中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (4): 882-891.doi: 10.12307/2026.550

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

心俞穴位埋线预处理改善急性心肌缺血模型大鼠的心功能

陈小青1，2，卞路瑶1，陆星宇1，杨  涛1，李湘海3   

  1. 1贵州医科大学，贵州省贵阳市  550004；2隆昌市人民医院，四川省内江市  642150；3贵州医科大学附属医院中医科，贵州省贵阳市  550004
  • 收稿日期:2024-10-12 接受日期:2024-12-06 出版日期:2026-02-08 发布日期:2025-05-19
  • 通讯作者: 李湘海，博士，主任医师，贵州医科大学附属医院中医科，贵州省贵阳市 550004
  • 作者简介:陈小青，男，1992年生，四川省内江市人，汉族，贵州医科大学在读硕士，主要从事中医药对心脑血管疾病的防治研究。
  • 基金资助:
    贵州省卫生健康委科学技术基金项目(gzwkj2024-291)，项目负责人：李湘海；贵州医科大学附属医院国家自然科学基金面上项目培育计划项目(gyfynsfc[2023]-56)，项目负责人：李湘海；贵州医科大学教学改革研究重点项目(JG2021012)，项目负责人：李湘海

Thread embedding pretreatment at Xinshu (BL 15) improves cardiac function of acute myocardial ischemia rats

Chen Xiaoqing1, 2, Bian Luyao1, Lu Xingyu1, Yang Tao1, Li Xiang Hai3   

  1. 1Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2Longchang People’s Hospital, Neijiang 642150, Sichuan Province, China; 3Department of Traditional Chinese Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Received:2024-10-12 Accepted:2024-12-06 Online:2026-02-08 Published:2025-05-19
  • Contact: Li Xianghai, PhD, Chief physician, Department of Traditional Chinese Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Chen Xiaoqing, Master candidate, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Longchang People’s Hospital, Neijiang 642150, Sichuan Province, China
  • Supported by:
    Science and Technology Fund Project of Guizhou Provincial Health Commission, No. gzwkj2024-291 (to LXH); National Natural Science Foundation General Project Cultivation Program for Guizhou Medical University Affiliated Hospital, No. gyfynsfc[2023]-56 (to LXH); Key Project of Teaching Reform Research at Guizhou Medical University, No. JG2021012 (to LXH)

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摘要：




文题释义：
心俞：心俞穴首见于《灵枢·背腧》，属于足太阳膀胱经，是心脏之气输注背部的穴位，具有宁心安神、理气调血的作用。据穴位解剖资料，《图注八十一难辨真》曰：“心病及气血，当以俞穴为治”。大量临床和实验研究表明，电针心俞穴可显著改善急性心肌缺血心功能，对缺血性心脏病的心肌细胞亦有保护作用。
核因子κB：是一种多效性基因转录因子，一般情况下核因子κB以非活性形式保留在细胞质中。核因子κB激活常伴有P65亚型的核内易位，P65水平的升高是核因子κB信号通路活化的标志，激活后参与许多基因的转录调控，在免疫、炎症、氧化应激、细胞增殖、细胞凋亡等生理病理过程中发挥作用。一系列研究表明，核因子κB的阻断可保护心脏免受急性心肌缺血损伤和炎性反应的影响。

背景：针刺心俞穴能显著改善急性心肌缺血的心功能，保护心肌细胞，但心俞穴位埋线处理对急性心肌缺血心功能的效果及机制尚不明确。核因子κB激活常出现P65亚型的核内易位，核因子κB信号通路的活化标志是P65水平升高。
目的：探究心俞穴位埋线预处理对急性心肌缺血大鼠心功能及白细胞介素10、肿瘤坏死因子α、P65基因和蛋白表达水平的影响。
方法：采用随机数字表法将32只雄性SD大鼠分为空白组、模型组、心俞穴组、非经非穴组，每组8只，后3组构建急性心肌缺血大鼠模型。心俞穴组大鼠心俞穴位埋线14 d，随后背部皮下注射盐酸异丙肾上腺素构建急性心肌缺血大鼠模型；非经非穴组局部埋线14 d，余同上；模型组心俞仅标记，余同上；空白组心俞仅标记，随后背部皮下注射等量生理盐水。造模后24 h检测心电图、心脏超声，腹主动脉取血ELISA法检测血清肌酸激酶和肌酸激酶同工酶水平，随后麻醉处死大鼠取材。苏木精-伊红和TUNEL染色观察心肌组织病理学变化和心肌细胞凋亡情况；RT-qPCR和Western Blot法检测心肌组织肿瘤坏死因子α、白细胞介素10、P65 mRNA及蛋白表达。
结果与结论：①心电图：与空白组比较，模型组、非经非穴组、心俞穴组心电图Ⅱ导联ST段显著抬高；②心脏超声：与模型组比较，心俞穴组左室收缩末期内径显著减小(P < 0.05)，左室射血分数、左室短轴短缩率显著升高(P < 0.05)；③血清肌酸激酶和肌酸激酶同工酶：与模型组比较，心俞穴组显著降低(P < 0.05)；④苏木精-伊红染色：与模型组比较，心俞穴组心肌纤维排列基本整齐，水肿较轻，有少许炎性细胞浸润；⑤TUNEL染色：与模型组比较，心俞穴组心肌细胞凋亡荧光强度显著降低，凋亡率显著降低(P < 0.05)；⑥RT-qPCR、Western Blot：与模型组比较，心俞穴组心肌组织白细胞介素10表达水平显著增高(P < 0.05)、肿瘤坏死因子α、P65表达水平显著降低(P < 0.05)；⑦提示心俞穴位埋线预处理可改善急性心肌缺血大鼠心功能，其作用机制可能与抑制核因子κB信号通路的活化有关。 
https://orcid.org/0009-0006-4570-6682(陈小青)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 心俞, 穴位埋线, 急性心肌缺血, 核因子κB, 异丙肾上腺素, 心功能, P65, 白细胞介素10, 工程化组织构建

