中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 317-320.doi: 10.3969/j.issn.1673-8225.2011.02.031

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

不同运动方式和时间对大鼠骨骼肌线粒体呼吸链复合体酶Ⅰ和Ⅳ活性的影响

郭彦青1，张  敏2，赵晓丽1，王丽艳1，曹  颖1，于公元1，李海东1   

  1. 1天津医科大学生物化学系，天津市300070
    2武装警察部队医学院生物化学系，天津市 300162
  • 收稿日期:2010-08-17 修回日期:2010-09-26 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 李海东，博士，教授，天津医科大学生物化学系，天津市300070 HL@tijmu.edu.cn
  • 作者简介:郭彦青★，女，1981年生，山西省原平市人，汉族，天津医科大学在读硕士，主要从事生物化学方面的研究。 9754074@qq.com

Effects of different exercise time and methods on the activities of mitochondrial respiratory chain complexes Ⅰ and Ⅳ from rat skeletal muscles

Guo Yan-qing1, Zhang Min2, Zhao Xiao-li1, Wang Li-yan1, Cao Ying1, Yu Gong-yuan1, Li Hai-dong1   

  1. 1Department of Biochemistry, Tianjin Medical University, Tianjin  300070, China
    2Department of Biochemistry, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2010-08-17 Revised:2010-09-26 Online:2011-01-08 Published:2011-01-08
  • Contact: Li Hai-dong, Doctor, Professor, Department of Biochemistry, Tianjin Medical University, Tianjin 300070, China HL@tijmu.edu.cn
  • About author:Guo Yan-qing★, Studying for master’s degree, Department of Biochemistry, Tianjin Medical University, Tianjin 300070, China 9754074@qq.com

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619

被引次数

30

摘要：

背景：急性剧烈运动对机体可造成氧化应激，线粒体生成活性氧造成机体氧化应激和钙离子超负荷，导致骨骼肌功能下降，机体产生疲劳。然而长期规律运动后线粒体产生适应性变化，从而起到保护机体免受过多活性氧损害的作用。
目的：观察不同运动方式和时间对大鼠骨骼肌线粒体呼吸链复合体酶Ⅰ，Ⅳ活性的影响。
方法：48只健康雄性SD大鼠随机等分为正常对照组、无氧运动组、有氧运动组和交替运动组。在大鼠运动2，4，6周的时候分别处死，差速离心提取大鼠骨骼肌线粒体，分光光度法测定线粒体呼吸链复合体酶Ⅰ，Ⅳ的活性。
结果与结论：运动后有氧组、交替组与无氧组线粒体呼吸链复合体酶I的活性随时间先增强而后下降；运动后有氧组与交替组线粒体呼吸链复合体酶Ⅳ的活性随时间而增强，无氧组则下降。说明有氧运动在中长期耐力运动中可有效提高大鼠体内呼吸链复合体酶Ⅰ，Ⅳ的活性，而无氧运动则只能在短期内提高其活性，长时间无氧运动可能导致对线粒体呼吸链复合体酶的损伤，影响骨骼肌工作效率，造成机体疲劳。

关键词: 运动方式, 运动时间, 骨骼肌, 线粒体, 呼吸链复合体酶

Abstract:

BACKGROUND: Acute violent exercise can result in oxidative stress reaction. Reactive oxygen species mitochondrioa cause oxidative stress and calcium ion over-loading, leading to the function of skeletal muscle and body fatigue. However, the mitochondra can have adaptive changes following long-term regular exercise, which can protect body from damage of over-produced reactive oxygen species.   
OBJECTIVE: To investigate the effects of different exercise time and methods on the activities of skeletal muscle mitochondrial respiratory chain complexesⅠ and Ⅳ.
METHODS: A total of 48 healthy male SD rats were randomly divided into normal control, anaerobic, alternating and aerobic exercise groups. After 2, 4 and 6 weeks of exercising, the rats were sacrificed and skeletal muscle mitochondria were isolated by differential centrifugation. The activities of mitochondrial respiratory chain complexes Ⅰ and Ⅳ were measured by spectrophotometry.
RESULTS AND CONCLUSION: The activitie of complexes Ⅰ and Ⅳ of the aerobic and alternating exercise groups increased with time after exercising, while those of the anaerobic group were increased before 6 weeks, and then decreased afterwards. The results suggest that aerobic exercise can enhance the activities of rat mitochondrial respiratory chain complexes Ⅰ and Ⅳ during endurance exercise. Anaerobic exercise can enhance the activities only in short term. Longer anaerobic exercise may damage mitochondrial complexes, resulting in decreased energy generation and body fatigue.

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