中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2609-2612.doi: 10.3969/j.issn.1673-8225.2011.14.030

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

骨髓增生异常综合征患者SFRP5基因启动子区甲基化状态研究

史晓红1，范  芸2，周昌虎1，宁尚勇2，孙  亮1，朱小泉1，唐  雷1，常乃柏2，杨  泽1   

  1. 卫生部北京医院，1卫生部北京老年医学研究所，2血液科，北京市100730
  • 收稿日期:2010-11-23 修回日期:2011-01-25 出版日期:2011-04-02 发布日期:2013-11-02
  • 通讯作者: 杨泽，研究员，卫生部北京医院，卫生部北京老年医学研究所，北京市100730 yangze016@yahoo.com.cn 并列通讯作者：常乃柏，主任医师，卫生部北京医院血液科，北京市100730 changnaibai@sohu.com
  • 作者简介:史晓红☆，女，1975年生，河北省石家庄人，汉族，2009年中国科学院遗传与发育生物学研究所毕业，博士，副研究员，主要从事医学遗传学研究。 sxh_cnu@yahoo.com.cn 并列第一作者：范芸，女，1968年生，山东省淄博市人，汉族，2000年协和医科大学毕业，硕士，主任医师，主要从事临床血液病诊治工作。 emmafan@sina.com.cn
  • 基金资助:

    卫生部北京医院重点基金（BJ-2008-76），课题名称：急性非淋巴细胞白血病的分子诊断学研究。卫生部北京医院面上基金（BJ-2008-110），课题名称：三氧化二砷作用下骨髓增生异常综合征细胞株MUTZ-1JAK2/STAT5基因表达的变化。

Methylation status in the promoter region of secreted frizzled related protein 5 gene in patients with myelodyplastic syndrome

Shi Xiao-hong1, Fan Yun2, Zhou Chang-hu1, Ning Shang-yong2, Sun Liang1, Zhu Xiao-quan1, Tang Lei1, Chang Nai-bai2, Yang Ze1   

  1. 1Institute of Geriatrics, 2Department of Hematology, Ministry of Public Health, Beijing Hospital, Beijing  100730, China
  • Received:2010-11-23 Revised:2011-01-25 Online:2011-04-02 Published:2013-11-02
  • Contact: Yang Ze, Researcher, Institute of Geriatrics, Ministry of Public Health, Beijing Hospital, Beijing 100730, China yangze016@yahoo.com.cn Correspondence to: Chang Nai-bai, Chief physician, Department of Hematology, Ministry of Public Health, Beijing Hospital, Beijing 100730, China changnaibai@sohu.com
  • About author:Shi Xiao-hong☆, Doctor, Associate investigator, Institute of Geriatrics, Ministry of Public Health, Beijing Hospital, Beijing 100730, China sxh_cnu@yahoo.com.cn Fan Yun, Master, Chief physician, Department of Hematology, Ministry of Public Health, Beijing Hospital, Beijing 100730, China emmafan@sina.com.cn Shi Xiao-hong and Fan Yun contributed equally to this paper.
  • Supported by:

    the Key Foundation of Beijing Hospital, Ministry of Public Health, No. BJ-2008-76*; General Foundation of Beijing Hospita Ministry of Public Health l, No. BJ-2008-110*

PDF

398

被引次数

15

摘要：

背景：SFRP5基因启动子区的甲基化与急性髓系白血病的发生密切相关。作为白血病的前期，骨髓增生异常综合征患者中SFRP5基因启动子区的甲基化状态尚不清楚。
目的：探索骨髓增生异常综合征中WNT/β-catenin信号传导通路拮抗基因分泌型卷曲相关蛋白5(SFRP5)基因的甲基化状态。
方法：应用甲基化特异性PCR(MSP)法对43例骨髓增生异常综合征患者的骨髓或外周血进行SFRP5基因启动子区甲基化状况检测，并以70例门诊普通患者的外周血作为对照。
结果与结论：113例样本均成功进行了MSP检测，进入结果分析。43例骨髓增生异常综合征患者中检出5例(11.6%)SFRP5的甲基化，70例正常对照中检出1例(1.4%)SFRP5的甲基化，并且均为不完全甲基化状态。SFRP5在骨髓增生异常综合征患者中的甲基化率显著高于正常对照(χ2=5.511，P=0.019)。43例患者样本中，17例为骨髓样本，26例为外周血样本。而5例甲基化的样本中，2例为骨髓样本，3例为外周血样本。样本的来源(骨髓/外周血)对于甲基化状态的结果无显著差异(χ2=0.001，P=0.982)。提示，SFRP5基因的甲基化与骨髓增生异常综合征有相关性，SFRP5基因的甲基化可能是引起骨髓增生异常综合征的分子机制之一。

关键词: 骨髓增生异常综合征, SFRP5基因, 甲基化, 外周血, 对照

Abstract:

BACKGROUND: Methylation in the promoter region of secreted frizzled related protein 5 (SFRP5) gene is closely related to acute myeloid leukemia. As a pre-leukemia, methylation status in the promoter region of SFRP5 gene in patients with myelodyplastic syndrome (MDS) remains unclear.
OBJECTIVE: To investigate the methylation status in the promoter region of SFRP5 gene in patient with MDS.
METHODS: Methylation specific PCR (MSP) method was applied to examine the promoter methylation of SFRP5 gene in 43 bone marrow or peripheral blood samples of MDS patients, and 70 normal peripheral blood samples from volunteers of general outpatients as controls.
RESULTS AND CONCLUSION: All 113 samples were involved for the results analysis. In 43 patients of MDS, 5 samples (11.6%) showed SFRP5 gene methylation. In 70 controls, 1 sample showed SFRP5 gene methylation. And all of them were part-methylation status. The frequency of SFRP5 gene methylation in MDS patients was significantly higher than that in controls (χ2=5.511, P=0.019). Of the 43 MDS patients’ samples, 17 cases were bone marrow samples, 26 cases were peripheral blood samples. Of the 5 SFRP5 gene methylation samples, 2 cases were bone marrow samples and 3 cases were peripheral blood samples. There was no significant difference between the different sample source (bone marrow or peripheral blood ) for the results of the methylation status (χ2=0.001, P=0.982). The methylation status of SFRP5 gene was correlated with MDS. The methylation of SFRP5 gene may be one of the molecular mechanisms that contributes to the prognosis of patients with MDS.

中图分类号: