信号通路(TNF signaling pathway),谷氨酸能突触(Glutamatergic synapse)、GABA能突触(GABAergic synapse)。"
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