中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2505-2508.doi: 10.3969/j.issn.1673-8225.2012.14.007

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

局部注射骨髓间充质干细胞和曲安奈德治疗兔跟腱炎的效果对比★

裘  超，洪建军，毛诚晃，卢晓郎   

  1. 温州医学院附属二院骨科，浙江省温州市  325000
  • 收稿日期:2011-12-16 修回日期:2012-03-03 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 洪建军，硕士，主任医师，副教授，硕士生导师。温州医学院附属二院骨科，浙江省温州市 325000 hjjwz@163.com
  • 作者简介:裘超★，男，1980年生，浙江省绍兴市人，汉族，温州医学院在读硕士，医师，主要从事骨科创伤的研究。 hynh24@yahoo.com.cn

Local injection of bone marrow mesenchymal stem cells versus triamcinolone acetonide for treatment of Achilles tendinitis in rabbits

Qiu Chao, Hong Jian-jun, Mao Cheng-huang, Lu Xiao-lang   

  1. Department of Orthopedics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou  325000, Zhejiang Province, China
  • Received:2011-12-16 Revised:2012-03-03 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Hong Jian-jun, Master, Chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China hjjwz@163.com
  • About author:Qiu Chao★, Studying for master’s degree, Physician, Department of Orthopedics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China hynh24@yahoo.com.cn

PDF

348

被引次数

2

摘要：

景：应用长效糖皮质激素局部封闭治疗跟腱炎在学术界一直很有争议。
目的：对跟腱炎模型兔跟腱局部分别注射兔骨髓间充质干细胞和长效糖皮质激素曲安奈德，动态观察相关指标，观察骨髓间充质干细胞和曲安奈德对跟腱炎的组织结构和力学性能的影响。
方法：将60只单侧跟腱炎新西兰大白兔模型随机等分4组，取其中2组分别于患侧跟腱局部注射骨髓间充质干细胞和曲安奈德1次；剩余2组则分别注射2次，注射间隔2周。
结果与结论：相比1次曲安奈德组，局部注射骨髓间充质干细胞1次和2次的跟腱炎模型兔跟腱腱束整齐、致密，断裂少，成纤维细胞增多，跟腱组织羟脯氨酸含量、跟腱最大负荷拉力均增加(P < 0.05)，2次注射的治疗效果更明显(P < 0.05)。说明局部重复注射骨髓间充质干细胞移植治疗跟腱炎的效果优于长效糖皮质激素曲安奈德。

关键词: 跟腱炎, 动物模型, 骨髓间充质干细胞, 曲安奈德, 局部注射, 兔

Abstract:

BACKGROUND: It remains to be disputed to apply long-lasting glucocorticoid for partial closure theray of Achilles tendonitis.
OBJECTIVE: Bone marrow mesenchymal stem cells and long-lasting triamcinolone acetonide were respectively injected into the achilles tendon of a rabbit model of Achilles tendonitis to dynamically observe related indices and investigate the effects of bone marrow mesenchymal stem cells and long-lasting triamcinolone acetonide on tissue structure and mechanical performance of Achilles tendon with tendonitis.
METHODS: Sixty rabbit models of unilateral Achilles tendinitis were randomly divided into four groups. Two groups were locally administered bone marrow mesenchymal stem cells and triamcinolone acetonide respectively once, and the remaining two groups were locally administered bone marrow mesenchymal stem cells and glucocortocoid respectively twice with a time interval of 2 weeks.
RESULTS AND CONCLUSION: Compared with triamcinolone acetonide once group, rabbits in the bone marrow mesenchymal stem cells once or twice groups, rabbits exhibited regular, dense tendon bundles, increased fibroblasts, increased hydroxyproline content in the tendon tissue and increased maximum load strength of tendon (P < 0.05), in particular twice injections (P < 0.05). These findings suggest that local repeated injections of bone marrow mesenchymal stem cells exhibit better therapeutic effects than long-lasting triamcinolone acetonide application in treatment of Achilles tendinitis.

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