中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (30): 4860-4866.doi: 10.3969/j.issn.2095-4344.2017.30.018

• 药物控释材料 drug delivery materials • 上一篇    下一篇

肠外瘘封堵可降解抗菌水凝胶的制备及体外性能

牛宇翔,熊党生
  

  1. 南京理工大学材料科学与工程学院,江苏省南京市  210094
  • 收稿日期:2017-05-07 出版日期:2017-10-28 发布日期:2017-11-07
  • 通讯作者: 熊党生,硕士,教授,南京理工大学材料科学与工程学院,江苏省南京市 210094
  • 作者简介:牛宇翔,男,1990年生,江苏省南京市人,汉族,2017年南京理工大学材料科学与工程学院毕业,硕士,主要从事生物材料研究。
  • 基金资助:
    国家自然科学基金(51575278);江苏省科技计划项目(BY2016004-08;BA2015054)

Preparation and in vitro properties of degradable antibacterial composite hydrogel for intestinal fistulas

Niu Yu-xiang, Xiong Dang-sheng
  

  1. College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China
  • Received:2017-05-07 Online:2017-10-28 Published:2017-11-07
  • Contact: Xiong Dang-sheng, Master, Professor, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China
  • About author:Niu Yu-xiang, Master, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 51575278; the Science and Technology Plan Project of Jiangsu Province, No. BY2016004-08, BA2015054

摘要:

文章快速阅读:

 

文题释义:
席夫碱反应:通常是指胺和活性羰基缩合反应。实验通过丁二酸酐对壳聚糖进行改性,提高了壳聚糖在中性溶液中的溶解度;采用高碘酸钠对海藻酸钠进行氧化,得到活性羰基。水溶性壳聚糖的氨基和氧化海藻酸钠的羰基可以通过席夫碱反应在5 min内快速混合形成凝胶,且2种组分比例相等时,凝胶时间最快,约2 min。快速的凝胶时间对于外科微创手术具有很高的应用价值。
降解性能:组织工程材料的降解性能是其一项重要指标。对于肠外瘘封堵凝胶而言,可能接触到的体内环境除了生理组织体液外,还有可能接触到部分肠液。实验分别采用磷酸盐缓冲溶液模拟组织体液,胰蛋白酶配置模拟肠液,用于对制备出的凝胶进行为期28 d的体外降解速率研究。胰蛋白酶对壳聚糖的特殊降解作用导致了凝胶材料在模拟肠液中的降解速率要高于在磷酸盐缓冲溶液中的降解速率,28 d后磷酸盐缓冲溶液中的凝胶残余量约为60%,而模拟肠液中的残余量均不超过20%。
 
背景:目前对于肠外瘘的外科手术治疗手段有待改进,寻求一种人工合成水凝胶代替纤维蛋白凝胶作为封堵材料势在必行。
目的:制备出可注射可降解抗菌水凝胶材料,并检测其体外生物学性能。
方法:以水溶性壳聚糖(S-Cts)和氧化海藻酸钠(O-Alg)为原料,采用席夫碱反应制备原位成型凝胶,在此基础上加入氧化还原反应得到的纳米银(nano-Ag)颗粒,形成S-Cts/O-Alg/nano-Ag复合水凝胶。观测凝胶时间和微观形貌,通过模拟生理环境溶液,检测复合水凝胶的溶胀性能、降解性能以及抗菌性能。
结果与结论:①水溶性壳聚糖与氧化海藻酸钠的组分含量越接近,凝胶时间越短,并可以控制在外科手术所需范围内;②2种组分含量的变化会影响形成凝胶的微观结构形貌,水溶性壳聚糖组分含量越大,凝胶网络结构越致密;③复合凝胶具备优秀的溶胀性能,在含有胰蛋白酶的模拟肠液中相比在磷酸盐缓冲溶液中降解速率更快;④凝胶组分中加入纳米银微粒,有效提升了凝胶的抗菌性能;⑤这种材料弥补了单纯天然高分子材料的生物相容性、生物可降解性以及在抑菌能力上的不足,具备一定研究价值和应用前景。

关键词: 生物材料, 材料相容性, 海藻酸钠, 壳聚糖, 可注射水凝胶, 纳米银颗粒, 体外降解, 抗菌, 国家自然科学基金

Abstract:

BACKGROUND: As the surgical treatment for intestinal fistulas nowadays needs to be improved, we are seeking a new kind of artificially synthesized hydrogel to replace fibrin gels as the sealing gel, which is imperative for both economic and social benefits.
OBJECTIVE: To prepare a degradable antibacterial composite hydrogel and to detect the in vitro biological properties.
METHODS: In this study, we combined soluble chitosan (S-Cts) with oxidized alginate (O-Alg) to prepare the injectable and degradable hydrogel under Schiff base reaction. Besides, nanosilver (nano-Ag) particles were added to obtain S-Cts/O-Alg/nano-Ag composite hydrogel. Gelation time, microstructure, swelling, degradation, and antibacterial properties of the composite hydrogel were observed and detected in simulated physiological environment.
RESULTS AND CONCLUSION: The closer constituent contents of water-soluble chitosan and sodium alginate indicate the shorter gelation time, and the time could be controlled within the range of surgery. The variation in the constituent content of the two components can affect the hydrogel microstructure. The higher constituent content of water-soluble chitosan implicates the denser network of the hydrogel. The composite hydrogel has excellent swelling properties, and it degrades faster in the simulated intestinal fluid containing trypsin than in the PBS. Moreover, adding nanosilver particles can bring certain antibacterial properties. This hydrogel has better biocompatibility, biodegradability and antibacterial ability than natural macromolecules, and has certain research value and application prospect.

Key words: Alginates, Chitosan, Silver, Hydrogel, Tissue Engineering

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