中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (34): 5503-5508.doi: 10.3969/j.issn.2095-4344.2014.34.016

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

种植骨髓间充质干细胞脑血管支架的性能评价

唐  瑛1，刘李娜1，王庆敏1，刘 畅2，杨义力2   

  1. 1解放军海军医学研究所，上海市  200433；2长征医院转化医学中心，解放军第二军医大学，上海市  200433
  • 修回日期:2014-07-14 出版日期:2014-08-20 发布日期:2014-08-20
  • 通讯作者: 唐瑛，解放军海军医学研究所，上海市 200433
  • 作者简介:唐瑛，女，1964年生，研究员，硕士生导师，主要从事转化医学研究。
  • 基金资助:

    上海市卫生局基金(12K004)

Performance evaluation of a cerebrovascular stent coated with bone marrow mesenchymal stem cells

Tang Ying1, Liu Li-na1, Wang Qing-min1, Liu Chang2, Yang Yi-li2   

  1. 1Naval Medical Research Institute, Shanghai 200433, China; 2Center for Translational Medicine, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200433, China
  • Revised:2014-07-14 Online:2014-08-20 Published:2014-08-20
  • Contact: Tang Ying, Naval Medical Research Institute, Shanghai 200433, China
  • About author:Tang Ying, Investigator, Master’s supervisor, Naval Medical Research Institute, Shanghai 200433, China
  • Supported by:

    the Grant of Shanghai Health Bureau, No. 12K004

PDF

370

被引次数

2

摘要：

背景：一般认为对支架表面进行修饰，尤其是种植细胞，可能会加速或导致其内皮化，避免支架内血栓形成而引起再狭窄的发生。

目的：寻找骨髓间充质干细胞种植血管支架的最佳条件。
方法：将1×106，1×107，1×108，1×109 L-1的第3代大鼠骨髓间充质干细胞分别接种至不锈钢血管支架制备细胞-支架复合物，接种48 h后电镜观察细胞形态变化。取SD大鼠160只，20只为正常对照组，其余140只制作缺血性脑卒中模型，造模8周后随机分为7组，分别植入不锈钢支架、高分子材料支架及不同浓度细胞-支架复合物，以不植入任何材料的为模型对照。植入后8周，蛋白免疫印迹技术检测植入支架上细胞的血管内皮生长因子表达，流式细胞仪检测各组大鼠血小板活化。

结果与结论：种植的干细胞均能在不锈钢支架上贴壁生长，当细胞浓度为1×107 L-1时，上皮样细胞完整覆盖支架，细胞及细胞器形态正常。缺血性脑卒中模型实验中，1×107 L-1细胞-支架组细胞生长良好，在血管内环境诱导下具有良好的内皮化趋势，并且可显著抑制血小板活化。表明当细胞种植浓度为1×107 L-1时制备的血管支架通畅，具有良好的生物学效应。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 不锈钢血管支架, 血管支架, 骨髓间充质干细胞, 血小板

Abstract:

BACKGROUND: Generally, the stent surface modification, especially seeding cells, may accelerate or cause stent endothelium, and cause restenosis for prevention of in-stent thrombosis.

OBJECTIVE: To develop the optimal conditions for vascular stents coated with bone marrow mesenchymal stem cells.
METHODS: Cerebrovascular stent was co-cultured with passage 3 bone marrow mesenchymal stem cells from rats at 1×106, 1×107, 1×108, and 1×109/L. Cells on the stents were examined with transmission electron microscopy after 48 hours. A total of 160 male Sprague-Dawley rats were enrolled, among which, 20 rats were as normal control group, and the remaining 140 were used for producing models of ischemic stroke that were randomly sub-divided into seven groups at 8 weeks after modeling: stainless steel stent implanted group, polymer stent group, and different concentrations of cell stent composite groups. After 8 weeks of implantation, the expression of vascular endothelial growth factor in these cells was examined by western blot assay. Rat platelet activation in different groups was determined by flow cytometry.
RESULTS AND CONCLUSION: Implanted stem cells were able to grow adherently on the stainless steel stent wall. When the planting cell concentration was 1×107 cells/L, the cells and organelles were morphologically normal and covered the stent surface well. These coated cells also expressed vascular endothelial growth factor, suggesting that they functioned as endothelial cells, and they also significantly lowered platelet activation. When co-cultured with 1×107/L bone marrow mesenchymal stem cells, the stent was covered well with endothelial-like cells and had significant lower platelet activation in vivo.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: stainless steel, stem cells, blood platelets

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