中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (20): 3629-3632.doi: 10.3969/j.issn.1673-8225.2010.20.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌激素与辛伐他汀序贯法干预对去势大鼠骨质疏松的治疗作用

马庆芬1，姚珍薇2，单体欣1   

  1. 1山东省寿光市人民医院妇科，山东省寿光市262700；
    2重庆医科大学附属第一医院，重庆市400016
  • 出版日期:2010-05-14 发布日期:2010-05-14
  • 作者简介:马庆芬，女，1979年生，山东省寿光市人，汉族， 2003年重庆医科大学毕业，硕士，医师，主要从事生殖内分泌，绝经后骨质疏松的防治研究。 mqf0408@163.com

Effects of sequential application of estrogen and simvastatin therapy on osteoporosis in ovariectomized rats

Ma Qing-fen1, Yao Zhen-wei2, Shan Ti-xin1   

  1. 1Department of Gynecology, Peoples’ Hospital of Shouguang City, Shouguang  262700, Shandong Province, China;
    2First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Ma Qing-fen, Master, Physician, Department of Gynecology, Peoples’ Hospital of Shouguang City, Shouguang 262700, Shandong Province, China mqf0408@163.com

PDF

442

被引次数

8

摘要：

背景：Frost根据骨重建的概念创建了骨重建干预理论——序贯疗法，即在骨吸收抑制剂之后可给予刺激骨形成的药物。

目的：基于骨重建干预理论，序贯应用雌激素与辛伐他汀干预骨重建吸收期和形成期，观察其对去势大鼠骨质疏松的治疗作用。

方法：3月龄雄性SD大鼠40只，以随机数字表法分为去势组与正常对照组。去势组切除双侧卵巢，正常对照组只进行下腹部皮肤单纯切开术。大鼠去势后1个月，将去势组随机分为3组：序贯组、雌激素组、去势对照组，并开始药物干预：序贯组，皮下注射苯甲酸雌二醇0.1 mg/kg，3 d给药1次，2周后，灌胃给予辛伐他汀5 mg/(kg·d)2周，停药5周，再应用辛伐他汀5 mg/(kg·d)灌胃2周；雌激素组，皮下注射苯甲酸雌二醇0.1 mg/kg，每3 d给药1次，连续用药11周；去势对照组，单纯的饲料喂养，无药物干预。11周后，双能X射线骨密度仪测定股骨骨密度，放射免疫法检测血清白细胞介素6、骨钙素水平。

结果与结论：各治疗组大鼠股骨骨密度、骨钙素水平高于去势对照组(P < 0.05)，并且序贯组明显高于雌激素组(P < 0.05)。各治疗组白细胞介素6水平低于去势对照组(P < 0.05)，并且序贯组低于雌激素组(P < 0.05)。说明雌激素和辛伐他汀序贯疗法可以通过抑制骨吸收，促进骨形成有效地治疗骨质疏松。

关键词: 雌激素, 辛伐他汀, 骨质疏松, 序贯疗法, 骨组织工程

Abstract:

BACKGROUND: Frost founded the theory of bone remodeling intervention, named sequential application, which administrate a promoting bone drug followed by an inhibitor of bone resorption.

OBJECTIVE: According to the bone remodeling intervention theory, to investigate the effects of sequential application of estrogne and simvastatin on osteoporosis in ovariectomized rats.

METHODS: Totally 40 SD male rats with 3-month-old were randomly divided into ovariectomized group and normal control group. In the ovariectomized group, double ovaries of rats were removed. In the normal control group, only the hypogastrium skins of rats were exposed. At 1 month after operation, the ovariectomized group were randomly allocated into sequential estradiol benzoate and simvastatin (sequential group), estrogen group and ovariectomized control group, and received medicine intervention as follows: coherent group: subcutaneous injected estradiol benzoate (0.1 mg/kg) per triduum for 2 weeks, followed by intragastric administrated simvastatin (5 mg/kg per day) for 2 weeks, the medication was removed for 5 weeks, and then intragastric administrated simvastatin (5 mg/kg per day) for 2 weeks; estrogen group: subcutaneous injected estradiol benzoate (0.1 mg/kg) per triduum for 11 weeks; in the ovariectomized control group, rats were given animal feeds only. After 11 weeks, the bone mineral density (BMD) was measured by double X-ray densitometry, and interleukin-6 (IL-6) and osteocalcin levels were detected by radioimmunoassay.

RESULTS AND CONCLUSION: The BMD and osteocalcin levels were greater in treated groups than the ovariectomized control group (P < 0.05); especially higher in the sequential group than the estrogen group (P < 0.05). Meantime, the expression of IL-6 in the treated groups were degraded compared to the ovariectomized control group (P < 0.05), which was lower in the sequential group than the estrogen group (P < 0.05). The results demonstrated that sequential application of estrogen and simvastatin can prevent osteoporosis by inhibiting bone resorption and improving bone formation.

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