Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (24): 3790-3795.doi: 10.12307/2022.555
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Yan Nan1, Wu Yanlong2, Tang Xiaohui2, Zhang Xiaoyan2, Wang Hui2, Yang Tianze2, Zhou Maochun2, Wang Zhengdong3, Yang Xiaoxia1
Received:
2021-02-25
Accepted:
2021-04-10
Online:
2022-08-28
Published:
2022-01-22
Contact:
Yang Xiaoxia, Master, Professor, School of Medical Applied Technology, Shenyang Medical College, Shenyang 110034, Liaoning Province, China
About author:
Yan Nan, MD, Associate professor, School of Medical Applied Technology, Shenyang Medical College, Shenyang 110034, Liaoning Province, China
Supported by:
CLC Number:
Yan Nan, Wu Yanlong, Tang Xiaohui, Zhang Xiaoyan, Wang Hui, Yang Tianze, Zhou Maochun, Wang Zhengdong, Yang Xiaoxia. Bone marrow mesenchymal stem cells may alleviate brain damage caused by the microglial overactivation in the cortex around ischemic site of stroke[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(24): 3790-3795.
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2.2 实验动物数量分析 根据Zea Longa评分评估大鼠瘫痪肢体的运动功能。模型组和移植组大鼠苏醒后对侧肢体瘫痪,无法站立或不稳定地向前移动,抬起尾巴时身体转向一侧。假手术组大鼠均为0级,其余两组8只大鼠为2级,12只大鼠为3级,均进入结果分析。 2.3 各组大鼠BBB评分 术后,模型组和移植组大鼠对侧肢体立即瘫痪,BBB评分为0。术后第1周末,模型组和移植组大鼠关节运动次数、运动负荷、伸展范围以及前后肢的协调程度都有一定程度的改善,BBB评分也逐渐升高,移植组与模型组之间的BBB评分无显著差异(P > 0.05),但是移植组与模型组的BBB评分显著低于假手术组(P < 0.001);术后第2周末,移植组BBB评分明显高于模型组(P < 0.01),移植组和模型组的BBB评分仍低于假手术组(P < 0.001),见图2。"
[1] KERR N, DIETRICH DW, BRAMLETT HM, et al. Sexually dimorphic microglia and ischemic stroke. CNS Neurosci Ther. 2019;25(12):1308-1317. [2] ZHANG D, LU Z, MAN J, et al. Wnt-3a alleviates neuroinflammation after ischemic stroke by modulating the responses of microglia/macrophages and astrocytes. Int Immunopharmacol. 2019;75:105760. [3] YAN RY, WANG SJ, YAO GT, et al. The protective effect and its mechanism of 3-n-butylphthalide pretreatment on cerebral ischemia reperfusion injury in rats. Eur Rev Med Pharmacol Sci. 2017;21(22):5275-5282. [4] GOMEZ-NICOLA D, PERRY VH. Microglial dynamics and role in the healthy and diseased brain: a paradigm of functional plasticity. Neuroscientist. 2015; 21(2):169-184. [5] DU L, ZHANG Y, CHEN Y, et al. Role of Microglia in Neurological Disorders and Their Potentials as a Therapeutic Target. Mol Neurobiol. 2017;54(10): 7567-7584. [6] YEH CF, CHUANG TY, HUNG YW, et al. Soluble epoxide hydrolase inhibition enhances anti-inflammatory and antioxidative processes, modulates microglia polarization, and promotes recovery after ischemic stroke. Neuropsychiatr Dis Treat. 2019;15:2927-2941. [7] ABDALLAH BM, HAACK-SØRENSEN M, BURNS JS, et al. Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite [corrected] extensive proliferation. Biochem Biophys Res Commun. 2005; 326(3):527-538. [8] CHAO YH, PENG CT, HARN HJ, et al. Poor potential of proliferation and differentiation in bone marrow mesenchymal stem cells derived from children with severe aplastic anemia. Ann Hematol. 2010;89(7):715-723. [9] SUN LY, HSIEH DK, SYU WS, et al. Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential. Cell Prolif. 2010;43(5):445-456. [10] XIE DM, LI YL, LI J, et al. CD51 distinguishes a subpopulation of bone marrow mesenchymal stem cells with distinct migratory potential: a novel cell-based strategy to treat acute myocardial infarction in mice. Stem Cell Res Ther. 2019;10(1):331. [11] WEISS JN, LEVY S, BENES SC. Stem Cell Ophthalmology Treatment Study (SCOTS): bone marrow-derived stem cells in the treatment of Leber’s hereditary optic neuropathy. Neural Regen Res. 2016;11(10):1685-1694. [12] WANG X, ZHAI W, ZHU J, et al. Treatment of the bone marrow stromal stem cell supernatant by nasal administration-a new approach to EAE therapy. Stem Cell Res Ther. 2019;10(1):325. [13] NAKANO M, NAGAISHI K, KONARI N, et al. Bone marrow-derived mesenchymal stem cells improve diabetes-induced cognitive impairment by exosome transfer into damaged neurons and astrocytes. Sci Rep. 2016; 6:24805. [14] HAO F, LI A, YU H, et al. Enhanced Neuroprotective Effects of Combination Therapy with Bone Marrow-Derived Mesenchymal Stem Cells and Ginkgo biloba Extract (EGb761) in a Rat Model of Experimental Autoimmune Encephalomyelitis. Neuroimmunomodulation. 