中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (51): 8243-8247.doi: 10.3969/j.issn.2095-4344.2014.51.009

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

成骨细胞和成纤维细胞黏附及增殖：不同宽度表面均一平行沟槽的影响

毛菲菲1, 2，陈书慧3，聂飞龙4，陆 慧1, 2，陈 兢3，魏世成1, 2   

  1. 1北京大学口腔医学院•口腔医院，交叉学科实验室，口腔颌面外科，北京市 100081；北京大学，2前沿交叉学科研究院生物医用材料与组织工程研究中心，3微电子学研究所，4工学院，北京市 100871
  • 出版日期:2014-12-10 发布日期:2014-12-10
  • 通讯作者: 魏世成，博士，教授，北京大学口腔医学院?口腔医院，交叉学科实验室，口腔颌面外科，北京市 100081；北京大学前沿交叉学科研究院生物医用材料与组织工程研究中心，北京市 100871
  • 作者简介:毛菲菲，女，1984年生，河南省郑州市人，汉族，北京大学口腔医学院在读硕士，主要从事种植体表面微结构的研究。
  • 基金资助:

    国家重点基础研究发展计划(973)项目，科技部重大科学计划项目纳米计划专项课题纳米材料在牙组织再生与修复中的机理研究(2007CB936103)

Attachment and proliferation of fibroblasts and osteoblasts: effects of uniform micro-groove patterns with different widths

Mao Fei-fei1, 2, Chen Shu-hui3, Nie Fei-long4, Lu Hui1, 2, Chen Jing3, Wei Shi-cheng1, 2   

  1. 1Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing 100081, China; 2Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; 3Institute of Microelectronics, Peking University, Beijing 100871, China; 4College of Engineering, Peking University, Beijing 100871, China
  • Online:2014-12-10 Published:2014-12-10
  • Contact: Wei Shi-cheng, Doctor, Professor, Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
  • About author:Mao Fei-fei, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
  • Supported by:

    the National Key Basic Research Development Project (973 Program) of China, No. 2007CB936103

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摘要：

背景：生物相容性材料表面的微结构可影响真核细胞的黏附和增殖。

 

目的：观察纯钛表面的微结构对成骨细胞和成纤维细胞黏附及增殖的影响，探索优化种植体体部及穿龈部位的材料形貌特征。

 

方法：在光滑的钛片表面设计并制备深度为2 µm，宽度分别为100，50，20，10，5 µm的5种均一平行微米沟槽，以光滑钛片作为对照，测定样品的粗糙度和亲水接触角。在体外对不同形貌的钛片分别进行成骨细胞MG63和成纤维细胞L929复合培养。

 

结果与结论：微米沟槽钛片的亲水性和粗糙度较光滑钛片均有所增加。不同尺度的沟槽对不同细胞黏附和增殖的影响不同，均一平行沟槽结构普遍促进成纤维细胞的黏附和成骨细胞的增殖(P < 0.05)；而当沟槽的尺寸接近细胞的形态和大小时对细胞行为有负面影响。

 


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 成纤维细胞, 微结构, 钛片, 深反应离子刻蚀, 接触角, 表面粗糙度, 成骨细胞, 生物相容性

Abstract:

BACKGROUND: The adhesion and proliferation of eukaryotic cells is affected significantly by the microstructure of biocompatible materials.

 
OBJECTIVE: To investigate the effects of microstructure of titanium surfaces on adhesion and proliferation of osteoblasts and fibroblasts, and explore topography surfaces for implant.
METHODS: Titanium materials with five kinds (100, 50, 20, 10 and 5 µm) of uniform micro-groove surfaces were prepared in 2 µm depth, and smooth titanium plate served as the control group. The roughness of the different titanium sample and surface wettability were detected. The titanium samples of different morphologies were co-cultured with osteoblast MG63 and fibroblasts L929 in vitro.
RESULTS AND CONCLUSION: The surface wettability and surface roughness of micro-grooved titanium groups increased compared with the control group. Effects of different micro-groove surfaces had different effects on the attachment and proliferation of different cells. Uniform micro-groove surfaces generally promoted fibroblast adhesion and osteoblast proliferation (P < 0.05). However, the micro-groove of which scale was similar to cell size had a negative impact on cell behavior.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: biocompatible materials, surface properties, titanium, osteoblasts, fibroblasts

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