中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (18): 3329-3332.doi: 10.3969/j.issn.1673-8225.2012.18.022

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

低分子肝素和重组水蛭素对兔耳静脉淤血皮瓣成活的影响★

王春艳1，童庭辉2，韩  寅2，王太平2，廖  毅2   

  1. 泸州医学院附属医院，1麻醉科，  2整形烧伤科，四川省泸州市 646000
  • 收稿日期:2011-12-16 修回日期:2012-01-05 出版日期:2012-04-29 发布日期:2012-04-29
  • 通讯作者: 廖毅，教授、主任医师，泸州医学院附属医院整形烧伤科，四川省泸州市 646000
  • 作者简介:Wang Chun-yan★, Master, Attending physician, Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China tongth@163.com

Influence of low-molecular-weight heparin or recombinant hirudin on the survival of ear venous congested skin flap of rabbits 

Wang Chun-yan1, Tong Ting-hui2, Han Yin2, Wang Tai-ping2, Liao Yi2   

  1. 1Department of Anesthesiology; 2Plastic and Burn Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Received:2011-12-16 Revised:2012-01-05 Online:2012-04-29 Published:2012-04-29
  • Contact: Liao Yi, Professor, Chief physician, Plastic and Burn Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • About author:王春艳★，女，1976年生，吉林省长春市人，汉族， 2005年泸州医学院毕业，硕士，主治医师，主要从事生理学和临床麻醉学研究。tongth@163.com

PDF

359

被引次数

15

摘要：

背景：低分子肝素和水蛭素都有活血化淤作用。 
目的：观察局部注射低分子肝素或重组水蛭素对静脉淤血皮瓣成活的影响。
方法：在24只兔左耳背制作3 cm×6 cm静脉淤血皮瓣模型，随机分组：对照组皮瓣下多点注射生理盐水；肝素组皮瓣下多点注射低分子肝素625 U/mL，水蛭素组皮瓣下多点注射重组水蛭素1 U/mL。
结果与结论：①大体观察：术后水蛭素组和肝素组较对照组淤血程度轻，血肿消散快。②皮瓣成活面积百分比：水蛭素组、肝素组明显高于对照组(P < 0.01)，水蛭素组和肝素组比较差异无显著性意义(P > 0.05)。③苏木精-伊红染色结果：对照组术后同期微血管淤血程度较水蛭素组和肝素组严重，微血管计数明显少于水蛭素组和肝素组(P < 0.05，P < 0.01)，水蛭素组微血管计数明显高于肝素组(P < 0.01)。说明局部应用低分子肝素或重组水蛭素可较快改善皮瓣的淤血，提高皮瓣成活，重组水蛭素效果优于低分子肝素。
 

关键词: 重组水蛭素, 低分子肝素, 静脉淤血皮瓣, 微血管计数, 兔

Abstract:

BACKGROUND: Low-molecular-weight heparin (LH) and recombinant hirudin (RH) can promote blood circulation.
OBJECTIVE: To investigate the effect of local injection of LH or RH on the survival of venous congested skin flap．
METHODS: Twenty-four rabbits were employed to use the 3 cm×6 cm venous congested skin flap model on the back of left ear. The models were randomly divided into three groups: the control group was performed with multi-point injection of saline under skin flap; LH group was performed with multi-point injection of 625 U/mL LH under skin flap; RH group was multi-point injected with 1 U/mL RH under skin flap.
RESULTS AND CONCLUSION: ① General observation showed that the congestion in RH group and LH group was lighter than that in control group, and the hematoma dissipate quickly in RH group and LH group. ② The percentage of flap survival area in RH group and LH group was higher than that in the control group obviously (P < 0.01), there was no significant difference between RH group and LH group (P > 0.05). ③ Hematoxylin-eosin staining showed that congestion of microvascular in control group after operation was more serious than that in the RH group and LH group (P < 0.05, P < 0.01), the number of microvascular in RH group was higher than that in the LH group (P < 0.01). Local application of LH and RH can improve the congestion of skin flap, inhibit the survival rate of flap. There was better effect of using RH than LH. 

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