Abstract: BACKGROUND: Acupuncture at Xinshu (BL 15) can significantly improve cardiac function and protect myocardial cells in acute myocardial ischemia, but the effect and mechanism of thread embedding treatment at Xinshu (BL 15) on cardiac function in acute myocardial ischemia are yet unclear. Nuclear factor κB activation often appears as an intranuclear translocation of the P65 isoform, and activation of the nuclear factor κB signaling pathway is marked by elevated P65 levels.
OBJECTIVE: To explore the effects of thread embedding pretreatment at Xinshu (BL 15) on cardiac function and the expression levels of interleukin-10, tumor necrosis factor-α, P65 genes and proteins in rats with acute myocardial ischemia.
METHODS: Thirty-two male Sprague-Dawley rats were randomly divided into a blank group, a model group, a Xinshu (BL 15) acupoint group, and a non-meridian/non-acupoint group using a random number table method, with eight rats in each group. Rat models of acute myocardial ischemia were established in the latter three groups. The Xinshu (BL 15) acupoint group had thread embedding at Xinshu (BL 15) for 14 days, followed by subcutaneous injection of isoproterenol hydrochloride into the back to establish an acute myocardial ischemia rat model. The non-meridian/non-acupoint group had local thread embedding for 14 days, and the rest procedures were the same as above. In the model group, Xinshu (BL 15) was only marked, and the rest procedures were the same as above. In the blank group, Xinshu (BL 15) was only marked, and then an equal amount of physiological saline was injected subcutaneously into the back. After 24 hours of modeling, electrocardiogram and cardiac ultrasound were performed. Abdominal aorta blood was extracted for detection of serum creatine kinase and creatine kinase isoenzyme levels using enzyme-linked immunosorbent assay. Subsequently, the rats were euthanized and samples were collected. Hematoxylin-eosin and TUNEL staining were used to observe the pathological changes of myocardial tissue and the apoptosis of myocardial cells. Real-time fluorescence quantitative PCR (RT-qPCR) and western blot were used to detect the mRNA and protein expression of tumor necrosis factor-α, interleukin-10, and P65 in myocardial tissue respectively.
RESULTS AND CONCLUSION: (1) Electrocardiogram: Compared with the blank group, the model group, non-meridian/non-acupoint group, and Xinshu (BL 15) acupoint group had significantly elevated ST segment in lead II of the electrocardiogram. (2) Cardiac ultrasound: Compared with the model group, the Left ventricular end-systolic dimension in the Xinshu (BL 15) acupoint group were significantly reduced (P < 0.05), while left ventricular ejection fraction and left ventricular fractional shortening rate were significantly increased (P < 0.05). (4) Serum creatine kinase and creatine kinase isoenzyme: Compared with the model group, the Xinshu (BL 15) acupoint group showed a significant decrease in serum creatine kinase and creatine kinase isoenzyme levels (P < 0.05). (4) Hematoxylin-eosin staining: Compared with the model group, the arrangement of myocardial fibers in the Xinshu (BL 15) acupoint group was basically neat, with less edema and a small amount of inflammatory cell infiltration. (5) TUNEL staining: Compared with the model group, the fluorescence intensity of myocardial cell apoptosis in the Xinshu (BL 15) acupoint group was significantly reduced, and its apoptosis rate was significantly reduced (P < 0.05). (6) RT-qPCR and western blot: Compared with the model group, the myocardial tissue interleukin-10 level in the Xinshu (BL 15) acupoint group was significantly increased (P < 0.05), while tumor necrosis factor-α and P65 levels were significantly decreased (P < 0.05). These findings indicate that thread embedding pretreatment at Xinshu (BL 15) can improve cardiac function in rats with acute myocardial ischemia, and its mechanism of action may be related to the inhibition of the activation of the nuclear factor-κB signaling pathway.

Key words: Xinshu (BL 15), acupoint embedding, acute myocardial ischemia, nuclear factor-κB, isoproterenol, cardiac function, P65, interleukin-10, engineered tissue construction

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