中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5801-5804.doi: 10.3969/j.issn.1673-8225.2011.31.024

• 移植与基因 transplantation and gene • 上一篇    下一篇

河南省骨髓库汉族捐献者HLA-A、B、DR高分辨基因多态性调查分析

王兆福，张伯伟，马 茹，杜  娟，孔大为   

  1. 河南省红十字血液中心，河南省郑州市  450012
  • 收稿日期:2011-02-11 修回日期:2011-03-14 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 张伯伟，主任技师，河南省红十字血液中心，河南省郑州市 450012
  • 作者简介:王兆福，男，1961年生，河南省南阳市人，汉族，1984年中山医科大学医学系毕业，副教授，主要从事输血医学教学科研工作。 659014888w@ sina.com

HLA-A, B and DR high-resolution gene polymorphism of Henan Stem Cell Registry 

Wang Zhao-fu, Zhang Bo-wei, Ma Ru, Du Juan, Kong Da-wei   

  1. Henan Red Cross Blood Center, Zhengzhou   450012, Henan Province, China
  • Received:2011-02-11 Revised:2011-03-14 Online:2011-07-30 Published:2011-07-30
  • Contact: Zhang Bo-wei, Chief technician, Henan Red Cross Blood Center, Zhengzhou 450012, Henan Province, China
  • About author:Wang Zhao-fu, Associate professor, Henan Red Cross Blood Center, Zhengzhou 450012, Henan Province, China 659014888w@sina. com

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被引次数

22

摘要：

背景：2009年开始，中华骨髓库加大HLA高分检测的力度，可缩短配型检索周期，提高配型成功率。
目的：统计分析河南省骨髓捐献者HLA-A、B、DRB1高分辨基因多态性。
方法：河南省汉族造血干细胞志愿捐献者血样3 874人份，志愿捐献静脉血，EDTA抗凝。采用SSO HD 荧光微珠流式高分辨HLA-A、B、DRB1基因分型检测方法，对3 874份骨髓捐献志愿者血样进行高分辨分型检测，用PCR-SSP高分辨检测、SBT检测解决存在的模棱两可问题。等位基因计数法计算等位基因的基因频率。
结果与结论：共检出A 34个，B 84个，DRB1 50个，A位点基因频率处于前5位的有A*0201(0.160 9)、 A*1101(0.162 7)、 A*2402(0.160 2)、A*3001(0.080 8)、 A*3303(0.078 9)；B位点处于前5位的是B*1302(0.077 7)、B*4001(0.072 2)、B*5101(0.062 8)、B*4601(0.061 2)和B*0702(0.050 7)；DRB1位点处于前5位的是DRB1*1501(0.146 9)、DRB1*0901(0.131 8)、DRB1*0701(0.121 1)、DRB1*1202(0.061 2)、DRB1*0405(0.058 2)。

关键词: 中华骨髓库, HLA 高分辨, 基因频率, 多态性, 河南省

Abstract:

BACKGROUND: Since 2009, Chinese Marrow Donor Program takes more efforts in high resolution HLA typing, shortens typing retrieval time and increases the success rate of HLA typing.
OBJECTIVE: To statistically analyze HLA-A, B, DRB1 high-resolution gene polymorphism in bone marrow donors from Henan Province, China.
METHODS: A total of 3 874 venous blood samples were collected from the volunteer donors and subjected to ethylenediamine tetraacetic acid anticoagulation. The blood samples were detected using PCR-SSO HD high-resolution HLA typing method (LABType ® HD, Onelambda USA). The SSP and SBT detection tests were performed to solve ambiguity. Allele frequency was calculated with the direct count method.
RESULTS AND CONCLUSION: A total of 168 alleles were detected, 34 of which were for HLA-A, 84 for B, and 50 for DRB1. The top five loci for each was A*0201(0.160 9), A*1101 (0.162 7), A*2402(0.160 2), A*3001(0.080 8), A*3303 (0.078 9); B*1302(0.077 7), B*4001(0.072 2), B*5101(0.062 8), B*4601(0.061 2), B*0702(0.050 7); DRB1*1501(0.146 9), DRB1*0901(0.131 8 ), DRB1*0701(0.121 1), DRB1*1202(0.061 2), DRB1*0405(0.058 2).

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