中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7751-7754.doi: 10.3969/j.issn.1673-8225.2011.41.038

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

骨形态发生蛋白9的成骨效应研究与进展

江  维，胡侦明，郝  杰，刘  渤，甘  强   

  1. 重庆医科大学附属第一医院，重庆市  400016
  • 收稿日期:2011-04-17 修回日期:2011-06-17 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 胡侦明，博士，教授，重庆医科大学附属第一医院，重庆市 400016
  • 作者简介:江维，男，1985年生，重庆市人，汉族，主要从事骨生长因子、 骨再生修复的组织工程学方面的研究。 390373532@ qq.com

Research progress in osteogenic effects of bone morphogenetic protein 9

Jiang Wei, Hu Zhen-ming, Hao Jie, Liu Bo, Gan Qiang   

  1. First Hospital Affiliated to Chongqing Medical University, Chongqing  400016, China
  • Received:2011-04-17 Revised:2011-06-17 Online:2011-10-08 Published:2011-10-08
  • Contact: Hu Zhen-ming, Doctor, Professor, First Hospital Affiliated to Chongqing Medical University, Chongqing 400016, China
  • About author:Jiang Wei, First Hospital Affiliated to Chongqing Medical University, Chongqing 400016, China 390373532@qq.com

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349

被引次数

3

摘要：

背景：组织工程人工骨是解决传统植骨骨量不足，新骨形成缓慢的有效途径，而寻找促进细胞增殖和成骨分化的因子和技术是目前研究组织工程骨的关键领域。
目的：全面了解骨形态发生蛋白9的成骨机制、作用及表达纯化的方法，为更好的实现成骨分化因子在临床促进骨形成，解决骨缺损修复奠定基础。
方法：检索PubMed数据库2000-01/2010-12及CNKI数据库2006-01/2010-12收录的骨形态发生蛋白9相关综述和论文报告，并分析其成骨机制、作用及表达纯化的方法几个方面的研究进展。
结果与结论：共纳入骨形态发生蛋白9的相关文献21篇。在骨形态发生蛋白9诱导成骨过程中，经典Wnt信号通路、Hey1、碱性螺旋-环-螺旋蛋白和过氧化物酶增殖体活化受体γ2、激活素受体样激酶1和2、胰岛素生长因子2及类维生素A等机制都起着重要的作用。目前，骨形态发生蛋白9已经在体内外被证实是成骨能力最强的转化生长因子。对骨形态发生蛋白9的研究主要是通过重组腺病毒作为载体，但目前构建的病毒载体会有许多编码蛋白质的基因，其表达产物免疫原性很强，应用上存在安全隐患。有学者将真核质粒作为载体，转染真核细胞中，得到骨形态发生蛋白4。能否用此方法得到骨形态发生蛋白9，从而解决腺病毒应用的安全性问题，为临床治疗骨缺损等提供生物技术支持，还有待进一步的研究证实。

关键词: 骨形态发生蛋白9, 成骨, 表达, 纯化, 修复

Abstract:

BACKGROUND: Tissue-engineered artificial bone is an effective way to solve the bone deficiency of traditional bone grafting, and the slow formation of new bones. Furthermore, looking for the factors and technology which promote cell proliferation and osteogenic differentiation is a key of current study on tissue engineering bone.
OBJECTIVE: To overall understand the osteogenic mechanism, function and expression and purification methods of bone morphogenetic protein 9 (BMP-9) and to lay the foundation for better implementation of osteogenic differentiation factor in clinical bone formation and repair bone defects.
METHODS: A computer-based retrieval was performed in PubMed database (2000-01/2010-12) and CNKI database (2006-01/2010-12) to search review, reports regarding BMP-9. The osteogenic mechanism, function and expression and purification of BMP-9 were analyzed.
RESULTS AND CONCLUSION: Twenty-one papers regarding BMP-9 were included. In the process of bone formation induced by BMP-9, classical Wnt signaling pathway, Hey 1, basic Helix-loop-helix protein, peroxidase of proliferation activation receptor-gamma-2, activin receptor-like kinase 1 (ALK1), ALK2, insulin-like growth factor-2, type of vitamin A and other mechanisms play an important role. At present, BMP-9 has proven to be the transforming growth factor that has the strongest osteogenic potential in vivo and in vitro. Studies on BMP-9 mainly taking recombinant adeno-associated virus as a vector, but the current construction of the virus vectors will have many genes of the proteins encoded, whose expression products have strong immunogenicity, and the application has security risks. Some scholars used eukaryotic plasmid as vector and transfected it into enkaryotic cells and obtained bone morphogenetic protein-4. But whether this method can be used to produce BMP-9 and to solve the adenovirus-application security issues, and provide clinical treatment of bone defect with biotechnical support, still requires further study.

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