中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4638-4642.doi: 10.3969/j.issn.1673-8225.2011.25.019

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

富血小板凝胶与拔牙窝新骨形成和骨量保持

王承勇，卢  萌，林  海，陈伟辉   

  1. 福建医科大学附属协和医院口腔颌面外科，福建省福州市 350001
  • 收稿日期:2011-03-25 修回日期:2011-05-01 出版日期:2011-06-18 发布日期:2014-01-10
  • 通讯作者: 陈伟辉，博士，硕士生导师，主任，副教授，福建医科大学附属协和医院口腔颌面外科，福建省福州市 350001
  • 作者简介:王承勇☆，男，1980年生，福建省福清市人，汉族，2003年福建医科大学毕业，硕士，医师，主要从事口腔种植修复方面的研究。 yulong5270@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(批准号：31070838)，课题名称：交感神经系统信号在生理咬合力调节骨改建的作用机制。国家教育部留学回国人员科研启动基金：影响富血小板血浆活性相关因素分析及其对颅骨细胞粘附、迁移及体外矿化的调节作用。

Effect of platelet-rich gel on new bone formation and bone maintenance following tooth extraction

Wang Cheng-yong, Lu Meng, Lin Hai, Chen Wei-hu   

  1. Department of Oral and Maxillofacial Surgery, Union Hospital, Fujian Medical University, Fuzhou   350001, Fujian Province, China
  • Received:2011-03-25 Revised:2011-05-01 Online:2011-06-18 Published:2014-01-10
  • Contact: Chen Wei-hui, Doctor, Master’s supervisor, Associate professor, Department of Oral and Maxillofacial Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China
  • About author:Wang Cheng-yong★, Master, Physician, Department of Oral and Maxillofacial Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China yulong5270@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 31070838; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry*

PDF

315

被引次数

9

摘要：

背景：富血小板血浆能否促进骨组织的修复再生存在一定的争议。
目的：研究富血小板血浆/凝胶对拔牙窝骨愈合过程中新骨形成和骨量保持的可能调节作用，探讨富血小板血浆/凝胶与骨愈合的相互关系。
方法：通过拔牙建立Beagle犬拔牙窝骨缺损模型，同期在拔牙窝导入富血小板血浆或复血小板凝胶，并设计对照组。术后2，4，8，12周分别进行大体观察、放射影像学检查、三维CT平扫+重建、组织学检查拔牙窝颊舌侧牙槽嵴高度差、CT值以及新生骨面积。
结果与结论：与富血小板血浆组和对照组比较，影像学结果表明富血小板凝胶组在第2，4，8周新骨形成的面积最大(P < 0.01)；组织学结果表明富血小板凝胶组在第2，4周新骨形成面积最大(P < 0.05)；在所有时间点上富血小板凝胶组颊舌侧牙槽嵴高度差值最小(P < 0.05)。在第12周，富血小板凝胶组颊舌侧牙槽嵴高度仍有2 mm差值。提示，富血小板凝胶具有促进牙槽窝早期愈合的能力，但其单独使用时促进牙槽窝骨量保持的效能有限。

关键词: 富血小板血浆, 富血小板凝胶, 拔牙窝, 骨愈合, 放射影像学

Abstract:

BACKGROUND: Whether platelet-rich plasma can promote bone tissue regeneration or not is controversial.
OBJECTIVE: To explore the effect of platelet-rich plasma (PRP)/platelet-rich gel (PRG) on bone healing after tooth extraction by an animal model of denervation.
METHODS: The premolars of both sides were extracted from Beagle dogs, then a filling socket with PRP or PRG was introduced. The Beagle dogs were sacrificed at 2, 4, 8, 12 weeks. X-ray, CT scan, hematoxylin-eosin staining were used to detect the effect of PRP or PRG on the new bone formation and maintenance after tooth extraction.
RESULTS AND CONCLUSIOU: CT scan convinced that compared with group PRP and control, group PRG had more new bone at 2, 4, 8 weeks. While, histological analysis showed that only at 2,4 weeks, group PRG had the advantage of osteogenesis. At all time points, group PRG showed the lest difference of buccal –lingual height. At 12 weeks, the difference of buccal and lingual height in group PRG was still 2mm.PRG had the ability to promote early phase healing of extraction sockets. PRG showed the limited efficiency in bone maintenance when used exclusively after extraction.

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