Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (20): 3266-3272.doi: 10.12307/2023.444

Previous Articles     Next Articles

hFOB1.19 application in bone tissue engineering

Wang Yanyang, Xu Pu   

  1. Department of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Center, Haikou 570208, Hainan Province, China
  • Received:2022-05-20 Accepted:2022-07-21 Online:2023-07-18 Published:2022-11-21
  • Contact: Xu Pu, MD, Chief physician, Department of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Center, Haikou 570208, Hainan Province, China
  • About author:Wang Yanyang, Master candidate, Department of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Center, Haikou 570208, Hainan Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 82060194 (to XP); 2022 Key R&D Project of Hainan Province, No. ZDYF2022SHFZ119 (to XP)

Abstract: BACKGROUND: The biological characteristics of animal osteoblast models widely used at this stage are quite different from those of human cells. The primary human osteoblasts isolated and cultured are difficultly purified, and the culture generations are limited. Osteosarcoma cells have the risk of abnormal growth, which cannot fully meet the research requirements. In 1995, Harris transfected human fetal limb cells with simian vacuolating virus 40, and screened a group of immortalized human osteoblast cell line hFOB1.19 that differentiated into earlier osteogenic progenitor cells.
OBJECTIVE: To review the basic characteristics, culture methods and application of hFOB1.19 as a cell model of bone tissue engineering materials. 
METHODS: Relevant literature published from 1995 to May 2022 was retrieved in CNKI, WanFang, SinoMed, PubMed, Web of Science, Medicine, and Cochrane Library databases. The keywords were “hFOB1. 19, human osteoblast cell line, immortalized cells, monkey vacuolating virus 40, bone tissue engineering, bone substitute materials, biomaterials research” in Chinese and English, respectively. Finally, 73 papers were screened for review. 
RESULTS AND CONCLUSION: hFOB1.19 is highly similar to osteoblasts in vivo in terms of morphology, phenotype, karyotype, biological characteristics, and differentiation potential, which has the ability of rapid and stable growth. hFOB1.19 has been widely used in cell adhesion, proliferation, differentiation, mineralization, gene expression, and protein synthesis to test the biocompatibility and osteogenic properties of bone tissue engineering materials. hFOB1.19 can make up for the shortcomings of different animal cell sources, abnormal proliferation of osteosarcoma cells, difficult separation of primary osteoblasts, and few passages. It has stable performance and good proliferation in bone tissue engineering experiments. However, due to the special culture conditions, limitation of cell passage number and biosafety, it is not widely used in the field of bone tissue engineering materials. At present, the development of bone tissue engineering materials is fast, but there is a lack of a systematic and comprehensive research system and popularization of a unified research standard in the development process. This article summarizes the application of hFOB1.19 in different studies to provide researchers with guidance for method selection and improve relevant standards.

Key words: human fetal osteoblast cell line 1.19, human osteoblast cell line, immortalized cell, simian vacuolating virus 40, bone tissue engineering, bone substitute material, biomaterial research

CLC Number: