Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (21): 3349-3355.doi: 10.12307/2023.170
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Huang Senli1, Sun Haigang1, Sun Wenquan2
Received:
2022-03-24
Accepted:
2022-05-13
Online:
2023-07-28
Published:
2022-11-24
Contact:
Sun Wenquan, PhD, Professor, Doctoral supervisor, School of Health Science and Engineering, Shanghai University for Science and Technology, Shanghai 200093, China
About author:
Huang Senli, Master, Ruijian Gaoke Biotechnology Co., Ltd., Beijing 102200, China
CLC Number:
Huang Senli, Sun Haigang, Sun Wenquan. Physical and chemical properties of porcine dermis matrix affected by process integration for decellularization, viral inactivation and sterilization[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(21): 3349-3355.
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A组脱细胞组织基质材料与未处理猪真皮样品相似,染色很深,说明方法A对降低α-Gal抗原没有效果。方法B制备的脱细胞组织基质材料免疫组化染色为阴性,α-Gal抗原表位去除彻底。 2.5 两组基质材料的热稳定性 图8比较了用方法A和方法B制备的组织基质的热稳定性。与未处理的猪真皮相比,A组脱细胞后材料的热稳定性曲线没有明显变化,如初始(Td)或峰值变性温度、蛋白质变性的热焓值及热图的形状都没有变化。B组脱细胞后材料初始变性温度(Td)降低3.0-4.0 ℃。然而,经伽马射线照射后,A组组织基质变得很不稳定,起始变性温度Td下降到(46.2±1.6) ℃,变性峰值温度下降到(51.2±1.8) ℃,与辐照前的脱细胞组织基质比较,热变性起始温度下降了15 ℃;B组组织基质对辐照灭菌具有较高的耐受性,起始Td大于53 ℃,与辐照前的对比,热变性起始温度仅下降了3 ℃。因此,采用不同的脱细胞制备工艺,材料经过与病毒灭活和灭菌工艺的整合影响材料的稳定性。实验采用的这两种方法制备的组织基质材料热稳定性有显著差异。"
2.6 两组基质材料的力学性能 两组组织基质材料力学性能存在差异。结果显示,伽马射线终端灭菌后,A组基质材料在拉伸至应力为5 N时的低应变回弹区间,应变值为(14.8±3.1)%,B组基质材料相对应的应变值为(24.0±5.7)%;与B组相比,A组基质材料延展性较差。A组基质材料最大载荷为(150±47) N,B组基质材料最大载荷为(210±59) N。与B组相比,A组基质材料的强度略有下降。A、B组材料的断裂伸长率分别为(69±12)%和(65±12)%,没有明显差异。A、B组材料的杨氏模量分别为(24.6±8.8) MPa和(44.8±5.4) MPa。与B组相比,A组基质材料的抵抗形变能力或刚度较差。 2.7 两组基质材料的体外酶解性能 方法A和方法B制备的组织基质材料抗蛋白酶降解的能力不同。在伽马射线终端灭菌后,A组基质材料对Ⅰ型胶原酶具有很强的抗性,处理72 h后仅有约20%的降解,B组基质材料15 h内减少到50%,见图9。不过,A组基质材料对胰蛋白酶的抗性下降,胰蛋白酶可以降解80%以上的基质材料,B组基质材料只能降解小于35%的材料,见图10。对两个酶的抗性,方法B制备的基质材料与没有经过脱细胞处理的猪真皮材料极为相似。"
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