中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1235-1238.doi: 10.3969/j.issn.1673-8225.2010.07.022

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

微波热凝后骨骼肌体积及功能的变化

张晓旻，武和明，邢树忠   

  1. 南京医科大学口腔医学院，江苏省南京市  210029
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 通讯作者: 邢树忠，硕士，教授，南京医科大学口腔医学院，江苏省南京市 210029
  • 作者简介:张晓旻★，女，1973年生，江苏省南京市，汉族，2003年南京医科大学毕业，硕士，讲师，主要从事口腔医学基础与临床研究。 Zhangxiaomin2002@yahoo.com.cn

Volumetric and functional changes of skeletal muscles following microwave coagulation

Zhang Xiao-min, Wu He-ming, Xing Shu-zhong   

  1. Stomatological College of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Xing Shu-zhong, Master, Professor, Stomatological College of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
  • About author:Zhang Xiao-min★, Master, Lecturer, Stomatological College of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China Zhangxiaomin2002@yahoo.com.cn

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摘要：

背景：微波利用其热效应在医学领域得到广泛应用，经微波热凝作用后造成局部组织凝固性坏死，坏死部位由纤维组织修复。但是微波热凝是否能产生缩容效果运用于口腔临床，目前经检索国内外尚未见报道。
目的：观察微波热凝骨骼肌后肌肉体积和功能的改变，探讨微波热凝用于骨骼肌体积缩小的可能性。　
方法：20 只新西兰大白兔暴露双侧胫骨前肌，采用2 450 MHz微波治疗仪以70 W微波热凝一侧胫骨前肌20 s，另一侧不做微波热凝作对照。分别于热凝后24，48 h和1，8周，随机各处死5只兔，测量双侧胫前肌体积变化，8周处死动物前，行肌电生理检测双侧胫前肌的肌动力。　
结果与结论：微波热凝后24，48 h骨骼肌体积增大[(5.82±0.93)，(6.04±0.47) mL]，48 h体积最大，1周后肌肉体积开始缩小[(4.90±0.80) mL]，8周后体积[(4.27±0.67) mL]缩小23.6%；肌电生理检测显示对照组与热凝组潜伏期分别为(1.765±0.393)，(1.760±0.394) ms，波宽分别为(6.273±0.808)，(6.259±0.773) ms，两组之间传导速度及波宽差异无显著性意义(P > 0.05)。结果证实微波热凝后48 h内肌肉体积增大，随后体积减小，骨骼肌可保持肌功能。

关键词: 微波热凝, 肌动力, 骨骼肌, 体积, 肌肉及肌腱组织工程

Abstract:

BACKGROUND: Microwave had been widely used in medical field, which can lead to local coagulation necrosis and repair the necrosis with fibrous tissue. However, whether microwave coagulation can be used in stomatology is poorly understood. 
OBJECTIVE: To observe the functional and volumetric changes of skeletal muscles following microwave coagulation, and to explore the possibility of microwave coagulation for volumetric reduction of skeletal muscle.
METHODS: Both sides of tibialis anterior muscle of 20 New Zealand rabbits were exposed; one side was coagulated by
2 450 MHz microwave therapeutic instrument at 70 W for 20 seconds. No treatment was performed at the other side. Rabbits were sacrificed at hours 24, 48, weeks 1 and 8 after microwave coagulation. The volumetric changes of the ablated tibialis anterior muscle were measured, and electricitic physiology observations were conducted on the ablated muscle at 8 weeks after microwave coagulation before being sacrificed. 　
RESULTS AND CONCLUSION: The volume of ablated muscle increased at hours 24 and 48, which was (5.82±0.93) and (6.04±0.47) mL, especially greater at hour 48 after microwave coagulation. After 1 week, the muscle volume began to decrease to (4.90±0.80) mL, reduced to (4.27±0.67) mL at week 8, which was 23.6% volumetric loss. However, the electrophysiologic observation showed that the latent periods were (1.765±0.393) and (1.760±0.394) ms, and the wave width was (6.273±0.808) and (6.259±0.773) ms of the control group and experimental group, respectively, without apparent differences (P > 0.05). The volume of the skeleton muscle increased at hour 48 after microwave coagulation, and then decreased, but the muscle function of the skeleton muscle can be preserved.

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