中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6147-6151.doi: 10.3969/j.issn.2095-4344.2012.33.014

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

碱性成纤维细胞生长因子基因转染对人牙周韧带细胞的作用

李立恒1，高燕飞2   

  1. 1河北北方学院 附属第一医院，河北省张家口市 075000；
    2河北北方学院、张家口教育学院，河北省张家口市 075000
  • 收稿日期:2012-04-09 修回日期:2012-06-07 出版日期:2012-08-12 发布日期:2012-08-12
  • 通讯作者: 高燕飞，助教，河北北方学院、张家口教育学院，河北省张家口市 075000
  • 作者简介:李立恒，男，1982年生，河北省张家口市人，汉族，2006年河北北方学院口腔医学毕业，助教，主要从事口腔医学研究。 1781839357@qq.com

Effect of basic fibroblast growth factor gene transfection on the proliferation of human periodontal ligament cells

Li Li-heng1, Gao Yan-fei2   

  1. 1First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China;
    2Zhangjiakou Institute of Education, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2012-04-09 Revised:2012-06-07 Online:2012-08-12 Published:2012-08-12
  • Contact: Gao Yan-fei Assistant teacher, Zhangjiakou Institute of Education, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • About author:Li Li-heng, Assistant teacher, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China 1781839357@qq.com

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290

被引次数

2

摘要：

背景：主要来源于因正畸或阻生拔除的健康牙培养而成的人牙周韧带细胞已成为牙周组织工程理想的种子细胞来源之一。
目的：了解采用重组腺相关病毒作为载体介导碱性成纤维细胞生长因子基因转染对体外培养的人牙周韧带细胞增殖及细胞周期的影响。
方法：体外培养人牙周韧带细胞, 经重组腺相关病毒作为载体介导碱性成纤维细胞生长因子基因转染，分为3组：对照组、空载病毒组、碱性成纤维细胞生长因子转染组。用RT-PCR，Western blot检测人牙周韧带细胞在转染前后碱性成纤维细胞生长因子基因和蛋白的表达。应用细胞生长曲线、四甲基偶氮唑蓝比色法观察细胞生长的优化作用；采用流式细胞术测定细胞周期分布的变化。
结果与结论：对照组、空载病毒组未检测到碱性成纤维细胞生长因子mRNA和蛋白表达；碱性成纤维细胞生长因子转染组碱性成纤维细胞生长因子mRNA和蛋白水平均有表达，细胞的生长速度明显增快，细胞周期G0/G1期减少，S期细胞数增多。各组间比较差异有显著性意义(P < 0.05)。

关键词: 碱性成纤维细胞生长因子, 基因转染, 人牙周韧带细胞, 细胞周期, 重组腺相关病毒

Abstract:

BACKGROUND: Human periodontal ligament cells are one of the ideal seed cells in periodontal tissue engineering.
OBJECTIVE: To investigate the effect of basic fibroblast growth factor gene transfection with recombinant adeno-associated virus as a vector on the proliferation and cell cycle of cultured human periodontal ligament cells in vitro.
METHODS: Human periodontal ligament cells cultured in vitro were transfected with basic fibroblast growth factor gene mediated by recombinant adeno-associated virus, and then the transfected cells were divided into three groups: control group, recombinant adeno-associated virus group and basic fibroblast growth factor gene transfection group. Reverse transcription-PCR and western blot were used to assess the gene and protein expression of basic fibroblast growth factor before and after transfection. Cellular proliferation was determined by the cell growth curve and MTT assay. The cell cycle was analyzed by flow cytometry.
RESULTS AND CONCLUSION: There was no basic fibroblast growth factor expression in the control and recombinant adeno-associated virus groups. After transfection, the expression of basic fibroblast growth factor mRNA and protein was found in the basic fibroblast growth factor gene transfection group, the cell growth was accelerated, the cell cycle of G0/G1 phase was decreased and cell number in S phase was increased. There was a significant difference among the groups (P < 0.05).

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