中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (27): 4314-4319.doi: 10.12307/2022.861

• 组织工程骨材料Tissue-engineered bone • 上一篇    下一篇

聚己二酸丁二醇酯-对苯二甲酸丁二醇酯/Ⅰ型胶原取向纤维促进前交叉韧带断裂后腱-骨愈合

胡秋羽1，杨  龙2，杨  勇2，宋沈超1   

  1. 1贵州医科大学公共卫生学院，环境污染与疾病监控教育部重点实验室，贵州省贵阳市  550025；2贵州医科大学附属医院骨科，贵州省贵阳市  550004
  • 收稿日期:2021-03-03 接受日期:2021-04-10 出版日期:2022-09-28 发布日期:2022-03-10
  • 通讯作者: 宋沈超，教授，贵州医科大学公共卫生学院，环境污染与疾病监控教育部重点实验室，贵州省贵阳市 550025
  • 作者简介:胡秋羽，女，1989年生，贵州省贵阳市人，汉族，贵州医科大学在读硕士，主要从事组织工程研究。 杨龙，男，1988年生，贵州省贵阳市人，汉族，贵州医科大学在博士，主治医师，主要从事运动医学研究。
  • 基金资助:
    国家自然科学基金资助项目(81802175)，项目负责人：杨龙；贵州省科技厅资助项目(黔科合基础[2020]1Y312)，项目负责人：杨龙；贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85号)，项目负责人：宋沈超

Aligned poly(butylene adipate-co-terephthalate)/type I collagen fibers promote tendon-bone healing after anterior cruciate ligament rupture

Hu Qiuyu1, Yang Long2, Yang Yong2, Song Shenchao1   

  1. 1School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou Province, China; 2Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Received:2021-03-03 Accepted:2021-04-10 Online:2022-09-28 Published:2022-03-10
  • Contact: Song Shenchao, Professor, School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou Province, China
  • About author:Hu Qiuyu, Master candidate, School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou Province, China Yang Long, Doctoral candidate, Attending physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China Hu Qiuyu and Yang Long contributed equally to this article.
  • Supported by:
    the National Natural Science Foundation of China, No. 81802175, (to YL); a grant from Department of Science and Technology of Guizhou Province, No. [2020]1Y312, (to YL); the First-Class Discipline Construction Project in Guizhou Province - Public Health and Preventive Medicine, No. 2017[85] (to SSC)

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摘要：

文题释义：
聚己二酸丁二醇酯-对苯二甲酸丁二醇酯：是一种通过己二酸聚酯和对苯二甲酸二甲酯之间的酯交换反应合成的无规共聚物，具有良好的力学性能和生物相容性，以及一系列多变的特性，如生物降解性和高断裂伸长率，但生物活性较低。
腱-骨愈合：指肌腱与骨之间的生长愈合，以及相应的组织改变过程，其特征性结构为移行的纤维软骨带；腱-骨愈合方式大体上可分为直接愈合和间接愈合。