2016;23(1):41-57. [15] LI J, LIU Z, WANG L, et al. Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors. Biosci Rep. 2019;39(10):BSR20190749. [16] NASOUTI R, KHAKSARI M, MIRZAEE M, et al. Trehalose protects against spinal cord injury through regulating heat shock proteins 27 and 70 and caspase-3 genes expression. J Basic Clin Physiol Pharmacol. 2019;31(1):1-8. [17] QIN C, ZHOU LQ, MA XT, et al. Dual Functions of Microglia in Ischemic Stroke. Neurosci Bull. 2019;35(5):921-933. [18] ELDAHSHAN W, FAGAN SC, ERGUL A. Inflammation within the neurovascular unit: Focus on microglia for stroke injury and recovery. Pharmacol Res. 2019; 147:104349. [19] HEINDL S, GESIERICH B, BENAKIS C, et al. Automated Morphological Analysis of Microglia After Stroke. Front Cell Neurosci. 2018;12:106. [20] SANDVIG I, AUGESTAD IL, HÅBERG AK, et al. Neuroplasticity in stroke recovery. The role of microglia in engaging and modifying synapses and networks. Eur J Neurosci. 2018;47(12):1414-1428. [21] XIONG XY, LIU L, YANG QW. Functions and mechanisms of microglia/macrophages in neuroinflammation and neurogenesis after stroke. Prog Neurobiol. 2016;142:23-44. [22] CHEN E, LIU G, ZHOU X, et al. Concentration-dependent, dual roles of IL-10 in the osteogenesis of human BMSCs via P38/MAPK and NF-κB signaling pathways. FASEB J. 2018;32(9):4917-4929. [23] PAN J, JIN JL, GE HM, et al. Malibatol A regulates microglia M1/M2 polarization in experimental stroke in a PPARγ-dependent manner. J Neuroinflammation. 2015;12:51. [24] LI D, WANG C, YAO Y, et al. mTORC1 pathway disruption ameliorates brain inflammation following stroke via a shift in microglia phenotype from M1 type to M2 type. FASEB J. 2016;30(10):3388-3399. [25] WON S, LEE JK, STEIN DG. Recombinant tissue plasminogen activator promotes, and progesterone attenuates, microglia/macrophage M1 polarization and recruitment of microglia after MCAO stroke in rats. Brain Behav Immun. 2015;49:267-279. [26] COLLMANN FM, PIJNENBURG R, HAMZEI-TAJ S, et al. Individual in vivo Profiles of Microglia Polarization After Stroke, Represented by the Genes iNOS and Ym1. Front Immunol. 2019;10:1236. [27] TIAN X, LIU H, XIANG F, et al. β-Caryophyllene protects against ischemic stroke by promoting polarization of microglia toward M2 phenotype via the TLR4 pathway. Life Sci. 2019;237:116915. [28] WANG J, XING H, WAN L, et al. Treatment targets for M2 microglia polarization in ischemic stroke. Biomed Pharmacother. 2018;105:518-525. [29] CHEN AQ, FANG Z, CHEN XL, et al. Microglia-derived TNF-α mediates endothelial necroptosis aggravating blood brain-barrier disruption after ischemic stroke. Cell Death Dis. 2019;10(7):487. [30] LIU Z, RAN Y, HUANG S, et al. Curcumin Protects against Ischemic Stroke by Titrating Microglia/Macrophage Polarization. Front Aging Neurosci. 2017; 9:233. [31] FONFRIA E, MATTEI C, HILL K, et al. TRPM2 is elevated in the tMCAO stroke model, transcriptionally regulated, and functionally expressed in C13 microglia. J Recept Signal Transduct Res. 2006;26(3):179-198. [32] ZHOU W, LIU Q, XU B. Improvement of bone defect healing in rats via mesenchymal stem cell supernatant. Exp Ther Med. 2018;15(2):1500-1504. [33] LIN J, WANG C, WU Z. Preliminary study on effects of human brain-derived neurotrophic factor gene-modified bone marrow mesenchymal stem cells by intravenous transplantation on structure and function of rat injured spinal cord. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010;24(8):982-987. [34] WANG W, HUANG X, LI J, et al. Methane Suppresses Microglial Activation Related to Oxidative, Inflammatory, and Apoptotic Injury during Spinal Cord Injury in Rats. Oxid Med Cell Longev. 2017;2017:2190897. [35] HE Y, MA X, LI D, et al. Thiamet G mediates neuroprotection in experimental stroke by modulating microglia/macrophage polarization and inhibiting NF-κB p65 signaling. J Cereb Blood Flow Metab. 2017;37(8):2938-2951. [36] 朱婷,孙桂波,孟祥宝,等.小胶质细胞/巨噬细胞的极化及在脑卒中修复中的作用[J].中国药理学通报,2019,35(8):1046-1050. [37] VON LEDEN RE, KHAYRULLINA G, MORITZ KE, et al. Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury. J Neuroinflammation. 2017;14(1):161. [38] 樊文香.缺血性脑卒中的机制研究进展[J].中国药科大学学报,2018, 49(6):751-759. |
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