中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2509-2514.doi: 10.3969/j.issn.1673-8225.2012.14.008

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

自体骨髓间充质干细胞和同种异体软骨细胞共培养优化软骨组织工程种子的细胞源★

谢  鹏1，张仲文2   

  1. 1武警后勤学院附属医院骨科，天津市  300162；2武警总医院骨科，北京市  100010
  • 收稿日期:2011-10-15 修回日期:2012-01-17 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: author: Zhang Zhong-wen, Associate chief physician, Department of Orthoepdics, General Hospital of Armed Police Forces, Beijing 100010, China zzwen3@gmail.com
  • 作者简介:谢鹏★，男，1980年生，黑龙江省齐齐哈尔市人，汉族，2008年河北医科大学毕业，硕士，主治医师，主要从事关节外科的研究。xpxw2004@sina.com

Co-culture of autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes optimizes seed cell source of cartilage tissue engineering 

Xie Peng1, Zhang Zhong-wen2   

  1. 1Department of Orthopedics, Affiliated Hospital of Armed Police Logistics College, Tianjin  300162, China; 2Department of Orthoepdics, General Hospital of Armed Police Forces, Beijing  100010, China
  • Received:2011-10-15 Revised:2012-01-17 Online:2012-04-01 Published:2012-04-01
  • Contact: 张仲文，副主任医师，武警总医院骨科，北京市 100010 zzwen3@gmail.com
  • About author:Xie Peng★, Master, Attending physician, Department of Orthopedics, Affiliated Hospital of Armed Police Logistics College, Tianjin 300162, China xpxw2004@sina.com

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摘要：

背景：至今尚无一种细胞能完全满足组织工程对种子细胞的要求。
目的：探讨自体骨髓间充质干细胞和同种异体软骨细胞共培养的可行性。
方法：酶消化分离法获得兔软骨细胞，使用密度梯度离心和贴壁筛选的方法获得骨髓间充质干细胞。取细胞浓度为3×108 L-1的第2代骨髓间充质干细胞和软骨细胞，随机分为3组，共培养组：将骨髓间充质干细胞和软骨细胞按2∶1比例混匀；实验组：取同代同浓度的软骨细胞(浓度与共培养细胞的终浓度相同)；对照组：取低浓度软骨细胞1×108 L-1(与共培养组中软骨细胞终浓度相同)。
结果与结论：共培养组、实验组及对照组细胞平均群体倍增时间分别为3，7，8 d；共培养组共培养细胞增殖比其他2组明显增快(P < 0.05)；糖胺多糖水平明显多于其他2组(P < 0.05)；自体骨髓间充质干细胞与同种异体软骨细胞共培养未见明显排异反应，提示自体骨髓间充质干细胞与同种异体软骨细胞为种子细胞共培养，骨髓间充质干细胞能促进软骨细胞的增殖和细胞外基质合成，缩短软骨细胞培养时间和减少传代次数，同时软骨细胞可促进骨髓间充质干细胞向软骨细胞的定向转化，节省大量软骨细胞。
关键词：软骨细胞；共培养；骨髓间充质干细胞；组织工程；种子细胞
缩略语注释：BMSCs：bone marrow-derived mesenchymal stem cells，骨髓间充质干细胞
doi:10.3969/j.issn.1673-8225.2012.14.008

关键词: 软骨细胞, 共培养, 骨髓间充质干细胞, 组织工程, 种子细胞

Abstract:

BACKGROUND: There have been no cells that can meet the requirement of tissue engineering for seed cells.
OBJECTIVE: To investigate the feasibility of co-culturing autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes.
METHODS: Rabbit chondorcytes were harvested by enzymatic digestion. Bone marrow mesenchymal stem cells were harvested by density gradient centrifugation and adherence methods. Passage 2 bone marrow mesenchymal stem cells and chondrocytes at a concentration of 3×108/L were randomly divided into three groups. Co-culture group: bone marrow mesenchymal stem cells were co-cultured with chondrocytes at a ratio of 2: 1. Experimental group: Chondrocytes of the same passage and concentration (concentration the same as the final concentration of co-culture group); control group: 1×108 /L chondrocytes (concentration the same as the final concentration of co-culture group).
RESULTS AND CONCLUSION: The average population doubling time was 3 days in the co-culture group, 7 days in the experimental group, and 8 days in the control group. Cell proliferation was significantly faster in the co-culture group than in the experimental and control groups (P < 0.05). The glycosaminoglycan level was significantly higher in the co-culture group than in the experimental and control groups (P < 0.05). There was no obvious rejection in the co-cultures of autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes. This suggests that autogenic bone marrow mesenchymal stem cells in cultures can promote the proliferation of chondrocytes and the synthesis of extracellular matrix, shorten culture time of chondrocytes, and reduce passages; at the same time, chondrocytes can promote oriented transformation of bone marrow mesenchymal stem cells towards chondrocytes, which save a large number of chondrocytes.

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