Repair effects of two kinds of bone repair materials on rabbit femoral defects compared using synchrotron-radiation-based micro-computed tomography

doi:10.12307/2023.460

Abstract

Abstract: BACKGROUND: Bio-oss bone powder and Aobang repair materials are commonly used bone repair materials, but there are few reports on their systematic evaluation of the repair effect of rabbit femoral defect.
OBJECTIVE: To systematically evaluate the repair effects of Bio-oss bone powder and Aobang repair materials on femoral defects by using synchrotron-radiation-based micro-computed tomography and histopathological staining.
METHODS: Thirty-two rabbits were randomly divided into the control group (n=12) and the experimental group (n=20) by the random number table method. Two holes with a size of 4 mm (the diameter of the cavity) × 6 mm (hole depth) of penetrating periosteal bone defect were established on the lateral surface of one side of the femoral body. The control group was filled with normal saline. In the experimental group, Bio-oss bone powder was implanted in one of the bone defects, and Aobang repair material was implanted in the other bone defect. After 8 weeks, the effects of the two kinds of bone repair materials were evaluated by visual observation, imaging analysis and histopathological examination.
RESULTS AND CONCLUSION: (1) General view: 8 weeks after the operation, the wounds in the repaired area of bone injury in the experimental group healed well; no infection, suppuration or other symptoms were found, and the animal mobility was significantly better than that of the control group. (2) Imaging examination: synchrotron-radiation-based micro-computed tomography 8 weeks after the operation exhibited that after the implantation of Bio-oss bone powder, there was a large amount of new bone tissue around the bone powder, and the cavity of the bone defect was large, which was not completely filled by the new bone tissue. After the implantation of the Aobang repair material, a large amount of new bone tissue at the bone defect cavity was formed around the repair material. 3D reconstruction showed that both bone filling materials could contact with local bone wound tissue, promote the surrounding osteogenesis to differentiate into new bone tissue and blood vessel formation, and achieve bone repair effect. The new bone around the Aobang repair material was more closely connected with the surrounding bone tissue. 256 iCT scan showed that the CT value of the Aobang repair material side at 8 weeks after operation was higher than that of the Bio-oss bone powder side (P < 0.05). (3) Histopathology: At 8 weeks after operation, hematoxylin-eosin staining showed that new bone tissue could be seen around the side of the Bio-oss bone powder. Bone tissue formed by bone matrix could be seen around the Aobang repair material; the new bone tissue was complete and coherent, and some of the new bone had invaded into the Aobang repair material. (4) These results suggest that Aobang repair material can promote the formation of new bone and damage repair; synchrotron-radiation-based micro-computed tomography echoes the traditional hematoxylin-eosin staining, and it is a good method to evaluate the effect of bone powder repair.

Key words: bone repair material, Aobang repair material, Bio-oss bone powder, femoral defect, tissue engineering, synchrotron-radiation-based micro-computed tomography

CLC Number:

Sun Lianlian, Liu Yongchao, Wang Zhixing. Repair effects of two kinds of bone repair materials on rabbit femoral defects compared using synchrotron-radiation-based micro-computed tomography[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(21): 3343-3348.

Figures/Tables 6

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