背景：材料表面的微/纳米结构对细胞的行为具有调控作用，肌腱组织主要由平行的胶原纤维组成，因此取向纤维结构对腱-骨愈合具有一定的促进作用。
目的：探索聚己二酸丁二醇酯-对苯二甲酸丁二醇酯[poly(butylene adipate-co-terephthalate)，PBAT]/Ⅰ型胶原取向纤维支架的生物相容性和成骨诱导活性。
方法：采用静电纺丝技术分别制备无序PBAT、取向PBAT和取向PBAT/Ⅰ型胶原纤维支架材料，表征3种支架的静接触角与力学性能。将3种支架分别与大鼠骨髓间充质干细胞共培养，检测细胞黏附率与增殖情况，DAPI染色和Live/Dead 荧光染色观察细胞在支架上的黏附和存活情况，碱性磷酸酶染色和Ⅰ型胶原免疫组化染色检测取向纤维支架的成骨诱导活性。
结果与结论：①取向PBAT/Ⅰ型胶原纤维支架的静接触角低于无序PBAT、取向PBAT纤维支架(P < 0.05)；②两种取向支架的应变程度优于无序PBAT支架(P < 0.05)，取向PBAT支架的抗拉强度优于无序PBAT组(P < 0.05)，3组支架的弹性模量比较差异无显著性意义(P > 0.05)；③取向PBAT/Ⅰ型胶原纤维支架上的细胞黏附与增殖情况优于其他两组支架；④DAPI染色和Live/Dead 荧光染色显示，骨髓间充质干细胞均可在3组支架上黏附和增殖，其中以取向PBAT/Ⅰ型胶原纤维支架上的细胞存活数量最多；⑤碱性磷酸酶染色和Ⅰ型胶原免疫组化染色显示，取向PBAT/Ⅰ型胶原纤维支架的成骨诱导活性强于取向PBAT纤维支架；⑥结果表明，PBAT/Ⅰ型胶原取向纤维支架具有良好的生物相容性和成骨诱导活性，能有效促进细胞增殖、黏附。
缩略语：聚己二酸丁二醇酯-对苯二甲酸丁二醇酯：poly (butylene adipate-co-terephthalate)，PBAT

https://orcid.org/0000-0001-7800-8836 (胡秋羽) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 聚己二酸丁二醇酯-对苯二甲酸丁二醇酯, Ⅰ型胶原, 组织工程, 取向纤维, 干细胞, 静电纺丝, 生物材料

Abstract: BACKGROUND: The micro/nano structure on the surface of the material has a regulatory effect on the behavior of the cells. The tendon tissue is mainly composed of parallel collagen fibers, so the aligned fiber structure has a certain promotion effect on tendon-bone healing.
OBJECTIVE: To explore the biocompatibility and osteoinductive activity of poly(butylene adipate-co-terephthalate) (PBAT)/type I collagen aligned fiber scaffolds.
METHODS: Electrospinning technology was used to prepare random PBAT, aligned PBAT, and aligned PBAT/type I collagen fiber scaffold materials. The static contact angle and mechanical properties of the three stents were characterized. The three scaffolds were co-cultured with rat bone marrow mesenchymal stem cells to detect cell adhesion rate and proliferation. DAPI staining and Live/Dead fluorescent staining were used to observe cell adhesion and survival on the scaffold. Alkaline phosphatase staining and type I collagen immunohistochemical staining were used to detect the osteoinductive activity of oriented fiber scaffolds.
RESULTS AND CONCLUSION: (1) The static contact angle of oriented PBAT/type I collagen fiber scaffold was lower than that of random PBAT and aligned PBAT fiber scaffolds (P < 0.05). (2) The strain degree of the two aligned scaffolds was better than that of the aligned PBAT scaffold (P < 0.05), and the tensile strength of the aligned PBAT scaffold was better than that of the random PBAT group (P < 0.05). There was no significant difference in the elastic modulus of the three groups of scaffolds (P > 0.05). (3) The cell adhesion and proliferation on the aligned PBAT/type I collagen fiber scaffold were better than those of the other two scaffolds. (4) DAPI staining and Live/Dead fluorescent staining showed that bone marrow mesenchymal stem cells could adhere and proliferate on the three groups of scaffolds, and the number of cells on the aligned PBAT/type I collagen fiber scaffold was the largest. (5) Alkaline phosphatase staining and type I collagen immunohistochemical staining showed that the osteoinductive activity of the aligned PBAT/type I collagen fiber scaffold was stronger than that of the aligned PBAT fiber scaffold. (6) The results show that the PBAT/type I collagen aligned fiber scaffold has good biocompatibility and osteoinductive activity and can effectively promote cell proliferation and adhesion.

Key words: poly(butylene adipate-co-terephthalate), type I collagen, tissue engineering, aligned fibers, stem cells, electrospinning, biomaterials

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