中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7572-7579.doi: 10.3969/j.issn.2095-4344.2012.40.029

• 器官移植学术探讨 academic discussion of organ transplantation • 上一篇    下一篇

肾移植后巨细胞病毒感染的检测

金春明   

  1. 牡丹江医学院红旗医院检验科，黑龙江省牡丹江市 157000
  • 收稿日期:2012-06-18 修回日期:2012-07-25 出版日期:2012-09-30 发布日期:2012-09-30
  • 作者简介:金春明，女，1970年生，牡丹江市人，朝鲜族，1992年牡丹江医学院毕业，副主任检验师，主要从事临床免疫、生化、分子生物学检验研究。 jcm-1234@163.com

Detection of cytomegalovirus infection after renal transplantation

Jin Chun-ming   

  1. Department of Laboratory, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157000, Heilongjiang Province, China
  • Received:2012-06-18 Revised:2012-07-25 Online:2012-09-30 Published:2012-09-30
  • About author:Jin Chun-ming, Associate chief examiner, Department of Laboratory, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157000, Heilongjiang Province, China jcm-1234@163.com

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442

被引次数

3

摘要：

背景：肾移植后因患者细胞免疫、体液免疫均明显下降，易发生各种类型感染，其中以巨细胞病毒感染多见，且有较高的发病率和病死率，早期准确的检测有利于指导临床治疗。
目的：对肾移植后巨细胞病毒感染检测研究的文献资料进行多层次的探讨分析。
方法：以电子检索的方式对CNKI数据库2002-01/2011-12 有关肾移植后巨细胞病毒感染检测研究的文献进行分析，采用检索词为“肾移植(renal transplantation)；巨细胞病毒(cytomegalovirus)；BK病毒(BK virus)；疱疹病毒(herpes virus)”。巨细胞病毒是肾移植后感染的主要病原体，与肾移植后肺炎、肝炎、视网膜炎及肾移植后急、慢性排斥反应的发生有着密切的联系。
结果与结论：肾移植后巨细胞病毒感染的检测方法不断改进，检测方法较多，目前尚无一种理想的方法可以早期及时准确检测出活动性巨细胞病毒感染，主要是将血清学检测、荧光免疫标记检测、抗原抗体检测以及基因检测进行相结合来检测诊断肾移植后巨细胞病毒感染，尤其是巨细胞病毒晚期抗原(pp65)检测迅速发展，提高了巨细胞病毒感染检测的特异性及敏感性，为临床诊断及预防治疗提供了重要的参考依据。

关键词: 肾移植, 巨细胞病毒, BK病毒, 疱疹病毒, 感染, 肺部感染, 检测, 晚期抗原, 抗原, 抗体

Abstract:

BACKGROUND: Due to the decreasing cellular immunity and humoral immunity after renal transplantation, the patients may suffer various types of infection easily, and cytomegalovirus infection is more common with high morbidity and mortality. The early and accurate detection is conducive to guide clinical treatment.
OBJECTIVE: To multi-level explore and analyze the articles on the detection of cytomegalovirus infection after renal transplantation.
METHODS: A computer-based search was performed in CNKI database from January 2002 to December 2011 for the articles on the detection of cytomegalovirus infection after renal transplantation. The key words were “renal transplantation, cytomegalovirus, BK virus” in Chinese and English. Cytomegalovirus was the main pathogens for the infection after renal transplantation, and was closely related with the occurrence of pneumonia and hepatitis after renal transplantation, retinitis and the acute and chronic rejection after renal transplantation.
RESULTS AND CONCLUSION: The method for the detection of cytomegalovirus infection after renal transplantation is continuously improved and there are various detection methods. To data, there is no ideal way to early and accurate detect the active cytomegalovirus infection. The method for diagnosing the cytomegalovirus infection after renal transplantation is mainly the combination of serological testing, fluorescence immunoassay mark detection, detection of antigen-antibody and genetic testing. Detection of the cytomegalovirus late antigen (pp65) is developed rapidly, which improve the specificity and sensitivity of detection of cytomegalovirus infection, and provide an important reference for the clinical diagnosis and preventive treatment.

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