中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (25): 3680-3686.doi: 10.3969/j.issn.2095-4344.2016.25.005

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

转化生长因子β1对多孔钽/MG63成骨样细胞复合物细胞增殖及分泌功能的影响

庞海涛1，甘洪全2，王 茜3，崔逸爽4，赖振权4，周国龙4，潘祥宇4，王志强2，李琪佳4   

  1. 1华北理工大学附属骨科医院手科(唐山第二医院)，河北省唐山市 063000；2华北理工大学附属医院骨科，河北省唐山市 063000；华北理工大学，3基础医学院，4医学实验中心，河北省唐山市 063000
  • 收稿日期:2016-04-14 出版日期:2016-06-17 发布日期:2016-06-17
  • 通讯作者: 李琪佳，教授，硕士生导师，华北理工大学医学实验中心，河北省唐山市 063000
  • 作者简介:庞海涛，男，1979年生，汉族，河北省唐山市人，2009年华北理工大学毕业，硕士，主治医师，主要从事骨组织工程研究。
  • 基金资助:

    国家科技部科技支撑课题(2012BAE06B03)；河北省科技支撑课题(16277776D)；河北省医学科学研究重点课题计划资助项目(20160225)；华北理工大学大学博士科研启动基金资助项目

 Effect of transforming growth factor beta1 on proliferation and secretion of osteoblasts on porous tantalum/MG63 osteoblast-like cell composites

Pang Hai-tao1, Gan Hong-quan2, Wang Qian3, Cui Yi-shuang4, Lai Zhen-quan4, Zhou Guo-long4,      Pan Xiang-yu4, Wang Zhi-qiang2, Li Qi-jia4   

  1. 1Department of Hand Surgery, Affiliated Orthopedics Hospital of North China University of Science and Technology (Tangshan second Hospital), Tangshan 063000, Hebei Province, China; 2Department of Orthopedics, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 3Basic Medical School, 4Medical Experimental Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Received:2016-04-14 Online:2016-06-17 Published:2016-06-17
  • Contact: Li Qi-jia, Professor, Master’s supervisor, Medical Experimental Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Pang Hai-tao, Master, Attending physician, Department of Hand Surgery, Affiliated Orthopedics Hospital of North China University of Science and Technology (Tangshan second Hospital), Tangshan 063000, Hebei Province, China
  • Supported by:

    the Project Supported by State Commission of Science Technology of China, No. 2012BAE06B03; the Science and Technology Project of Hebei Province, No. 16277776D; the Medical Research Key Planning Project of Hebei Province, No. 20160225; Supported by Research Fund for the Doctoral Program of North China University of Science and Technology

PDF

458

摘要：

文章快速阅读：

 

 

文题释义：

 

转化生长因子β：是一组多功能蛋白质，属于转化生长因子超家族成员。可以影响多种细胞的生长，分化、细胞凋亡及免疫调节等功能。转化生长因子β包括3个亚型，转化生长因子β1、转化生长因子β2和转化生长因子β3，3种亚型功能相似，一般来说，对间充质起源的细胞起刺激作用，而对上皮或神经外胚层来源的细胞起抑制作用。

 

多孔钽材料：有着与其他金属材料不同的独特性质，即三维立体空间构型，良好的力学性能，其弹性模量介于皮质骨和松质骨之间，在体内有较小的应力遮挡，利于骨传导及骨骼重塑。

 
 

摘要

 

背景：前期研究已得出国产多孔钽无毒性，具有良好的生物相容性，具有促成骨作用。

 

目的：观察转化生长因子β1对国产多孔钽/MG63成骨样细胞复合物中成骨细胞生长增殖、细胞周期及分泌功能的影响，

 

方法：取生长状态良好的第3代MG63成骨样细胞悬液，按1×109 L-1的细胞浓度接种于多孔钽材料上，分别以含0，0.5，5，10 µg/L转化生长因子β1的培养基培养。CCK-8法检测培养1-13 d成骨细胞增殖水平；培养5，9 d，扫描电镜及透射电镜观察培养细胞形态及超微结构，检测细胞分泌Ⅰ型胶原水平。

 

结果与结论：①0.5，5，10 µg/L转化生长因子β1均可促进多孔钽上成骨细胞的增殖，其中以5 µg/L转化生长因子β1的促增殖作用最明显；②在5 µg/L转化生长因子β1作用下，成骨细胞在多孔钽支架表面及微孔内形成叠层黏附生长，向材料表面伸出较多的伪足，细胞分泌的基质基本覆盖多孔钽表面，胞浆内可见大量粗面内质网、游离核糖体及致密分布的线粒体，高尔基复合体较发达，并且胞质内出现较多基质小泡；③5 µg/L转化生长因子β1组培养5，9 d的Ⅰ型胶原水平高于0.5及10 µg/L转化生长因子β1组(P < 0.05)；④结果表明：转化生长因子β1可促进国产多孔钽表面MG63成骨样细胞的增殖，其中以5 µg/L转化生长因子β1对成骨细胞的促增殖及Ⅰ型胶原分泌作用最明显。

 

 

 

ORCID: 0000-0003-4093-9058(李琪佳)

关键词: 生物材料, 骨生物材料, 多孔钽, 转化生长因子β1/多孔钽/MG63成骨样细胞, Ⅰ型胶原, 成骨细胞

Abstract:

BACKGROUND: Previous studies have demonstrated that the Chinese porous tantalum made in China has non-toxicity and good biocompatibility, which can promote osteogenesis.        

OBJECTIVE: To investigate the effects of transforming growth factor β1 on proliferation, cell cycle and secretion of osteoblasts on porous tantalum/MG63 osteoblast-like cell composites.
METHODS: Passage 3 MG63 osteoblast-like cell suspension (1×109/L) was seeded onto the porous tantalum, then the cell composites were inoculated in the medium with 0, 0.5, 5 and 10 µg/L transforming growth factor β1, respectively. The proliferation of osteoblasts was detected by cell counting kit-8 assay at 1-13 days after inoculation; the cell morphology and ultrastructure observed by scanning electron microscope and transmission electron microscopy; and level of collagen type I detected by enzyme-linked immunosorbent assay.

RESULTS AND CONCLUSON: 0.5, 5, 10 µg/L transforming growth factor β1 could promote the osteoblast proliferation, and cell proliferation in the 5 µg/L transforming growth factor β1 group was higher than that in the other groups; in the 5 µg/L transforming growth factor β1 group, laminated osteoblasts adhered on the surface and grew into inner of porous tantalum, which extended more pseudopodia toward the scaffold; osteoblasts-secreted matrix could cover the scaffold and numerous rough endoplasmic reticulum, free ribosomes, dense mitochondria, Golgi apparatus as well as matrix vesicles could be found in the cytoplasm. In addition, the level of collagen type I in the 5 µg/L transforming growth factor β1 group was significantly higher than that in the other groups (P < 0.05). These results indicate that transforming growth factor β1 can promote proliferation, and collagen type I secretion of osteoblasts on porous tantalum/MG63 osteoblast-like cell composites, and the optimum mass concentration of transforming growth factor β1 is 5 µg/L.

 

Key words: Osteoblasts, Tantalum, Transforming Growth Factor beta1, Tissue Engineering

中图分类号: