中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (4): 559-563.doi: 10.3969/j.issn.2095-4344.2017.04.011

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

正畸牙移动中热休克蛋白70在牙周组织中的表达

聂 晶1,姜 涵2,李泽慧2,潘 旭1,李春霞1   

  1. 新疆医科大学第一附属医院,1口腔正畸科,2牙周粘膜科,新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2017-01-06 出版日期:2017-02-08 发布日期:2017-03-13
  • 通讯作者: 通讯作者:聂晶,新疆医科大学第一附属医院口腔正畸科,新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:聂晶,男,1980年生,河南省扶沟县人,汉族,硕士,主要从事错合畸形防治的研究。
  • 基金资助:

    新疆维吾尔自治区自然科学基金青年项目(2014211C080)

Expression of heat shock protein 70 in periodontium during orthodontic tooth movement

Nie Jing1, Jiang Han2, Li Ze-hui2, Pan Xu1, Li Chun-xia1   

  1. 1Department of Orthodontics; 2Department of Periodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2017-01-06 Online:2017-02-08 Published:2017-03-13
  • Contact: Corresponding author: Nie Jing, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Nie Jing, Master, Department of Orthodontics, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the Youth Project of the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2014211C080

摘要:

文章快速阅读:

文题释义:
热休克蛋白:又称应激蛋白,是机体细胞在一些理化因素刺激后高效表达的一组蛋白质,因最先发现于果蝇唾液腺的热应激反应中而得名,广泛存在于人、动物、微生物和植物的细胞中,是一类在遗传上高度保守的分子,能保护细胞并促进细胞对各种刺激所造成的损伤进行自身修复,具有重要的生物学功能。
正畸治疗:主要通过各种矫正装置来调整面部骨骼、牙齿及颌面部的神经及肌肉之间的协调性,也就是调整上下颌骨之间,上下牙齿之间、牙齿与颌骨之间和联系它们的神经及肌肉之间不正常的关系,其最终矫治目标是达到口颌系统的平衡、稳定和美观。
摘要
背景:
学者们对正畸力作用下牙周组织的改建做了大量的研究,但对于在大鼠牙齿移动过程中热休克蛋白70在牙周组织中表达的研究尚未涉及。
目的:观察在正畸牙移动过程中大鼠牙周组织热休克蛋白70的表达。
方法:采用NiTi拉簧建立大鼠正畸牙移动模型,力值50 g。分别于加力后1,3,5,7,10,14 d检测正畸牙周组织热休克蛋白70的表达。
结果与结论:①免疫荧光染色显示,正畸加力1 d,牙周组织中热休克蛋白70的表达量增强,至加力后5 d时表达量最大,之后逐渐降低,第14天时回落至初始水平;②结果说明,正畸力可以刺激牙周组织中热休克蛋白70的表达,热休克蛋白70可能参与了牙周组织的改建。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0001-8173-3430(聂晶)

关键词: 组织构建, 组织工程, 热休克蛋白, 牙周组织, 正畸, 牙移动, 大鼠, 免疫荧光, 组织改建, 成骨细胞, 破骨细胞, 新疆维吾尔自治区自然科学基金

Abstract:

Abstract
BACKGROUND:
Extensive research has been concentrated on the reconstruction of periodontium under orthodontic force. However, there are no reports regarding the expression of heat shock protein 70 (HSP70) in rat periodontium during orthodontic tooth movement.
OBJECTIVE: To investigate the expression of HSP70 in rat periodontium during orthodontic tooth movement.
METHODS: Rat orthodontic tooth movement models were established using NiTi extension spring with the force of 50 g. The orthodontic periodontium was collected at 1, 3, 5, 7, 10 and 14 days after exerting force to detect the expression of HSP70. 
RESULTS AND CONCLUSION: According to immunofluorescence staining, the expression of HSP70 in the periodontal tissues was increased on day 1 after exerting force, and reached the highest level on day 5, then decreased to the initial level on day 14. These results suggest that the expression of HSP70 is evoked by orthodontic force, and it may play a role in periodontal remodeling.

Key words: Tissue Engineering, Heat-Shock Proteins, Osteoblasts

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