中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9933-9936.doi: 10.3969/j.issn.1673-8225.2010.53.013

• 肾移植 kidney transplantation • 上一篇    下一篇

大鼠双侧供肾左侧原位肾移植模型的建立及肾功能监测　

方  成1，刘  立1，胡汉宁2，陈  薇2，王加谋1，张奇峰1，瞿永华1　   

  1. 1 武汉工业学院移植工程实验室，湖北省武汉市 430023；2 武汉大学中南医院检验科，湖北省武汉市 430071
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 作者简介:方成☆，男，1972年生，湖北省武汉市人，汉族，2003年武汉大学毕业，博士，副教授，主要从事组织及器官移植免疫与材料方向的研究。 fang_cheng@yahoo.cn
  • 基金资助:

    湖北省自然科学基金课题(2008CDB150)，课题名称：大鼠肾脏组织蛋白在异体移植排斥反应中的识别和应答。

Establishment of left kidney transplantation models in rats with bilateral kidney grafts and monitoring of kidney functions

Fang Cheng1, Liu Li1, Hu Han-ning2, Chen wei2, Wang Jia-mou1, Zhang Qi-feng1, Qu Yong-hua1   

  1. 1 Laboratory of Transplantation Engineering, Wuhan Polytechnical University, Wuhan  430023, Hubei Province, China; 2 Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan  430071, Hubei Province, China
  • Online:2010-12-31 Published:2010-12-31
  • About author:Fang Cheng☆, Doctor, Associate professor, Laboratory of Transplantation Engineering, Wuhan Polytechnical University, Wuhan 430023, Hubei Province, China fang_cheng@yahoo.cn
  • Supported by:

    the Natural Science Foundation of Hubei Province, No. 2008CDB150*

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摘要：

背景：大鼠肾移植模型是移植免疫耐受、免疫抑制及器官库开发的重要实验手段。
目的：建立实验动物使用效率高，重复性好的耐受、急性排斥、慢性排斥肾移植模型，及移植肾功能动态无创检测方法。
方法：封闭群Wistar大鼠用于解剖及预实验。采用改良左侧原位肾移植术，分次顺序使用供体左肾与右肾，动静脉及输尿管端端吻合，7 d后行右侧肾切除。DA、Lewis大鼠分别为供受体作为急性排斥组。F344、Lewis大鼠分别为供受体作为慢性排斥组。Lewis大鼠自体肾移植组作为移植耐受组。观测大鼠双侧肾脏应用解剖，供肾热缺血时间、冷缺血时间，肾移植手术成功率。检测分析肾移植模型生存曲线、蛋白尿水平动态变化。
结果与结论：解剖观测大鼠43例，灌注肾29例，行单侧原位肾移植21例。移植肾热缺血时间小于10 s，冷缺血时间(47.2± 3.7) min，成功率85.7%(18/21)。其中自体肾移植3例，急性排斥肾移植3例，慢性排斥肾移植12例。改良的单侧原位肾移植术简捷经济，分次顺序使用供体左肾与右肾，不干扰受体腹主动脉及下腔静脉血流，重复性好。结果提示，建立的近交系大鼠移植耐受、急性排斥、慢性排斥肾移植模型生存曲线、蛋白尿动态检测具有显著性差异。

关键词: 肾移植, 大鼠, 免疫排斥, 免疫耐受, 肾功能

Abstract:

BACKGROUND: Kidney transplantation in rats is used as an important experimental model for researches of immune tolerance, immune suppression and orange resources.
OBJECTIVE: To develop the kidney transplant models of tolerance, acute and chronic rejection, to investigate surgical technical modifications, and to develop a non-damaging examination of function of the kidney graft.
METHODS: Orthotopic kidney transplantation was performed with end to end anastomosis. The left side kidney of the recipient was replaced by a donor allograft and a contralateral nephrectomy was performed 7 days later. The left and right kidney of the donor could be used apart in-order. Lewis recipients with a DA allograft served as acute rejection group. Lewis recipients with a F344 allograft served as chronic rejection group. Lewis rats of auto-transplantation served as immune tolerance groups. The application anatomies of bilateral kidney, the warm ischemia time and cold ischemia duration of graft, the success ratio of transplantation, as well as the survival curve and proteinuria changes were observed.
RESULTS AND CONCLUSION: A total of 43 rats were anatomized, 29 kidneys were irrigated, and 21 grafts were transplantation. The warm ischemia duration less than 10 seconds, the cold ischemia duration was (47.2±3.7) minutes, and the success ratio of transplantation was 85.7% (18/21), including 3 rats in the autotransplantation group, 3 in the acute rejection group and 12 in the immune tolerance group. The present operation is simplified, stable, and economical. It avoids impairment of the abdomen aorta and the inferior vena cava of recipients．The survival curve and proteinuria trend showed significant differences between acute rejection, chronic rejection and immune tolerance groups.

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