中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (2): 267-271.doi: 10.3969/j.issn.1673-8225.2010.02.018

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

雷公藤、人参与芳维甲酸乙酯对培养光老化真皮成纤维细胞的影响　

钟桂书，何渊民，廖勇梅　   

  1. 泸州医学院附属医院皮肤科，四川省泸州市 646000
  • 出版日期:2010-01-08 发布日期:2010-01-08
  • 作者简介:钟桂书★，女， 1970年生，四川省泸县人，汉族，1995年泸州医学院毕业，硕士，副教授，主要从事光老化疾病和性传播疾病的基础和临床研究。

Effect of tripterygium wilfkrdii Hook, ginseng and arotinoid ethyl ester on the sun-induced aging cultured fibroblasts of the human dermis

Zhong Gui-shu, He Yuan-min, Liao Yong-mei　   

  1. Department of Dermatology, The First Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Online:2010-01-08 Published:2010-01-08
  • About author:Zhong Gui-shu★, Master, Associate professor, Department of Dermatology, The First Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China

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摘要：

背景：基质金属蛋白酶在衰老皮肤真皮成纤维细胞的表达增强，雷公藤红素及人参皂甙具有免疫抑制、抗抗衰老等作用。维甲酸对光老化皮肤有治疗作用，但还存在较多的毒副作用。
目的：对比观察人参皂甙Rd、雷公藤红素与芳维甲酸乙酯对体外培养的人类成纤维细胞基质金属蛋白酶1和基质金属蛋白酶3表达的调节作用。
方法：标本取之于四川省泸州医学院附属医院普外科健康新生儿包皮环切术后真皮，家属知情同意。分离及体外培养人体皮肤成纤维细胞。实验分为①正常对照组：不作任何处理。②阳性对照组：给予紫外线照射(80 kJ/m2)、8-甲氧补骨脂素   (100 mg/L)。③芳维甲酸乙酯实验组：紫外线照射+8-甲氧补骨脂素+芳维甲酸乙酯。④雷公藤红素组：紫外线照射+8-甲氧补骨脂素+雷公藤红素。⑤人参皂甙Rd组：紫外线照射+8-甲氧补骨脂素+人参皂甙Rd。雷公藤红素组：按雷公藤红素剂量分为10，20，40 mg/L3组；人参皂甙Rd组：按人参皂甙Rd剂量分为20，50，100 mg/L 3组。观察各组成纤维细胞基质金属蛋白酶1和基质金属蛋白酶3免疫组织化学染色结果。
结果与结论：阳性对照组基质金属蛋白酶1和基质金属蛋白酶3表达明显增强，与对照组之间差异有显著性(P < 0.05)；芳维甲酸乙酯实验组、雷公藤红素组各组、人参皂甙Rd组各组，与阳性对照组比较，表达明显减弱(P < 0.05)；芳维甲酸乙酯实验组、雷公藤红素组各组、人参皂甙Rd组各组之间比较，差异无显著性(P > 0.05)。结果表明，①人参皂甙Rd、雷公藤红素可以达到芳维甲酸乙酯一样治疗和预防皮肤光老化的作用。②人参、雷公藤等中药的毒副作用小的可能原因是其对机体作用的有效浓度范围较大。③人参皂甙Rd、雷公藤红素和芳维甲酸乙酯治疗和预防皮肤光老化的机制主要是通过调节基质金属蛋白酶1、基质金属蛋白酶3的表达。

关键词: 雷公藤红素, 人参皂甙Rd, 芳维甲酸乙酯, 细胞培养, 光老化, 成纤维细胞, 基质金属蛋白酶, 皮肤组织工程

Abstract:

BACKGROUND: The expression of matrix metalloproteinases (MMPs) is enhanced in fibroblasts of aging skin; tripterine and ginsenoside Rd possess immunosuppression and anti-aging effects. Arotinoid ethyl ester can used to treat sun-induced aging skin, which accompanied by many side effects.
OBJECTIVE: To discuss the regulation role of ginsenoside Rd, celastrol, arotinoid ethyl ester on MMP-1 and MMP-3 of the cultured fibroblasts of the human dermis in vitro.
METHODS: The human dermis circumcision by surgical excision on newborn was obtained from Department of Surgery, Luzhou Medical College, and the informed consent obtained from patients. Culture of fibroblasts were as follow: ①No treatment in the normal control group. ②Radiated with 80 kJ/m2 ultraviolet and treated by 100 mg/L 8-methoxypsoralen in the positive control group. ③Treated by ultraviolet radiation+8-methoxypsoralen+arotinoid ethyl ester in the arotinoid ethyl ester group. ④Treated by ultraviolet radiation +8-methoxypsoralen+triptolide in the triptolide group. ⑤Treated by ultraviolet radiation+8-methoxypsoralen+ginsenoside Rd in the ginsenoside Rd group. The triptolide group was divided into 3 groups with doses of 10, 20, and 40 mg/L. The ginsenoside Rd group was divided into 3 groups with doses of 20, 50 and 100 mg/L. The immunohistochemistry staining of MMP-1 and MMP-3 of the fibroblasts in each group was observed.
RESULTS and CONCLUSION: The expressions of MMP-1and MMP-3 were significantly increased in the positive control group, which had a significant difference to the normal control group (P < 0.05). Compared to the positive control group, the expression of MMP-1and MMP-3 significantly decreased in the treatment groups (P < 0.05); however, the differences among each treatment groups had no significance (P > 0.05). The results demonstrated that: ①Ginsenoside Rd and tripterine exhibit comparably effect on prevent and cure sun-induced aging skin. ②The wide ranges of effective concentration lead to little side effect to the body. ③The action mechanisms of ginsenoside Rd, celastrol and arotinoid ethyl ester in preventing and curing sun-induced aging skin is adjusting the expressions of MMP-1 and MMP-3.

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