中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6841-6846.doi: 10.3969/j.issn.2095-4344.2012.37.001

• 骨组织构建 bone tissue construction •    下一篇

两种染色法判定左前臂骨缺损修复骨组织的成熟度

陶云霞，王根林，袁 鹏，施 琴，杨惠林   

  1. 苏州大学附属第一医院骨科，江苏省苏州市 215006
  • 收稿日期:2012-05-23 修回日期:2012-06-27 出版日期:2012-09-09 发布日期:2012-09-09
  • 通讯作者: 杨惠林，博士，教授，主任，苏州大学附属第一医院骨科，江苏省苏州市 215006 sdfyygk@163.com
  • 作者简介:陶云霞，女，1983年生，江苏省无锡市人，汉族，2008年江苏大学毕业，技师，主要从事骨科相关基础研究。 Taoyx8493@163.com

Determination of bone tissue maturity in repairing bone defects of the left forearm using two staining methods

Tao Yun-xia, Wang Gen-lin, Yuan Peng, Shi Qin, Yang Hui-lin   

  1. Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Received:2012-05-23 Revised:2012-06-27 Online:2012-09-09 Published:2012-09-09
  • Contact: Yang Hui-lin, Doctor, Professor, Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China sdfyygk@163.com
  • About author:Tao Yun-xia, Technician, Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China taoyx8493@163.com

PDF

768

被引次数

12

摘要：

背景：苏木精-伊红染色和Masson染色是常用的两种评价骨组织修复程度的方法，但上述两种方法能否有效地评价骨缺损修复过程中骨组织成熟度，目前尚缺乏相关研究。
目的：比较Masson染色和苏木精-伊红染色两种方法判定骨组织成熟度的敏感性。
方法：构建新西兰白兔15 mm桡骨缺损的动物模型，植入丝素蛋白/羟基磷灰石/骨髓基质干细胞的组织工程化骨，分别于术后第4，8，12周随机选取6只动物摄X射线平片，随后处死取材，进行大体标本观察、Masson染色及苏木精-伊红染色组织学观察。
结果与结论：X射线片与大体标本可见兔桡骨缺损逐渐修复，新生骨走向成熟骨。术后4周，苏木精-伊红染色在材料与骨的结合部及中间的材料上均形成大量新生软骨，有类骨样组织形成及有少量编织骨出现；Masson染色主要表现为绿色，可见到少许红染。术后8周，苏木精-伊红染色出现更多的新生骨组织，并更趋于成熟，出现了成熟的骨细胞及大量编织骨，有血管形成；Masson染色主要表现为红-绿相间。12周时，苏木精-伊红染色新生骨逐渐改建，形成髓腔，有大量板层骨相互融合成片；Masson染色主要表现为红色。说明Masson染色结合苏木精-伊红染色能较好判定新生骨走向成熟骨的过程，但在判定骨质成熟度时Masson染色较苏木精-伊红染色更直观。

关键词: 苏木精-伊红染色, Masson染色, 骨缺损, 骨髓基质干细胞, 丝素蛋白, 羟基磷灰石, 骨组织, 成熟度

Abstract:

BACKGROUND: Hematoxylin-eosin staining and Masson staining are two main methods that are commonly used for evaluating bone tissue repair, but there is a lack of the relevant research on whether these two methods can effectively evaluate the maturity of the bone tissue in the process of bone defect repair.
OBJECTIVE: To compare the sensitivity of Masson staining and hematoxylin-eosin staining on the determination of bone tissue maturity.
METHODS: New Zealand white rabbits were involved in the study for establishing models of radial bone defects (15 mm in length). The tissue engineered bone made up of silk fibroin/hydroxyapatite/bone marrow stromal cells was implanted into bone defects in rabbits. The X-ray plain films of six rabbits were randomly selected at 4, 8 and 12 weeks respectively after operation for the general observation, Masson staining and hematoxylin-eosin staining, then the animals were sacrificed.
RESULTS AND CONCLUSION: X-ray films and general specimens showed that bone defects were repaired and the new bone was changed into mature bone. At week 4, hematoxylin-eosin staining showed that a large number of new cartilages, some bone-like tissue and a small number of woven bones could be seen around the silk fibroin/hydroxyapatite grafts; Masson staining showed green with a little red dye. At week 8, a lot of new bone, mature osteoblasts and a large number of woven bones were observed by hematoxylin-eosin staining, and the blood vessel formation could be seen. The typical red-green and white views were observed by Masson staining. At week 12, hematoxylin-eosin staining showed that a large number of lamellar bones were integrated into a block, and the new medullary cavity could be seen; Masson staining showed mainly in red. Combination of hematoxylin-eosin staining and Masson staining is a better method to determine the process of new bone to mature bone. However, in the judgment of bone maturity, Masson staining is more intuitive than hematoxylin-eosin staining.

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