中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3442-3446.doi: 10.3969/j.issn.1673-8225.2012.19.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

氧体积分数变化与大鼠骨髓间充质干细胞的增殖及迁移*★

王立维，赵  渝，黄  旭，黄  文   

  1. 重庆医科大学附属第一医院血管外科，重庆市400016
  • 收稿日期:2011-10-28 修回日期:2011-12-29 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: 黄文，博士，副主任医师，副教授，重庆医科大学附属第一医院血管外科，重庆市 400016 dhuangwen@hotmail.com 并列通讯作者：赵渝，硕士，教授，博士生导师，重庆医科大学附属第一医院血管外科，重庆市 400016
  • 作者简介:王立维★，男，1986年生，四川省成都市人，汉族，2012年重庆医科大学毕业，硕士，主要从事干细胞研究。439915562@qq.com
  • 基金资助:

    重庆市教育委员会科学项目(KJ090320)。

Effects of hypoxia on proliferation and migration of rat bone marrow mesenchymal stem cells

Wang Li-wei, Zhao Yu, Huang Xu, Huang Wen   

  1. Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Received:2011-10-28 Revised:2011-12-29 Online:2012-05-06 Published:2012-05-06
  • Contact: Huang Wen, M.D., Associate chief physician, Associate professor, Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China dhuangwen@hotmail.com Corresponding author: Zhao Yu, Master, Professor, Doctoral supervisor, Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • About author:Wang Li-wei★, Master, Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 439915562@qq.com
  • Supported by:

    Research Program of Chongqing Education Commission, No. KJ090320*

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摘要：

背景：研究表明，细胞分化程度低的骨髓间充质干细胞移植在缺血环境的转归对疗效有很大影响。
目的：探讨氧体积分数改变对大鼠骨髓间充质干细胞增殖和归巢能力的影响。
方法：分离SD大鼠骨髓间充质干细胞，分别在低氧(体积分数1%O2)和常氧(体积分数20%O2)条件下培养0~7 d。细胞Transwell小室迁移实验分组为4组：体积分数1%O2培养1%O2迁移组、体积分数20%O2培养1%O2迁移组、体积分数1%O2培养20%O2迁移组和体积分数20%O2培养20%O2迁移组，比较不同氧体积分数变化条件下迁移细胞数。观察不同氧体积分数变化对间充质干细胞表达整合素β1和细胞间黏附分子1的影响，实验分为4组：恒定体积分数20%O2培养；恒定体积分数1% O2培养；体积分数20%O2培养后1%O2继续培养6 h；体积分数1%O2培养后20%O2继续培养6 h。
结果与结论：骨髓间充质干细胞在体积分数1%O2环境下增殖能力及迁移能力均增强，在体积分数20%O2环境下增殖能力及迁移能力减弱(P < 0.05)；整合素β1表达水平在体积分数1%O2环境下增强(P < 0.05)。提示大鼠骨髓间充质干细胞移植在低氧(体积分数1%O2)下增殖和迁移能力增强。
关键词：低氧；骨髓间充质干细胞；移植；整合素β1；迁移
doi:10.3969/j.issn.1673-8225.2012.19.005

关键词: 低氧, 骨髓间充质干细胞, 移植, 整合素&beta, 1, 迁移

Abstract:

BACKGROUND: Studies have demonstrated that turnover of transplanted bone marrow mesenchymal stem cells (BMSCs) with poor differentiation potential in ischemic environment greatly influences curative effects.
OBJECTIVE: To investigate the effects of hypoxia changes on proliferation and homing ability of rat BMSCs. 
METHODS: Sprague-Dawley rat BMSCs were isolated and then cultured under atmosphere of 1% O2 (hypoxia group) and 20% O2 (normoxia group) respectively for 0-7 days. Cells for Transwell chamber migration were classified into four groups by oxygen concentration at culture and migration: 1%O2 culture and 1%O2 migration, 20%O2 culture and 1%O2 migration, 1%O2 culture and 20%O2 migration, 20%O2 culture and 20%O2 migration. Migrated cells were counted. Protein levels of integrin β1 and intercellular adhesion molecule-1 in BMSCs cultured under different concentrations of oxygen were detected by western blot.
RESULTS AND CONCLUSION: Migration and proliferation abilities of BMSCs were enhanced under the atmosphere of 1%O2 and the abilities were weakened under the atmosphere of 20%O2 (P < 0.05). Integrin β1 expression in BMSCs was increased under the atmosphere of 1%O2 (P < 0.05). These findings suggest that 1% O2 enhances the proliferation and migration abilities of rat BMSCs after transplantation.

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