Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (20): 3121-3126.doi: 10.12307/2022.608
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Liu Qiqi1, Liu Min2, Yang Jian1, Yu Ke1
Received:
2021-07-06
Accepted:
2021-08-19
Online:
2022-07-18
Published:
2022-01-18
Contact:
Yu Ke, MD, Associate professor, Department of Oral Implantology, School/Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:
Liu Qiqi, Master candidate, Department of Oral Implantology, School/Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
CLC Number:
Liu Qiqi, Liu Min, Yang Jian, Yu Ke. Lipopolysaccharide stimulates the expression of interleukin-6 and nuclear factor kappa B receptor activator ligand in mouse MLO-Y4 osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(20): 3121-3126.
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由于AKT磷酸化激活PI3K/AKT信号途径,实验分析p-AKT/AKT蛋白比率以确定PI3K/AKT在脂多糖刺激白细胞介素6和RANKL表达中的作用。与对照组比较,脂多糖处理1 h后的p-AKT/AKT蛋白比率增加(P < 0.05),6 h后的p-AKT/AKT蛋白比率显著高于其他时间点(P < 0.05),然后p-AKT/AKT蛋白比率下降,各时间点间两两比较均差异均有显著性意义(P < 0.05),见图2。 2.3 PI3K抑制剂降低脂多糖诱导的骨细胞白细胞介素6和RANKL mRNA表达 与对照组比较,脂多糖处理后骨细胞的白细胞介素6和RANKL mRNA表达显著升高(P < 0.05)。与单独脂多糖处理比较,不同浓度的LY294002预处理+脂多糖处理组骨细胞的白细胞介素6和RANKL mRNA表达显著降低(P < 0.05);其中10 μmol/L LY294002预处理+脂多糖处理组与20 μmol/L LY294002预处理+脂多糖处理组骨细胞的白细胞介素6和RANKL mRNA表达比较差异无显著性意义(P > 0.05),但均低于其他浓度LY294002预处理+脂多糖处理组(P < 0.05),见图3。"
2.5 LY294002或二甲基亚砜对脂多糖诱导骨细胞RANKL表达与AKT磷酸化的影响 与对照组比较,脂多糖组骨细胞的RANKL mRNA和蛋白表达显著升高(P < 0.05),p-AKT蛋白表达升高(P < 0.05)。与脂多糖组比较,二甲基亚砜组骨细胞的RANKL mRNA和蛋白表达显著降低(P < 0.05),p-AKT蛋白表达显著降低(P < 0.05),但是并未降到对照组的水平,与对照组比较差异仍有显著性意义(P < 0.05),说明二甲基亚砜部分阻断了PI3K/AKT信号通路。与脂多糖组比较,LY294002组骨细胞的RANKL mRNA和蛋白表达显著降低(P < 0.05),p-AKT蛋白表达显著降低(P < 0.05),但是RANKL表达并未降到对照组的水平,与对照组比较差异仍有显著性意义(P < 0.05),说明10 μmol/L的LY294002可完全抑制AKT的磷酸化,但不能完全阻断脂多糖诱导的RANKL表达,见图6。 "
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