中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (51): 7717-7723.doi: 10.3969/j.issn.2095-4344.2016.51.018

• 组织构建综述 tissue construction review • 上一篇    下一篇

骨髓间充质干细胞向软骨及骨分化:Wnt5a/PCP信号通路作用的研究与进展

彭  旭1,张晓梅1,魏诗航2,刘  艳1,何学令1   

  1. 四川大学,1实验动物中心,2生命科学院,四川省成都市  610041
  • 收稿日期:2016-09-10 出版日期:2016-12-09 发布日期:2016-12-09
  • 通讯作者: 通讯作者:何学令,博士,副研究员,四川大学实验动物中心,四川省成都市 610041 通讯作者:刘艳,硕士,助理研究员,四川大学实验动物中心,四川省成都市 610041
  • 作者简介:彭旭,男,四川省绵竹市人,汉族,硕士,助理实验师,主要从事动物实验、细胞生物学实验工作。
  • 基金资助:

    国家自然科学基金(51573112);四川省科技厅科技支撑项目(2015SZ0075)

Bone marrow mesenchymal stem cells differentiate into cartilage and bone: roles of Wnt5a/PCP signaling pathways

Peng Xu1, Zhang Xiao-mei1, Wei Shi-hang2, Liu Yan1, He Xue-ling1   

  1. 1Laboratory Animal Center, Sichuan University, Chengdu 610041, Sichuan Province, China; 2College of Life Science, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2016-09-10 Online:2016-12-09 Published:2016-12-09
  • Contact: Corresponding author: He Xue-ling, M.D., Associate researcher, Laboratory Animal Center, Sichuan University, Chengdu 610041, Sichuan Province, China Corresponding author Liu Yan, Master, Assistant researcher, Laboratory Animal Center, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Peng Xu, Master, Assistant experimentalist, Laboratory Animal Center, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 51573112; the Science and Technology Project of Science & Technology Department of Sichuan Province, No. 2015SZ0075

摘要:

文章快速阅读:

文题释义:
Wnt5a:是Wnt家族中的重要成员之一,主要介导G蛋白激活非经典Wnt/Ca2+信号通路,特异的Wnt配体Frizzled2主要通过G蛋白导致细胞内钙的释放,再传递信号激活下游靶基因,参与细胞运动、细胞增殖和细胞极性。
PCP信号通路:是一类通过小G蛋白(Rho或者Rac)介导的actin动态变化(聚合与解聚)调节细胞极性和细胞运动的通路,属于非经典Wnt通路。PCP信号通路由3类分子组成:PCP上游分子(Wnt5a,Wnt5b,Wnt11),PCP核心分子(Fzd,Flamingo,Diego等)和PCP下游效应分子(RhoA、JNK、NLK等)。

摘要
背景
:Wnt5a具有抑制经典Wnt信号和激活非经典Wnt信号途径的生物学功能。近年来,研究发现Wnt5a介导的Wnt5a/PCP非经典信号通路在骨髓间充质干细胞的增殖和分化过程中发挥着重要作用。但其参与骨髓间充质干细胞向软骨和骨分化形成的机制并不完全清楚。
目的:综述Wnt5a/PCP信号通路中的相关下游效应分子,及其在骨髓间充质干细胞向软骨和骨分化形成过程中作用的最新研究进展。
方法:以“间充质干细胞、Wnt信号通路、软骨分化、骨分化”为中文捡索词,以“BMSCs、Chondrogenic differentiation、Osteogenic differentiation、Wnt、Fzd、Ror2、RhoA、ROCK、JNK”为英文检索词,在维普、中国知网(CNKI)期刊全文数据库和PubMed 数据库检索2000年1月至2016年2月有关Wnt信号通路参与间充质干细胞向软骨及骨分化的文献。排除陈旧性和重复性研究,重点分析43 篇文献进行综述。
结果与结论:Wnt5a是非经典Wnt信号途径的代表性Wnt蛋白,主要通过其下游信号分子Fzd、Ror、RhoA、ROCK、JNK等介导,参与细胞骨架、细胞迁移和细胞极化等活动,从而调控其增殖和分化。然而Wnt5a/PCP信号通路在骨髓间充质干细胞向软骨和骨分化过程中具体的作用机制并不清楚,下游效应分子之间是如何协同或独立发挥作用也是未知的,这些问题均需要进一步深入研究。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-0094-7099(何学令)

关键词: 组织构建, 骨组织工程, 骨髓间充质干细胞, Wnt5a/PCP信号通路, 软骨分化, 软骨极性, 骨形成, Fzd, Ror2, RhoA, ROCK, JNK, 国家自然科学基金

Abstract:

BACKGROUND: Wnt5a is able to inhibit canonical Wnt signaling and activate non-canonical Wnt signaling pathway. In recent years, it has been found that non-classical Wnt5a/PCP signaling pathway mediated by Wnt5a plays an important role in the process of bone marrow mesenchymal stem cell proliferation and differentiation, but the underlying mechanism is unclear.
OBJECTIVE: To summarize the progress in downstream effector molecules related to Wnt5a/PCP signaling pathway, and its roles in the chondrogenic and osteogenic differentiation of bone marrow mesenchymal stem cells.
METHODS: A computer-based online search of CqVip, CNKI and PubMed databases between January 2000 and February 2016 was performed using the Chinese keywords of “BMSCs, Wnt signaling pathways, chondrogenic differentiation, osteogenic differentiation” and English keywords of “BMSCs, chondrogenic differentiation, osteogenic differentiation, Wnt, Fzd, Ror2, RhoA, ROCK, JNK”, respectively. Literatures related to bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation were selected. Finally, 43 eligible articles were included for analysis through excluding the old and repeated research.
RESULTS AND CONCLUSION: Wnt5a, a representative protein in non-canonical Wnt signaling pathway, paticipates in the cytoskeleton, cell migration and cell polarization and other activities by mediating its downstream signaling molecules such as Fzd, Ror, RhoA, ROCK, JNK, thereby regulating its proliferation and differentiation. But it is unclear how Wnt5a/PCP participates in the bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation and how the downstream effector molecules interact or function independently, which requires further studies.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Mesenchymal Stem Cells, Cartilage, Osteogenesis, Tissue Engineering

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